ORF6 Google Drive Data

ORF6– an interaction was discovered between SARS-CoV-2 Orf6 and NUP98-RAE1, an interferon-inducible mRNA nuclear export complex that is commonly hijacked or degraded by viruses.⁸ The loss of ORF3b and truncation/changes in ORF6 in SARS-CoV-2 could signal a reduced capacity of SARS-CoV-2 to interfere with type I IFN responses compared to SARS-CoV. In SARS-CoV ORF6, the N-terminal domain has been shown to have a clear role in its ability to disrupt karyopherin transport. SARS-CoV-2 ORF6 does not effectively block nuclear transport as well as SARS-CoV ORF6.²⁹ This protein could be a determinant of virus virulence and it seems to stimulate cellular DNA synthesis in vitro.²

ORF6 31 Sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
1	M	MET	100	0.786107273	3.694	4.638	C	0
2	F	PHE	100	0.786107273	7.425	6.128	C	0
3	H	HIS	93.5483871	0.616962498	8.593	4.36	C	0
4	L	LEU	100	0.786107273	5.789	2.349	C	0
5	V	VAL	100	0.786107273	4.513	2.439	C	0
6	D	ASP	90.32258065	0.532390111	2.893	1.743	C	0
7	F	PHE	100	0.786107273	3.383	1.935	C	0
8	Q	GLN	100	0.786107273	2.972	1.455	C	0
9	V	VAL	100	0.786107273	2.751	1.656	C	0
10	T	THR	100	0.786107273	3.818	2.023	G	0
11	I	ILE	93.5483871	0.616962498	3.748	1.79	G	0
12	A	ALA	100	0.786107273	3.968	1.972	G	1
13	E	GLU	100	0.786107273	3.757	2.297	G	2
14	I	ILE	83.87096774	0.363245336	4.021	2.759	T	1
15	L	LEU	96.77419355	0.701534886	4.645	2.226	T	0
16	L	LEU	9.677419355	-1.581919575	5.935	4.349	T	0
17	I	ILE	96.77419355	0.701534886	4.086	2.801	T	0
18	I	ILE	100	0.786107273	2.969	2.745	C	0
19	M	MET	100	0.786107273	2.294	2.156	C	0
20	R	ARG	90.32258065	0.532390111	4.002	3.37	C	0
21	T	THR	93.5483871	0.616962498	5.451	3.158	C	0
22	F	PHE	96.77419355	0.701534886	5.132	2.689	C	0
23	K	LYS	12.90322581	-1.497347187	6.014	2.949	C	0
24	V	VAL	16.12903226	-1.4127748	7.286	1.901	C	0
25	S	SER	9.677419355	-1.581919575	8.465	2.513	T	0
26	I	ILE	93.5483871	0.616962498	6.409	2.344	T	0
27	W	TRP	87.09677419	0.447817724	4.522	2.624	T	0
28	N	ASN	90.32258065	0.532390111	5.413	2.514	T	0
29	L	LEU	93.5483871	0.616962498	4.422	1.573	H	0
30	D	ASP	93.5483871	0.616962498	4.87	1.954	H	0
31	Y	TYR	9.677419355	-1.581919575	5.316	3.114	H	0
32	I	ILE	67.74193548	-0.059616601	6.168	1.855	H	0
33	I	ILE	90.32258065	0.532390111	5.743	2.061	H	0
34	N	ASN	9.677419355	-1.581919575	4.498	1.795	H	0
35	L	LEU	9.677419355	-1.581919575	3.684	1.953	H	0
36	I	ILE	93.5483871	0.616962498	4.25	1.959	H	0
37	I	ILE	9.677419355	-1.581919575	4.322	2.33	H	0
38	K	LYS	16.12903226	-1.4127748	3.871	2.041	H	0
39	N	ASN	9.677419355	-1.581919575	3.779	1.82	H	0
40	L	LEU	93.5483871	0.616962498	3.721	2.168	H	0
41	S	SER	12.90322581	-1.497347187	4.136	1.946	H	0
42	K	LYS	96.77419355	0.701534886	4.34	2.982	H	0
43	S	SER	6.451612903	-1.666491962	1.852	1.525	H	0
44	L	LEU	96.77419355	0.701534886	2.633	2.207	H	0
45	T	THR	93.5483871	0.616962498	1.257	1.293	C	0
46	E	GLU	6.451612903	-1.666491962	3.649	1.882	C	0
47	N	ASN	19.35483871	-1.328202412	3.936	2.434	T	1
48	K	LYS	41.93548387	-0.7361957	4.825	2.993	T	1
49	Y	TYR	93.5483871	0.616962498	3.513	2.269	T	0
50	S	SER	83.87096774	0.363245336	6.062	2.13	T	0
51	Q	GLN	22.58064516	-1.243630025	4.891	2.645	C	0
52	L	LEU	100	0.786107273	4.364	2.141	C	0
53	D	ASP	100	0.786107273	5.654	2.51	C	0
54	E	GLU	16.12903226	-1.4127748	5.203	2.783	C	0
55	E	GLU	100	0.786107273	7.237	2.972	C	0
56	Q	GLN	9.677419355	-1.581919575	9.23	3.915	C	0
57	P	PRO	96.77419355	0.701534886	10.307	4.325	C	1
58	M	MET	100	0.786107273	10.909	4.739	C	2
59	E	GLU	96.77419355	0.701534886	11.833	5.859	C	2
60	I	ILE	32.25806452	-0.989912863	12.067	6.703	C	1
61	D	ASP	93.5483871	0.616962498	10.238	5.193	C	0

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

8.            Gordon DE, Jang GM, Bouhaddou M, et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. bioRxiv 2020;2020.03.22.002386.

29.         SARS-CoV-2 is sensitive to type I interferon pretreatment | bioRxiv [Internet]. [cited 2020 Apr 8];Available from: https://www.biorxiv.org/content/10.1101/2020.03.07.982264v2.full