ORF10– the respective region in related beta-coronaviruses, such as SARS-CoV, does not correspond to a translated peptide.2 The novel ORF10 of SARS-CoV-2 interacts with multiple members of a Cullin 2 (CUL2) RING E3 ligase complex, specifically the CUL2ZYG11B complex. ORF10 may bind to the CUL2ZYG11B complex and hijack it for ubiquitination and degradation of restriction factors. The ubiquitin transfer to a substrate requires neddylation of CUL2 via NEDD8-activating enzyme (NAE). NAE can be inhibited by the drug Pevonedistat8
2. I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/
8. Gordon DE, Jang GM, Bouhaddou M, et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. bioRxiv 2020;2020.03.22.002386.