Uridylate-specific_endoribonuclease Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

Uridylate-specific endoribonuclease (NendoU) (nsp 15)– NendoU cleaves 2′-3′-cyclic phosphates 5′, and it is Mn2+-dependent.2 The functionally active form of the endoribonuclease is a hexamer. The SARS-CoV-2 NendoU protein structure is homologous to SARS-CoV and MERS-CoV. SARS-CoV NendoU inhibitors have good chance of inhibiting the SARS-CoV-2 homolog but inhibitors of MERS-CoV NendoU are unlikely to be effective.25 Based on an in silico study, 2-deoxy-D-glucose can inactivate 3CLpro and nsp15 endoribonuclease.11 One study suggests that the inhaled corticosteroid ciclesonide targets NendoU and blocks coronavirus RNA replication.26

Uridylate-specific endoribonuclease 2,489 Sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
6453SSER48.975492170.0582859751.5521.592H0
6454LLEU82.643631981.0156790071.291.689H0
6455EGLU82.442748091.0099666381.3241.355H2
6456NASN98.5536361.4680986251.2871.136H4
6457VVAL28.96745681-0.510665971.0321.042H1
6458AALA82.96504621.0248187971.1341.068H1
6459FPHE52.591402170.1611086151.2931.295H2
6460NASN99.236641221.487520681.1911.023H1
6461VVAL61.550823620.4158802691.2831.08H1
6462VVAL73.161912410.7460551931.6391.488H2
6463NASN12.97709924-0.9653705371.8611.757H2
6464KLYS48.854961830.0548585532.5291.911H1
6465GGLY94.294897551.3469964042.5081.161C0
6466HHIS57.051024510.2879232051.3761.153C0
6467FPHE64.282844520.4935684871.1020.978C0
6468DASP28.68621937-0.5186632871.491.385C0
6469GGLY73.36279630.7517675621.2771.328C0
6470QGLN15.66894335-0.8888247931.7991.741C1
6471QGLN2.531137003-1.2624137211.8092.303C2
6472GGLY98.995580551.4806658371.7861.479C3
6473EGLU89.112093211.1996172861.6451.604C4
6474VVAL3.013258337-1.2487040351.7371.504C2
6475PPRO94.616311771.3561361941.8441.717E1
6476VVAL62.354359180.4387297451.4851.777E0
6477SSER8.276416231-1.0990399691.7231.544E0
6478IILE46.24347127-0.0194022431.5721.446E1
6479IILE24.18642025-0.6466203511.8551.787E2
6480NASN71.715548410.7049261372.4511.905T2
6481NASN12.93692246-0.966513011.6731.536T1
6482TTHR2.531137003-1.2624137211.7851.336E1
6483VVAL77.501004420.8694423621.6361.152E2
6484YTYR13.74045802-0.9436635351.7551.443E2
6485TTHR30.89594215-0.4558272291.7921.459E2
6486KLYS45.56046605-0.0388242971.8742.388E1
6487VVAL12.81639213-0.9699404322.0992.528E1
6488DASP24.70871836-0.6317681913.1933.013T1
6489GGLY25.9140217-0.5974939782.8742.6T0
6490VVAL40.13660104-0.1930582582.3252.735E1
6491DASP78.103656090.8865794692.1112.069E1
6492VVAL27.76215348-0.5449401841.6392.054E1
6493EGLU8.356769787-1.0967550221.5091.859E2
6494LLEU8.356769787-1.0967550221.6181.485E2
6495FPHE99.116110891.4840932581.5340.978E1
6496EGLU25.47207714-0.6100611891.5931.434C1
6497NASN99.236641221.487520681.5181.077C1
6498KLYS60.265166730.3793211082.2071.984C0
6499TTHR98.9152271.4783808891.8871.562C0
6500TTHR54.479710730.2148048831.9661.729C0
6501LLEU84.170349541.059093011.4651.378C0
6502PPRO99.075934111.4829507851.2291.178H0
6503VVAL12.97709924-0.9653705371.1281.45H0
6504NASN77.862595420.8797246261.3311.376H0
6505VVAL76.898352750.8523052550.9651.053H0
6506AALA99.2768181.4886631531.0230.879H0
6507FPHE84.41141021.0659478531.0160.764H0
6508EGLU99.316994781.4898056270.8750.737H0
6509LLEU94.415427881.3504238250.991.097H0
6510WTRP9.160305344-1.0739055461.0130.813H0
6511AALA92.647649661.3001549791.0210.804H0
6512KLYS93.933306551.336714140.9110.779E0
6513RARG99.116110891.4840932581.2631.132E0
6514NASN25.5524307-0.6077762421.3091.395E0
6515IILE34.83326637-0.3438647981.4221.335C0
6516KLYS9.200482121-1.0727630720.9271.569C0
6517PPRO9.6022499-1.0613383351.4291.033C0
6518VVAL9.079951788-1.0761904941.7871.075E0
6519PPRO99.316994781.4898056271.2020.793C0
6520EGLU24.10606669-0.6489052981.0850.709H0
6521VVAL3.173965448-1.244134141.5170.878H0
6522KLYS40.2169546-0.190773311.5380.934H0
6523IILE61.912414620.4261625331.2130.97H0
6524LLEU86.580956211.1276414371.6070.869H0
6525NASN8.316593009-1.0978974961.8260.747H0
6526NASN77.701888310.8751547311.4830.647H0
6527LLEU98.151868221.4566738871.5260.865H0
6528GGLY66.813981520.5655443351.8370.689C0
6529VVAL69.586179190.6443750261.4980.839C0
6530DASP56.850140620.2822108371.5460.762C0
6531IILE26.03455203-0.5940665561.4930.863E0
6532AALA8.798714343-1.084187811.6390.83E0
6533AALA8.597830454-1.0899001791.931.15E0
6534NASN15.14664524-0.9036769521.8031.292C0
6535TTHR7.995178787-1.1070372861.130.978C0
6536VVAL93.129770991.3138646641.6211.229E0
6537IILE58.617918840.3324796832.171.32E0
6538WTRP99.357171551.4909481011.6180.863E0
6539DASP94.736842111.3595636161.0560.734E0
6540YTYR93.812776221.3332867191.1851.608C0
6541KLYS8.276416231-1.0990399691.2811.417C0
6542RARG37.00281237-0.2821712131.351.571C0
6543DASP7.75411812-1.1138921291.2081.021E0
6544AALA9.642426677-1.0601958611.1060.879E0
6545PPRO63.077541180.4592942731.6680.843E0
6546AALA8.557653676-1.0910426532.5351.049C0
6547HHIS8.155885898-1.1024673913.1011.088C0
6548IILE2.531137003-1.2624137212.731.671C0
6549SSER12.4949779-0.9790802221.4591.021C0
6550TTHR99.156287671.4852357321.1320.777E0
6551IILE30.01205303-0.4809616521.2850.961C0
6552GGLY39.45359582-0.2124803121.8420.834C0
6553VVAL97.428686221.4361093591.6761.037C0
6554CCYS94.495781441.3527087731.7750.964T0
6555SSER6.227400562-1.1573061321.8511.454T0
6556MMET8.396946565-1.0956125481.9141.945T0
6557TTHR99.156287671.4852357321.1990.948E0
6558DASP99.437525111.4932330491.1270.837E0
6559IILE46.64523905-0.0079775051.2461.541E0
6560AALA9.803133789-1.0556259661.4141.057C0
6561KLYS8.838891121-1.0830453361.4312.077C0
6562KLYS8.67818401-1.0876152321.4781.922C0
6563PPRO9.200482121-1.0727630721.4031.007C0
6564TTHR8.396946565-1.0956125481.5241.339C0
6565EGLU8.396946565-1.0956125481.6621.394C0
6566TTHR2.410606669-1.2658411422.21.586G0
6567IILE2.450783447-1.2646986682.5281.678G0
6568CCYS8.477300121-1.0933276012.0381.133G0
6569AALA2.490960225-1.2635561941.681.074G0
6570PPRO2.77219767-1.2555588781.2461.102C0
6571LLEU42.34632382-0.13022221.3851.108C0
6572TTHR10.08437123-1.0476286491.6590.799E0
6573VVAL47.488951390.0160144451.390.825E0
6574FPHE2.571313781-1.2612712471.0970.723E0
6575FPHE71.836078750.7083535581.3830.669E0
6576DASP99.437525111.4932330491.4560.891T0
6577GGLY24.42748092-0.6397655081.6580.969T0
6578RARG62.515066290.443299641.6641.75T0
6579VVAL11.0486139-1.0202092781.7751.228T0
6580DASP2.531137003-1.2624137212.1261.642C0
6581GGLY93.692245881.3298592971.9431.165H0
6582QGLN8.396946565-1.0956125481.771.219H0
6583VVAL8.316593009-1.0978974961.7311.374H1
6584DASP8.437123343-1.0944700742.1171.124H1
6585LLEU8.11570912-1.1036098651.8081.264H0
6586FPHE99.558055441.496660471.780.772H0
6587RARG11.16914423-1.0167818572.161.482H0
6588NASN9.200482121-1.0727630722.2421.476C0
6589AALA29.04781037-0.5083810231.7161.057C0
6590RARG14.10204902-0.9333812711.8851.872C0
6591NASN92.768179991.30358241.5530.822E0
6592GGLY26.79791081-0.5723595551.5270.607E0
6593VVAL82.60345521.0145365331.3370.792E0
6594LLEU63.840899960.4810012751.1820.811E0
6595IILE50.220972280.0937026621.1470.872E0
6596TTHR12.81639213-0.9699404321.3870.88E0
6597EGLU8.437123343-1.0944700741.51.422C0
6598GGLY8.557653676-1.0910426531.6441.178C0
6599SSER8.597830454-1.0899001791.061.369C0
6600VVAL55.765367620.2513640441.0571.214C0
6601KLYS97.227802331.430396992.1372.478C0
6602GGLY13.37886701-0.9539457993.4281.503C0
6603LLEU23.58376858-0.6637574572.9471.355C0
6604QGLN2.732020892-1.2567013523.0522.113C0
6605PPRO8.316593009-1.0978974963.8641.673C0
6606SSER10.76737646-1.0282065952.6191.163E0
6607VVAL18.20008035-0.8168489451.9251.3C0
6608GGLY39.97589393-0.1976281531.7960.741C0
6609PPRO43.02932905-0.1108001451.5510.86C0
6610KLYS2.531137003-1.2624137211.581.759C0
6611QGLN8.396946565-1.0956125481.250.953C0
6612AALA39.81518682-0.2021980481.0430.896E0
6613SSER9.843310566-1.0544834921.1410.89E0
6614LLEU17.59742869-0.8339860511.0141.72E0
6615NASN53.194053840.1782457221.3491.61T0
6616GGLY39.97589393-0.1976281531.9311.656T0
6617VVAL23.34270791-0.67061231.3051.631E0
6618TTHR12.8565689-0.9687979580.9411.08E0
6619LLEU12.69586179-0.9733678531.2741.745E0
6620IILE8.276416231-1.0990399691.1871.183C0
6621GGLY29.36922459-0.4992412321.8281.308C0
6622EGLU8.316593009-1.0978974962.4592.859C0
6623AALA2.450783447-1.2646986682.5491.912C0
6624VVAL50.421856170.0994150312.3651.853C0
6625KLYS24.06588992-0.6500477722.4192.029C0
6626TTHR9.441542788-1.065908231.790.908C0
6627QGLN24.34712736-0.6420504551.5441.537C0
6628FPHE9.160305344-1.0739055461.5070.835C0
6629NASN24.78907192-0.6294832441.2840.828E0
6630YTYR20.73121736-0.7448730961.1190.603E0
6631YTYR2.490960225-1.2635561941.2870.771E0
6632KLYS8.67818401-1.0876152321.460.923E0
6633KLYS8.316593009-1.0978974961.7171.054E0
6634VVAL47.047006830.0034472331.9841.193E0
6635DASP15.18682202-0.9025344782.2691.268T0
6636GGLY78.063479310.8854369952.2611.024T0
6637VVAL3.736440337-1.2281395072.4621.299E0
6638VVAL2.531137003-1.2624137211.8921.101E0
6639QGLN10.88790679-1.0247791731.9351.397C0
6640QGLN9.562073122-1.0624808081.1241.781C0
6641LLEU25.27119325-0.6157735580.9211.005C0
6642PPRO48.332663720.0400063941.0940.928C0
6643EGLU24.78907192-0.6294832441.431.488C0
6644TTHR44.79710727-0.0605312991.2090.758C0
6645YTYR39.57412616-0.2090528911.1610.712C0
6646FPHE48.935315390.0571435011.1610.715C0
6647TTHR98.1920451.4578163610.9980.797C0
6648QGLN98.031337891.4532464660.9240.717C0
6649SSER39.93571716-0.1987706270.7680.771C0
6650RARG99.879469671.505800260.9260.668C0
6651NASN22.90076336-0.6831795121.8221.271T0
6652LLEU17.59742869-0.8339860511.1381.161T0
6653QGLN4.740859783-1.1995776631.7641.458T0
6654EGLU3.374849337-1.2384217712.7711.331T0
6655FPHE99.919646441.5069427341.2870.815C0
6656KLYS14.3029329-0.9276689021.4671.829C0
6657PPRO87.063077541.1413511231.1940.876C0
6658RARG77.420650860.8671574141.3170.896C0
6659SSER82.643631981.0156790071.3011.028H0
6660QGLN10.76737646-1.0282065951.5571.814H1
6661MMET81.076737650.9711225291.240.96H2
6662EGLU99.879469671.505800261.1240.725H2
6663IILE2.973081559-1.2498465091.5671.097H2
6664DASP98.232221781.4589588351.5820.909H2
6665FPHE99.839292891.5046577861.4120.756H1
6666LLEU84.531940541.0693752741.641.179H0
6667EGLU14.82523102-0.9128167421.7291.534H0
6668LLEU26.11490558-0.5917816091.5831.202C1
6669AALA8.597830454-1.0899001791.731.194H2
6670MMET35.47609482-0.3255852171.8241.296H1
6671DASP41.14102049-0.1644964131.8221.367H1
6672EGLU9.03977501-1.0773329681.7251.339H2
6673FPHE99.879469671.505800261.5660.839H2
6674IILE91.000401771.2533135541.7871.164H2
6675EGLU18.80273202-0.7997118381.941.374H2
6676RARG8.959421454-1.0796179151.781.404H2
6677YTYR98.151868221.4566738871.7050.975H2
6678KLYS9.120128566-1.075048021.7511.749C1
6679LLEU83.005222981.0259612711.7441.036T0
6680EGLU75.73322620.8191735151.9371.789T0
6681GGLY9.481719566-1.0647657561.1310.94T0
6682YTYR70.550421860.6717943970.9151.311T0
6683AALA42.3865006-0.1290797261.0890.928H0
6684FPHE77.621534750.8728697830.8061.403H0
6685EGLU81.639212540.9871171621.1181.317H0
6686HHIS99.839292891.5046577861.181.126H0
6687IILE46.00241061-0.0262570850.9181.071H0
6688VVAL77.742065090.8762972050.7830.792H0
6689YTYR94.094013661.3412840350.8890.866C0
6690GGLY99.919646441.5069427340.9550.695C0
6691DASP67.336279630.5803964941.4881.042C0
6692FPHE42.06508638-0.1382195161.6051.813C1
6693SSER66.934511850.5689717571.661.37C1
6694HHIS26.35596625-0.5849267661.8451.795C1
6695SSER2.892728003-1.2521314571.681.577C1
6696QGLN24.94977903-0.6249133491.4831.976C0
6697LLEU73.844917640.7654772481.3141.451C0
6698GGLY99.879469671.505800261.2240.865C0
6699GGLY99.919646441.5069427340.8270.754C0
6700LLEU90.156689431.2293216040.8190.922C0
6701HHIS99.879469671.505800260.8080.892C0
6702LLEU78.786661310.9060015230.731.154C0
6703LLEU76.978706310.8545902030.7570.828H0
6704IILE99.718762561.5012303650.9370.888H0
6705GGLY58.33668140.3244823671.1470.601H0
6706LLEU45.64081961-0.0365393490.9861.146H0
6707AALA10.36560868-1.0396313330.8060.886H0
6708KLYS29.65046203-0.4912439161.3241.178H0
6709RARG24.30695058-0.6431929291.1580.823H0
6710FPHE2.450783447-1.2646986680.8731.277H0
6711KLYS17.71795902-0.830558631.4131.979H0
6712EGLU24.82924869-0.628340771.8771.55C0
6713SSER23.70429892-0.6603300361.7221.237C0
6714PPRO7.794294898-1.1127496551.7851.515E0
6715FPHE2.732020892-1.2567013521.9391.483E0
6716EGLU3.013258337-1.2487040351.8771.712E0
6717LLEU8.437123343-1.0944700741.8181.677E0
6718EGLU56.408196060.2696436252.0911.553E0
6719DASP8.356769787-1.0967550221.5671.09E0
6720FPHE23.50341503-0.6660424051.6041.362C0
6721IILE9.40136601-1.0670507031.2841.661C0
6722PPRO9.441542788-1.065908230.9531.137C0
6723MMET8.11570912-1.1036098650.8351.3C0
6724DASP77.340297310.8648724671.3650.786C0
6725SSER84.451586981.0670903270.9110.812C0
6726TTHR63.157894740.4615792211.0730.812C0
6727VVAL27.28003214-0.5586498690.7180.988C0
6728KLYS41.3017276-0.1599265180.811.591C0
6729NASN25.5524307-0.6077762421.0721.187E0
6730YTYR41.34190438-0.1587840441.3950.906E0
6731FPHE40.49819204-0.1827759941.8971.887E0
6732IILE45.27922861-0.0468216131.7281.1E1
6733TTHR77.340297310.8648724671.8391.512E2
6734DASP24.62836481-0.6340531392.4271.377E1
6735AALA51.024507830.1165521382.5831.832C0
6736QGLN8.236239454-1.1001824432.6842.09C0
6737TTHR26.35596625-0.5849267662.1841.588C0
6738GGLY40.33748493-0.1873458892.1291.539E0
6739SSER72.920851750.7392003511.9771.231E1
6740SSER63.961430290.4844286971.8721.349E2
6741KLYS99.678585781.5000878912.0831.333E1
6742CCYS8.316593009-1.0978974962.0291.332C0
6743VVAL99.558055441.496660471.6711.441C0
6744CCYS99.758939331.5023728391.7781.13E0
6745SSER26.95861792-0.5677896591.4870.805C0
6746VVAL62.31418240.4375872710.650.817C0
6747IILE40.2169546-0.190773310.8361.034C0
6748DASP99.879469671.505800260.9120.581C0
6749LLEU76.536761750.8420229910.9660.988C0
6750LLEU81.558858980.9848322141.1371.324H0
6751LLEU99.758939331.5023728391.0960.789H0
6752DASP99.678585781.5000878911.2291.054H0
6753DASP99.718762561.5012303651.1570.648H0
6754FPHE95.218963441.3732733010.8330.705H0
6755VVAL78.143832860.8877219431.0490.887H0
6756EGLU25.03013258-0.6226284011.4411.033H0
6757IILE68.903173970.6249529721.0920.886H0
6758IILE16.87424669-0.8545505791.2431.25H0
6759KLYS78.425070310.8957192591.5981.645H0
6760SSER70.590598630.6729368711.8181.097H0
6761QGLN25.79349136-0.6009213991.8731.1C0
6762DASP63.921253520.4832862232.5151.442C0
6763LLEU77.54118120.8705848362.2891.294C1
6764SSER24.10606669-0.6489052982.8271.363C2
6765VVAL63.640016070.4752889062.5881.394C2
6766VVAL70.349537970.6660820282.5371.525E2
6767SSER99.718762561.5012303652.281.229E2
6768KLYS99.437525111.4932330492.2121.402E2
6769VVAL99.558055441.496660471.8911.449E1
6770VVAL62.474889510.4421571661.7881.042E0
6771KLYS25.43190036-0.6112036631.3951.441E0
6772VVAL95.982322221.3949803031.0481.221E0
6773TTHR8.597830454-1.0899001791.1050.779E0
6774IILE53.877059060.1976677760.9390.759E0
6775DASP99.799116111.5035153131.0720.557T0
6776YTYR25.27119325-0.6157735581.1090.659T0
6777TTHR19.52591402-0.779147311.3591.009E0
6778EGLU8.437123343-1.0944700740.9931.082E0
6779IILE44.11410205-0.0799533530.7760.963E0
6780SSER8.758537565-1.0853302841.3031.04E0
6781FPHE63.157894740.4615792211.5160.716E0
6782MMET99.558055441.496660471.941.291E0
6783LLEU83.005222981.0259612711.9171.043E1
6784WTRP99.839292891.5046577862.2411.683E2
6785CCYS78.304539980.8922918382.4431.081E2
6786KLYS58.175974290.3199124712.3191.968E2
6787DASP77.661711530.8740122572.3991.607T2
6788GGLY42.58738449-0.1233673572.3811.297T2
6789HHIS12.17356368-0.9882200122.2771.568E2
6790VVAL51.506629170.1302618232.1251.103E2
6791EGLU8.396946565-1.0956125482.1521.308E1
6792TTHR99.718762561.5012303651.9331.276E0
6793FPHE78.62595420.9014316281.580.838E0
6794YTYR99.6384091.4989454181.3950.968E0
6795PPRO99.558055441.496660471.3850.966E0
6796KLYS10.08437123-1.0476286492.3852.563C0
6797LLEU97.709923661.4441066764.2393.405C0
6798QGLN99.6384091.4989454186.6876.048C0

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

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