ORF8 Google Drive Data
ORF8– deletion of ORF8 transcription-regulatory sequence (TRS) enhances the downstream transcription of the N gene.30 ORF8 evolves rapidly which helps counter the host immune response.31 ORF8 does not have any homologous proteins in SARS-CoV.32

ORF8 35 Sequences
Amino Acid | AA | AA | Conservation | Conservation Z-score | RMSD_CA | RMSF[A] | Secondary Structure | DCCM >0.9 |
1 | M | MET | 100 | 1.528571127 | 9.15 | 1.885 | C | 0 |
2 | K | LYS | 100 | 1.528571127 | 4.258 | 1.281 | C | 0 |
3 | F | PHE | 22.85714286 | -1.027130957 | 4.256 | 1.41 | C | 0 |
4 | L | LEU | 100 | 1.528571127 | 4.745 | 1.643 | C | 0 |
5 | V | VAL | 20 | -1.121786589 | 4.239 | 1.552 | C | 0 |
6 | F | PHE | 20 | -1.121786589 | 5.005 | 1.913 | C | 0 |
7 | L | LEU | 65.71428571 | 0.392703534 | 5.17 | 2.098 | C | 0 |
8 | G | GLY | 62.85714286 | 0.298047901 | 2.773 | 0.918 | H | 0 |
9 | I | ILE | 20 | -1.121786589 | 2.757 | 1.549 | H | 0 |
10 | I | ILE | 51.42857143 | -0.08057463 | 6.463 | 2.211 | H | 0 |
11 | T | THR | 54.28571429 | 0.014081003 | 7.183 | 1.349 | H | 0 |
12 | T | THR | 20 | -1.121786589 | 5.222 | 1.454 | H | 0 |
13 | V | VAL | 62.85714286 | 0.298047901 | 5.181 | 2.418 | H | 0 |
14 | A | ALA | 17.14285714 | -1.216442222 | 6.256 | 2.458 | C | 0 |
15 | A | ALA | 20 | -1.121786589 | 6.806 | 2.59 | C | 1 |
16 | F | PHE | 25.71428571 | -0.932475324 | 6.835 | 3.373 | C | 2 |
17 | H | HIS | 62.85714286 | 0.298047901 | 7.142 | 3.156 | C | 3 |
18 | Q | GLN | 20 | -1.121786589 | 6.921 | 4.487 | C | 2 |
19 | E | GLU | 62.85714286 | 0.298047901 | 7.59 | 3.434 | C | 3 |
20 | C | CYS | 62.85714286 | 0.298047901 | 6.564 | 2.494 | C | 2 |
21 | S | SER | 60 | 0.203392269 | 4.117 | 2.241 | H | 1 |
22 | L | LEU | 20 | -1.121786589 | 4.261 | 2.809 | H | 0 |
23 | Q | GLN | 62.85714286 | 0.298047901 | 4.904 | 2.205 | H | 0 |
24 | S | SER | 20 | -1.121786589 | 3.385 | 1.516 | H | 0 |
25 | C | CYS | 100 | 1.528571127 | 2.672 | 1.305 | H | 0 |
26 | T | THR | 14.28571429 | -1.311097855 | 4.033 | 1.676 | H | 0 |
27 | Q | GLN | 20 | -1.121786589 | 3.646 | 2.056 | H | 0 |
28 | H | HIS | 20 | -1.121786589 | 2.712 | 1.915 | C | 0 |
29 | Q | GLN | 62.85714286 | 0.298047901 | 3.3 | 2.092 | C | 0 |
30 | P | PRO | 97.14285714 | 1.433915494 | 2.816 | 1.759 | C | 0 |
31 | Y | TYR | 54.28571429 | 0.014081003 | 4.875 | 2.865 | C | 0 |
32 | V | VAL | 57.14285714 | 0.108736636 | 3.932 | 1.681 | C | 0 |
33 | V | VAL | 20 | -1.121786589 | 5.157 | 1.959 | C | 0 |
34 | D | ASP | 20 | -1.121786589 | 4.162 | 1.645 | C | 0 |
35 | D | ASP | 82.85714286 | 0.96063733 | 7.242 | 1.935 | C | 0 |
36 | P | PRO | 100 | 1.528571127 | 6.931 | 2.214 | C | 1 |
37 | C | CYS | 97.14285714 | 1.433915494 | 9.084 | 2.555 | C | 2 |
38 | P | PRO | 100 | 1.528571127 | 6.32 | 2.498 | C | 2 |
39 | I | ILE | 62.85714286 | 0.298047901 | 6.519 | 2.562 | C | 2 |
40 | H | HIS | 62.85714286 | 0.298047901 | 5.438 | 2.151 | C | 1 |
41 | F | PHE | 20 | -1.121786589 | 3.282 | 1.772 | T | 0 |
42 | Y | TYR | 62.85714286 | 0.298047901 | 3.402 | 2.88 | T | 0 |
43 | S | SER | 62.85714286 | 0.298047901 | 5.9 | 2.492 | T | 0 |
44 | K | LYS | 17.14285714 | -1.216442222 | 5.048 | 3.244 | T | 1 |
45 | W | TRP | 100 | 1.528571127 | 6.647 | 2.601 | C | 2 |
46 | Y | TYR | 22.85714286 | -1.027130957 | 6.695 | 2.403 | C | 1 |
47 | I | ILE | 80 | 0.865981698 | 5.862 | 2.091 | C | 0 |
48 | R | ARG | 57.14285714 | 0.108736636 | 5.758 | 1.501 | C | 0 |
49 | V | VAL | 20 | -1.121786589 | 3.791 | 1.659 | C | 0 |
50 | G | GLY | 62.85714286 | 0.298047901 | 3.115 | 1.626 | T | 0 |
51 | A | ALA | 20 | -1.121786589 | 2.545 | 1.514 | T | 0 |
52 | R | ARG | 97.14285714 | 1.433915494 | 4.484 | 2.024 | T | 0 |
53 | K | LYS | 62.85714286 | 0.298047901 | 5.282 | 2.975 | T | 0 |
54 | S | SER | 62.85714286 | 0.298047901 | 5.243 | 2.448 | C | 0 |
55 | A | ALA | 62.85714286 | 0.298047901 | 5.173 | 2.159 | C | 0 |
56 | P | PRO | 20 | -1.121786589 | 5.079 | 2.127 | C | 0 |
57 | L | LEU | 62.85714286 | 0.298047901 | 5.247 | 2.297 | C | 0 |
58 | I | ILE | 20 | -1.121786589 | 4.455 | 2.018 | C | 0 |
59 | E | GLU | 20 | -1.121786589 | 4.409 | 2.042 | T | 0 |
60 | L | LEU | 62.85714286 | 0.298047901 | 4.644 | 2.102 | T | 0 |
61 | C | CYS | 62.85714286 | 0.298047901 | 2.458 | 1.508 | T | 0 |
62 | V | VAL | 14.28571429 | -1.311097855 | 1.534 | 2.046 | T | 0 |
63 | D | ASP | 20 | -1.121786589 | 2.3 | 2.37 | C | 0 |
64 | E | GLU | 42.85714286 | -0.364541528 | 2.423 | 2.503 | C | 0 |
65 | A | ALA | 14.28571429 | -1.311097855 | 2.373 | 1.901 | T | 0 |
66 | G | GLY | 62.85714286 | 0.298047901 | 6.473 | 1.765 | T | 0 |
67 | S | SER | 20 | -1.121786589 | 5.115 | 1.718 | T | 0 |
68 | K | LYS | 20 | -1.121786589 | 6.514 | 3.063 | T | 0 |
69 | S | SER | 20 | -1.121786589 | 8.29 | 3.079 | C | 1 |
70 | P | PRO | 62.85714286 | 0.298047901 | 7.705 | 3.563 | C | 1 |
71 | I | ILE | 60 | 0.203392269 | 6.959 | 2.744 | C | 2 |
72 | Q | GLN | 22.85714286 | -1.027130957 | 7.341 | 4.306 | C | 3 |
73 | Y | TYR | 62.85714286 | 0.298047901 | 8.183 | 3.743 | C | 3 |
74 | I | ILE | 20 | -1.121786589 | 9.614 | 4.939 | C | 2 |
75 | D | ASP | 20 | -1.121786589 | 9.796 | 4.258 | C | 0 |
76 | I | ILE | 20 | -1.121786589 | 10.1 | 4.641 | T | 0 |
77 | G | GLY | 62.85714286 | 0.298047901 | 8.801 | 3.165 | T | 0 |
78 | N | ASN | 62.85714286 | 0.298047901 | 8.438 | 3.872 | T | 0 |
79 | Y | TYR | 62.85714286 | 0.298047901 | 6.223 | 2.249 | T | 0 |
80 | T | THR | 60 | 0.203392269 | 6.104 | 2.936 | C | 0 |
81 | V | VAL | 20 | -1.121786589 | 4.881 | 2.261 | C | 0 |
82 | S | SER | 57.14285714 | 0.108736636 | 1.321 | 1.358 | C | 0 |
83 | C | CYS | 100 | 1.528571127 | 1.217 | 1.099 | C | 0 |
84 | L | LEU | 2.857142857 | -1.689720386 | 1.36 | 1.783 | C | 0 |
85 | P | PRO | 100 | 1.528571127 | 1.907 | 1.662 | C | 0 |
86 | F | PHE | 25.71428571 | -0.932475324 | 2.247 | 2.412 | C | 0 |
87 | T | THR | 57.14285714 | 0.108736636 | 2.999 | 1.598 | C | 0 |
88 | I | ILE | 100 | 1.528571127 | 2.85 | 2.179 | C | 0 |
89 | N | ASN | 97.14285714 | 1.433915494 | 3.954 | 2.079 | C | 0 |
90 | C | CYS | 100 | 1.528571127 | 4.51 | 2.245 | C | 0 |
91 | Q | GLN | 100 | 1.528571127 | 4.851 | 2.89 | C | 0 |
92 | E | GLU | 20 | -1.121786589 | 4.694 | 2.187 | T | 0 |
93 | P | PRO | 100 | 1.528571127 | 5.262 | 2.96 | T | 0 |
94 | K | LYS | 20 | -1.121786589 | 4.932 | 2.491 | T | 0 |
95 | L | LEU | 20 | -1.121786589 | 2.812 | 1.807 | T | 0 |
96 | G | GLY | 100 | 1.528571127 | 4.053 | 0.994 | C | 0 |
97 | S | SER | 60 | 0.203392269 | 2.778 | 1.058 | C | 0 |
98 | L | LEU | 100 | 1.528571127 | 1.715 | 1.095 | C | 0 |
99 | V | VAL | 34.28571429 | -0.648508426 | 1.523 | 1.349 | C | 0 |
100 | V | VAL | 62.85714286 | 0.298047901 | 1.527 | 1.347 | C | 0 |
101 | R | ARG | 100 | 1.528571127 | 3.38 | 1.182 | C | 0 |
102 | C | CYS | 97.14285714 | 1.433915494 | 3.366 | 1.518 | C | 0 |
103 | S | SER | 60 | 0.203392269 | 2.545 | 1.604 | C | 0 |
104 | F | PHE | 17.14285714 | -1.216442222 | 1.813 | 1.521 | C | 1 |
105 | Y | TYR | 20 | -1.121786589 | 3.451 | 2.316 | C | 2 |
106 | E | GLU | 20 | -1.121786589 | 3.055 | 2.563 | C | 2 |
107 | D | ASP | 60 | 0.203392269 | 4.039 | 2.548 | C | 2 |
108 | F | PHE | 60 | 0.203392269 | 5.06 | 2.184 | C | 2 |
109 | L | LEU | 20 | -1.121786589 | 4.842 | 2.171 | C | 1 |
110 | E | GLU | 60 | 0.203392269 | 5.062 | 1.27 | C | 0 |
111 | Y | TYR | 42.85714286 | -0.364541528 | 4.957 | 1.587 | C | 0 |
112 | H | HIS | 62.85714286 | 0.298047901 | 4.88 | 1.364 | C | 0 |
113 | D | ASP | 100 | 1.528571127 | 3.594 | 1.072 | C | 0 |
114 | V | VAL | 77.14285714 | 0.771326065 | 4.205 | 1.59 | C | 0 |
115 | R | ARG | 62.85714286 | 0.298047901 | 2.934 | 1.017 | C | 0 |
116 | V | VAL | 100 | 1.528571127 | 1.922 | 1.328 | C | 0 |
117 | V | VAL | 85.71428571 | 1.055292963 | 3.005 | 1.102 | C | 0 |
118 | L | LEU | 100 | 1.528571127 | 1.825 | 1.623 | C | 0 |
119 | D | ASP | 62.85714286 | 0.298047901 | 3.182 | 1.181 | C | 0 |
120 | F | PHE | 77.14285714 | 0.771326065 | 3.243 | 2.807 | C | 0 |
121 | I | ILE | 57.14285714 | 0.108736636 | 2.881 | 2.586 | C | 0 |
30. Discovery of a 382-nt deletion during the early evolution of SARS-CoV-2 | bioRxiv [Internet]. [cited 2020 Apr 9];Available from: https://www.biorxiv.org/content/10.1101/2020.03.11.987222v1.abstract
31. Tan Y, Schneider T, Leong M, Aravind L, Zhang D. Novel Immunoglobulin Domain Proteins Provide Insights into Evolution and Pathogenesis Mechanisms of SARS-Related Coronaviruses. bioRxiv 2020;2020.03.04.977736.
32. Xu J, Zhao S, Teng T, et al. Systematic Comparison of Two Animal-to-Human Transmitted Human Coronaviruses: SARS-CoV-2 and SARS-CoV. Viruses 2020;12(2):244.