ORF8 Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

ORF8– deletion of ORF8 transcription-regulatory sequence (TRS) enhances the downstream transcription of the N gene.30 ORF8 evolves rapidly which helps counter the host immune response.31 ORF8 does not have any homologous proteins in SARS-CoV.32   

ORF8 35 Sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
1MMET1001.5285711279.151.885C0
2KLYS1001.5285711274.2581.281C0
3FPHE22.85714286-1.0271309574.2561.41C0
4LLEU1001.5285711274.7451.643C0
5VVAL20-1.1217865894.2391.552C0
6FPHE20-1.1217865895.0051.913C0
7LLEU65.714285710.3927035345.172.098C0
8GGLY62.857142860.2980479012.7730.918H0
9IILE20-1.1217865892.7571.549H0
10IILE51.42857143-0.080574636.4632.211H0
11TTHR54.285714290.0140810037.1831.349H0
12TTHR20-1.1217865895.2221.454H0
13VVAL62.857142860.2980479015.1812.418H0
14AALA17.14285714-1.2164422226.2562.458C0
15AALA20-1.1217865896.8062.59C1
16FPHE25.71428571-0.9324753246.8353.373C2
17HHIS62.857142860.2980479017.1423.156C3
18QGLN20-1.1217865896.9214.487C2
19EGLU62.857142860.2980479017.593.434C3
20CCYS62.857142860.2980479016.5642.494C2
21SSER600.2033922694.1172.241H1
22LLEU20-1.1217865894.2612.809H0
23QGLN62.857142860.2980479014.9042.205H0
24SSER20-1.1217865893.3851.516H0
25CCYS1001.5285711272.6721.305H0
26TTHR14.28571429-1.3110978554.0331.676H0
27QGLN20-1.1217865893.6462.056H0
28HHIS20-1.1217865892.7121.915C0
29QGLN62.857142860.2980479013.32.092C0
30PPRO97.142857141.4339154942.8161.759C0
31YTYR54.285714290.0140810034.8752.865C0
32VVAL57.142857140.1087366363.9321.681C0
33VVAL20-1.1217865895.1571.959C0
34DASP20-1.1217865894.1621.645C0
35DASP82.857142860.960637337.2421.935C0
36PPRO1001.5285711276.9312.214C1
37CCYS97.142857141.4339154949.0842.555C2
38PPRO1001.5285711276.322.498C2
39IILE62.857142860.2980479016.5192.562C2
40HHIS62.857142860.2980479015.4382.151C1
41FPHE20-1.1217865893.2821.772T0
42YTYR62.857142860.2980479013.4022.88T0
43SSER62.857142860.2980479015.92.492T0
44KLYS17.14285714-1.2164422225.0483.244T1
45WTRP1001.5285711276.6472.601C2
46YTYR22.85714286-1.0271309576.6952.403C1
47IILE800.8659816985.8622.091C0
48RARG57.142857140.1087366365.7581.501C0
49VVAL20-1.1217865893.7911.659C0
50GGLY62.857142860.2980479013.1151.626T0
51AALA20-1.1217865892.5451.514T0
52RARG97.142857141.4339154944.4842.024T0
53KLYS62.857142860.2980479015.2822.975T0
54SSER62.857142860.2980479015.2432.448C0
55AALA62.857142860.2980479015.1732.159C0
56PPRO20-1.1217865895.0792.127C0
57LLEU62.857142860.2980479015.2472.297C0
58IILE20-1.1217865894.4552.018C0
59EGLU20-1.1217865894.4092.042T0
60LLEU62.857142860.2980479014.6442.102T0
61CCYS62.857142860.2980479012.4581.508T0
62VVAL14.28571429-1.3110978551.5342.046T0
63DASP20-1.1217865892.32.37C0
64EGLU42.85714286-0.3645415282.4232.503C0
65AALA14.28571429-1.3110978552.3731.901T0
66GGLY62.857142860.2980479016.4731.765T0
67SSER20-1.1217865895.1151.718T0
68KLYS20-1.1217865896.5143.063T0
69SSER20-1.1217865898.293.079C1
70PPRO62.857142860.2980479017.7053.563C1
71IILE600.2033922696.9592.744C2
72QGLN22.85714286-1.0271309577.3414.306C3
73YTYR62.857142860.2980479018.1833.743C3
74IILE20-1.1217865899.6144.939C2
75DASP20-1.1217865899.7964.258C0
76IILE20-1.12178658910.14.641T0
77GGLY62.857142860.2980479018.8013.165T0
78NASN62.857142860.2980479018.4383.872T0
79YTYR62.857142860.2980479016.2232.249T0
80TTHR600.2033922696.1042.936C0
81VVAL20-1.1217865894.8812.261C0
82SSER57.142857140.1087366361.3211.358C0
83CCYS1001.5285711271.2171.099C0
84LLEU2.857142857-1.6897203861.361.783C0
85PPRO1001.5285711271.9071.662C0
86FPHE25.71428571-0.9324753242.2472.412C0
87TTHR57.142857140.1087366362.9991.598C0
88IILE1001.5285711272.852.179C0
89NASN97.142857141.4339154943.9542.079C0
90CCYS1001.5285711274.512.245C0
91QGLN1001.5285711274.8512.89C0
92EGLU20-1.1217865894.6942.187T0
93PPRO1001.5285711275.2622.96T0
94KLYS20-1.1217865894.9322.491T0
95LLEU20-1.1217865892.8121.807T0
96GGLY1001.5285711274.0530.994C0
97SSER600.2033922692.7781.058C0
98LLEU1001.5285711271.7151.095C0
99VVAL34.28571429-0.6485084261.5231.349C0
100VVAL62.857142860.2980479011.5271.347C0
101RARG1001.5285711273.381.182C0
102CCYS97.142857141.4339154943.3661.518C0
103SSER600.2033922692.5451.604C0
104FPHE17.14285714-1.2164422221.8131.521C1
105YTYR20-1.1217865893.4512.316C2
106EGLU20-1.1217865893.0552.563C2
107DASP600.2033922694.0392.548C2
108FPHE600.2033922695.062.184C2
109LLEU20-1.1217865894.8422.171C1
110EGLU600.2033922695.0621.27C0
111YTYR42.85714286-0.3645415284.9571.587C0
112HHIS62.857142860.2980479014.881.364C0
113DASP1001.5285711273.5941.072C0
114VVAL77.142857140.7713260654.2051.59C0
115RARG62.857142860.2980479012.9341.017C0
116VVAL1001.5285711271.9221.328C0
117VVAL85.714285711.0552929633.0051.102C0
118LLEU1001.5285711271.8251.623C0
119DASP62.857142860.2980479013.1821.181C0
120FPHE77.142857140.7713260653.2432.807C0
121IILE57.142857140.1087366362.8812.586C0

30.         Discovery of a 382-nt deletion during the early evolution of SARS-CoV-2 | bioRxiv [Internet]. [cited 2020 Apr 9];Available from: https://www.biorxiv.org/content/10.1101/2020.03.11.987222v1.abstract

31.         Tan Y, Schneider T, Leong M, Aravind L, Zhang D. Novel Immunoglobulin Domain Proteins Provide Insights into Evolution and Pathogenesis Mechanisms of SARS-Related Coronaviruses. bioRxiv 2020;2020.03.04.977736.

32.         Xu J, Zhao S, Teng T, et al. Systematic Comparison of Two Animal-to-Human Transmitted Human Coronaviruses: SARS-CoV-2 and SARS-CoV. Viruses 2020;12(2):244.