ORF8 Google Drive Data

ORF8– deletion of ORF8 transcription-regulatory sequence (TRS) enhances the downstream transcription of the N gene.³⁰ ORF8 evolves rapidly which helps counter the host immune response.³¹ ORF8 does not have any homologous proteins in SARS-CoV.³²   

ORF8 35 Sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
1	M	MET	100	1.528571127	9.15	1.885	C	0
2	K	LYS	100	1.528571127	4.258	1.281	C	0
3	F	PHE	22.85714286	-1.027130957	4.256	1.41	C	0
4	L	LEU	100	1.528571127	4.745	1.643	C	0
5	V	VAL	20	-1.121786589	4.239	1.552	C	0
6	F	PHE	20	-1.121786589	5.005	1.913	C	0
7	L	LEU	65.71428571	0.392703534	5.17	2.098	C	0
8	G	GLY	62.85714286	0.298047901	2.773	0.918	H	0
9	I	ILE	20	-1.121786589	2.757	1.549	H	0
10	I	ILE	51.42857143	-0.08057463	6.463	2.211	H	0
11	T	THR	54.28571429	0.014081003	7.183	1.349	H	0
12	T	THR	20	-1.121786589	5.222	1.454	H	0
13	V	VAL	62.85714286	0.298047901	5.181	2.418	H	0
14	A	ALA	17.14285714	-1.216442222	6.256	2.458	C	0
15	A	ALA	20	-1.121786589	6.806	2.59	C	1
16	F	PHE	25.71428571	-0.932475324	6.835	3.373	C	2
17	H	HIS	62.85714286	0.298047901	7.142	3.156	C	3
18	Q	GLN	20	-1.121786589	6.921	4.487	C	2
19	E	GLU	62.85714286	0.298047901	7.59	3.434	C	3
20	C	CYS	62.85714286	0.298047901	6.564	2.494	C	2
21	S	SER	60	0.203392269	4.117	2.241	H	1
22	L	LEU	20	-1.121786589	4.261	2.809	H	0
23	Q	GLN	62.85714286	0.298047901	4.904	2.205	H	0
24	S	SER	20	-1.121786589	3.385	1.516	H	0
25	C	CYS	100	1.528571127	2.672	1.305	H	0
26	T	THR	14.28571429	-1.311097855	4.033	1.676	H	0
27	Q	GLN	20	-1.121786589	3.646	2.056	H	0
28	H	HIS	20	-1.121786589	2.712	1.915	C	0
29	Q	GLN	62.85714286	0.298047901	3.3	2.092	C	0
30	P	PRO	97.14285714	1.433915494	2.816	1.759	C	0
31	Y	TYR	54.28571429	0.014081003	4.875	2.865	C	0
32	V	VAL	57.14285714	0.108736636	3.932	1.681	C	0
33	V	VAL	20	-1.121786589	5.157	1.959	C	0
34	D	ASP	20	-1.121786589	4.162	1.645	C	0
35	D	ASP	82.85714286	0.96063733	7.242	1.935	C	0
36	P	PRO	100	1.528571127	6.931	2.214	C	1
37	C	CYS	97.14285714	1.433915494	9.084	2.555	C	2
38	P	PRO	100	1.528571127	6.32	2.498	C	2
39	I	ILE	62.85714286	0.298047901	6.519	2.562	C	2
40	H	HIS	62.85714286	0.298047901	5.438	2.151	C	1
41	F	PHE	20	-1.121786589	3.282	1.772	T	0
42	Y	TYR	62.85714286	0.298047901	3.402	2.88	T	0
43	S	SER	62.85714286	0.298047901	5.9	2.492	T	0
44	K	LYS	17.14285714	-1.216442222	5.048	3.244	T	1
45	W	TRP	100	1.528571127	6.647	2.601	C	2
46	Y	TYR	22.85714286	-1.027130957	6.695	2.403	C	1
47	I	ILE	80	0.865981698	5.862	2.091	C	0
48	R	ARG	57.14285714	0.108736636	5.758	1.501	C	0
49	V	VAL	20	-1.121786589	3.791	1.659	C	0
50	G	GLY	62.85714286	0.298047901	3.115	1.626	T	0
51	A	ALA	20	-1.121786589	2.545	1.514	T	0
52	R	ARG	97.14285714	1.433915494	4.484	2.024	T	0
53	K	LYS	62.85714286	0.298047901	5.282	2.975	T	0
54	S	SER	62.85714286	0.298047901	5.243	2.448	C	0
55	A	ALA	62.85714286	0.298047901	5.173	2.159	C	0
56	P	PRO	20	-1.121786589	5.079	2.127	C	0
57	L	LEU	62.85714286	0.298047901	5.247	2.297	C	0
58	I	ILE	20	-1.121786589	4.455	2.018	C	0
59	E	GLU	20	-1.121786589	4.409	2.042	T	0
60	L	LEU	62.85714286	0.298047901	4.644	2.102	T	0
61	C	CYS	62.85714286	0.298047901	2.458	1.508	T	0
62	V	VAL	14.28571429	-1.311097855	1.534	2.046	T	0
63	D	ASP	20	-1.121786589	2.3	2.37	C	0
64	E	GLU	42.85714286	-0.364541528	2.423	2.503	C	0
65	A	ALA	14.28571429	-1.311097855	2.373	1.901	T	0
66	G	GLY	62.85714286	0.298047901	6.473	1.765	T	0
67	S	SER	20	-1.121786589	5.115	1.718	T	0
68	K	LYS	20	-1.121786589	6.514	3.063	T	0
69	S	SER	20	-1.121786589	8.29	3.079	C	1
70	P	PRO	62.85714286	0.298047901	7.705	3.563	C	1
71	I	ILE	60	0.203392269	6.959	2.744	C	2
72	Q	GLN	22.85714286	-1.027130957	7.341	4.306	C	3
73	Y	TYR	62.85714286	0.298047901	8.183	3.743	C	3
74	I	ILE	20	-1.121786589	9.614	4.939	C	2
75	D	ASP	20	-1.121786589	9.796	4.258	C	0
76	I	ILE	20	-1.121786589	10.1	4.641	T	0
77	G	GLY	62.85714286	0.298047901	8.801	3.165	T	0
78	N	ASN	62.85714286	0.298047901	8.438	3.872	T	0
79	Y	TYR	62.85714286	0.298047901	6.223	2.249	T	0
80	T	THR	60	0.203392269	6.104	2.936	C	0
81	V	VAL	20	-1.121786589	4.881	2.261	C	0
82	S	SER	57.14285714	0.108736636	1.321	1.358	C	0
83	C	CYS	100	1.528571127	1.217	1.099	C	0
84	L	LEU	2.857142857	-1.689720386	1.36	1.783	C	0
85	P	PRO	100	1.528571127	1.907	1.662	C	0
86	F	PHE	25.71428571	-0.932475324	2.247	2.412	C	0
87	T	THR	57.14285714	0.108736636	2.999	1.598	C	0
88	I	ILE	100	1.528571127	2.85	2.179	C	0
89	N	ASN	97.14285714	1.433915494	3.954	2.079	C	0
90	C	CYS	100	1.528571127	4.51	2.245	C	0
91	Q	GLN	100	1.528571127	4.851	2.89	C	0
92	E	GLU	20	-1.121786589	4.694	2.187	T	0
93	P	PRO	100	1.528571127	5.262	2.96	T	0
94	K	LYS	20	-1.121786589	4.932	2.491	T	0
95	L	LEU	20	-1.121786589	2.812	1.807	T	0
96	G	GLY	100	1.528571127	4.053	0.994	C	0
97	S	SER	60	0.203392269	2.778	1.058	C	0
98	L	LEU	100	1.528571127	1.715	1.095	C	0
99	V	VAL	34.28571429	-0.648508426	1.523	1.349	C	0
100	V	VAL	62.85714286	0.298047901	1.527	1.347	C	0
101	R	ARG	100	1.528571127	3.38	1.182	C	0
102	C	CYS	97.14285714	1.433915494	3.366	1.518	C	0
103	S	SER	60	0.203392269	2.545	1.604	C	0
104	F	PHE	17.14285714	-1.216442222	1.813	1.521	C	1
105	Y	TYR	20	-1.121786589	3.451	2.316	C	2
106	E	GLU	20	-1.121786589	3.055	2.563	C	2
107	D	ASP	60	0.203392269	4.039	2.548	C	2
108	F	PHE	60	0.203392269	5.06	2.184	C	2
109	L	LEU	20	-1.121786589	4.842	2.171	C	1
110	E	GLU	60	0.203392269	5.062	1.27	C	0
111	Y	TYR	42.85714286	-0.364541528	4.957	1.587	C	0
112	H	HIS	62.85714286	0.298047901	4.88	1.364	C	0
113	D	ASP	100	1.528571127	3.594	1.072	C	0
114	V	VAL	77.14285714	0.771326065	4.205	1.59	C	0
115	R	ARG	62.85714286	0.298047901	2.934	1.017	C	0
116	V	VAL	100	1.528571127	1.922	1.328	C	0
117	V	VAL	85.71428571	1.055292963	3.005	1.102	C	0
118	L	LEU	100	1.528571127	1.825	1.623	C	0
119	D	ASP	62.85714286	0.298047901	3.182	1.181	C	0
120	F	PHE	77.14285714	0.771326065	3.243	2.807	C	0
121	I	ILE	57.14285714	0.108736636	2.881	2.586	C	0

30.         Discovery of a 382-nt deletion during the early evolution of SARS-CoV-2 | bioRxiv [Internet]. [cited 2020 Apr 9];Available from: https://www.biorxiv.org/content/10.1101/2020.03.11.987222v1.abstract

31.         Tan Y, Schneider T, Leong M, Aravind L, Zhang D. Novel Immunoglobulin Domain Proteins Provide Insights into Evolution and Pathogenesis Mechanisms of SARS-Related Coronaviruses. bioRxiv 2020;2020.03.04.977736.

32.         Xu J, Zhao S, Teng T, et al. Systematic Comparison of Two Animal-to-Human Transmitted Human Coronaviruses: SARS-CoV-2 and SARS-CoV. Viruses 2020;12(2):244.