ORF6 Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

ORF6– an interaction was discovered between SARS-CoV-2 Orf6 and NUP98-RAE1, an interferon-inducible mRNA nuclear export complex that is commonly hijacked or degraded by viruses.8 The loss of ORF3b and truncation/changes in ORF6 in SARS-CoV-2 could signal a reduced capacity of SARS-CoV-2 to interfere with type I IFN responses compared to SARS-CoV. In SARS-CoV ORF6, the N-terminal domain has been shown to have a clear role in its ability to disrupt karyopherin transport. SARS-CoV-2 ORF6 does not effectively block nuclear transport as well as SARS-CoV ORF6.29 This protein could be a determinant of virus virulence and it seems to stimulate cellular DNA synthesis in vitro.2

ORF6 31 Sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
1MMET1000.7861072733.6944.638C0
2FPHE1000.7861072737.4256.128C0
3HHIS93.54838710.6169624988.5934.36C0
4LLEU1000.7861072735.7892.349C0
5VVAL1000.7861072734.5132.439C0
6DASP90.322580650.5323901112.8931.743C0
7FPHE1000.7861072733.3831.935C0
8QGLN1000.7861072732.9721.455C0
9VVAL1000.7861072732.7511.656C0
10TTHR1000.7861072733.8182.023G0
11IILE93.54838710.6169624983.7481.79G0
12AALA1000.7861072733.9681.972G1
13EGLU1000.7861072733.7572.297G2
14IILE83.870967740.3632453364.0212.759T1
15LLEU96.774193550.7015348864.6452.226T0
16LLEU9.677419355-1.5819195755.9354.349T0
17IILE96.774193550.7015348864.0862.801T0
18IILE1000.7861072732.9692.745C0
19MMET1000.7861072732.2942.156C0
20RARG90.322580650.5323901114.0023.37C0
21TTHR93.54838710.6169624985.4513.158C0
22FPHE96.774193550.7015348865.1322.689C0
23KLYS12.90322581-1.4973471876.0142.949C0
24VVAL16.12903226-1.41277487.2861.901C0
25SSER9.677419355-1.5819195758.4652.513T0
26IILE93.54838710.6169624986.4092.344T0
27WTRP87.096774190.4478177244.5222.624T0
28NASN90.322580650.5323901115.4132.514T0
29LLEU93.54838710.6169624984.4221.573H0
30DASP93.54838710.6169624984.871.954H0
31YTYR9.677419355-1.5819195755.3163.114H0
32IILE67.74193548-0.0596166016.1681.855H0
33IILE90.322580650.5323901115.7432.061H0
34NASN9.677419355-1.5819195754.4981.795H0
35LLEU9.677419355-1.5819195753.6841.953H0
36IILE93.54838710.6169624984.251.959H0
37IILE9.677419355-1.5819195754.3222.33H0
38KLYS16.12903226-1.41277483.8712.041H0
39NASN9.677419355-1.5819195753.7791.82H0
40LLEU93.54838710.6169624983.7212.168H0
41SSER12.90322581-1.4973471874.1361.946H0
42KLYS96.774193550.7015348864.342.982H0
43SSER6.451612903-1.6664919621.8521.525H0
44LLEU96.774193550.7015348862.6332.207H0
45TTHR93.54838710.6169624981.2571.293C0
46EGLU6.451612903-1.6664919623.6491.882C0
47NASN19.35483871-1.3282024123.9362.434T1
48KLYS41.93548387-0.73619574.8252.993T1
49YTYR93.54838710.6169624983.5132.269T0
50SSER83.870967740.3632453366.0622.13T0
51QGLN22.58064516-1.2436300254.8912.645C0
52LLEU1000.7861072734.3642.141C0
53DASP1000.7861072735.6542.51C0
54EGLU16.12903226-1.41277485.2032.783C0
55EGLU1000.7861072737.2372.972C0
56QGLN9.677419355-1.5819195759.233.915C0
57PPRO96.774193550.70153488610.3074.325C1
58MMET1000.78610727310.9094.739C2
59EGLU96.774193550.70153488611.8335.859C2
60IILE32.25806452-0.98991286312.0676.703C1
61DASP93.54838710.61696249810.2385.193C0

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

8.            Gordon DE, Jang GM, Bouhaddou M, et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. bioRxiv 2020;2020.03.22.002386.

29.         SARS-CoV-2 is sensitive to type I interferon pretreatment | bioRxiv [Internet]. [cited 2020 Apr 8];Available from: https://www.biorxiv.org/content/10.1101/2020.03.07.982264v2.full