nsp8 Google Drive Data

Non-structural protein 8 (nsp8)– nsp8 interacts with nsp7 to form a hexadecamer that may act as a primase.² Nsp12-nsp7-nsp8 complex of SARS-CoV-2 is essential for viral replication and transcription and is a potential target to fight against the virus. Seven compounds (Saquinavir, Tipranavir, Lonafarnib, Tegobuvir, Olysio, Filibuvir, and Cepharanthine) were found to interact well with nsp12-nsp7-nsp8 indicating potential as drug candidates.¹⁴

Dynamics Data for Interaction

nsp8 3,339 Sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
3943	A	ALA	46.87032046	-0.084730397	6.887	5.449	H	2
3944	I	ILE	8.565438754	-1.316139018	4.496	4.787	H	2
3945	A	ALA	42.64749925	-0.22048381	5.936	4.718	C	1
3946	S	SER	77.44833783	0.898278361	5.008	5.148	C	1
3947	E	GLU	62.41389638	0.414957698	5.624	7.207	C	2
3948	F	PHE	71.75801138	0.71534823	6.304	7.398	C	4
3949	S	SER	48.21802935	-0.04140484	6.46	5.885	C	3
3950	S	SER	12.42887092	-1.191939087	5.401	4.578	H	3
3951	L	LEU	60.88649296	0.3658554	6.258	6.011	H	6
3952	P	PRO	57.74183887	0.264762433	7.077	5.386	H	3
3953	S	SER	77.08894879	0.886724879	5.654	3.628	H	2
3954	Y	TYR	51.84186882	0.07509277	4.928	4.148	H	2
3955	A	ALA	47.46930219	-0.065474594	6.298	4.503	H	4
3956	A	ALA	24.79784367	-0.794306748	6.638	3.998	H	3
3957	F	PHE	2.425876011	-1.513511002	5.458	3.317	H	3
3958	A	ALA	8.625336927	-1.314213437	5.926	4.028	H	3
3959	T	THR	14.07607068	-1.138985627	7.142	4.583	H	4
3960	A	ALA	99.61066187	1.610743083	7.229	4.108	H	4
3961	Q	GLN	32.34501348	-0.551683627	6.922	4.662	H	9
3962	E	GLU	8.924827793	-1.304585536	7.782	5.518	H	8
3963	A	ALA	35.93890386	-0.436148807	8.641	5.269	H	7
3964	Y	TYR	80.65289009	1.001296909	8.651	5.863	H	15
3965	E	GLU	57.38244984	0.253208951	8.932	6.318	H	9
3966	Q	GLN	8.77508236	-1.309399487	9.711	6.745	H	11
3967	A	ALA	79.48487571	0.963748092	10.146	6.669	H	13
3968	V	VAL	34.14195867	-0.493916217	10.29	7.421	H	10
3969	A	ALA	19.76639712	-0.956055496	10.662	7.811	H	10
3970	N	ASN	41.15004492	-0.268623319	11.215	7.847	H	11
3971	G	GLY	82.68942797	1.06676664	11.698	8.39	H	11
3972	D	ASP	32.10542078	-0.559385948	11.529	7.741	C	15
3973	S	SER	48.15813118	-0.04333042	12.38	8.487	H	12
3974	E	GLU	8.6852351	-1.312287857	11.65	8.865	H	11
3975	V	VAL	8.6852351	-1.312287857	11.604	8.403	H	11
3976	V	VAL	33.6328242	-0.51028365	10.665	7.307	H	12
3977	L	LEU	63.37226715	0.445766984	9.616	7.129	H	15
3978	K	LYS	83.61784966	1.096613135	9.351	7.799	H	13
3979	K	LYS	12.51871818	-1.189050716	9.32	6.771	H	11
3980	L	LEU	79.78436658	0.973375994	8.375	5.657	H	14
3981	K	LYS	18.62833184	-0.992641522	7.448	6.187	H	12
3982	K	LYS	77.02905061	0.884799299	7.465	6.423	H	12
3983	S	SER	8.595387841	-1.315176228	7.447	4.828	H	9
3984	L	LEU	8.565438754	-1.316139018	6.179	4.243	H	9
3985	N	ASN	99.64061096	1.611705873	5.634	4.764	H	6
3986	V	VAL	31.68613357	-0.572865011	6.06	4.601	H	5
3987	A	ALA	99.88020365	1.619408194	5.475	3.315	H	2
3988	K	LYS	99.64061096	1.611705873	4.411	4.011	H	2
3989	S	SER	65.79814316	0.523752987	4.657	3.594	H	2
3990	E	GLU	43.78556454	-0.183897784	5	3.624	H	0
3991	F	PHE	56.90326445	0.237804308	3.863	2.952	H	0
3992	D	ASP	53.0098832	0.112641587	3.602	3.105	H	0
3993	R	ARG	69.33213537	0.637362226	4.703	4.446	H	0
3994	D	ASP	62.68343816	0.42362281	5.013	3.612	H	1
3995	A	ALA	37.88559449	-0.373567446	3.979	3.355	H	3
3996	A	ALA	66.63671758	0.550711112	5.102	3.815	H	4
3997	M	MET	8.565438754	-1.316139018	6.29	4.875	H	7
3998	Q	GLN	61.57532195	0.387999574	5.588	4.966	H	10
3999	R	ARG	65.10931417	0.501608813	6.039	5.039	H	12
4000	K	LYS	99.76040731	1.615557034	7.742	5.615	H	12
4001	L	LEU	91.07517221	1.336347886	7.79	5.64	H	14
4002	E	GLU	51.09314166	0.051023016	6.953	5.556	H	16
4003	K	LYS	17.66996107	-1.023450808	8.943	6.164	H	17
4004	M	MET	95.5375861	1.479803621	10.082	6.541	H	18
4005	A	ALA	99.25127284	1.599189601	9.029	6.35	H	20
4006	D	ASP	49.86522911	0.011548619	9.973	6.4	H	21
4007	Q	GLN	63.28241989	0.442878613	12.264	6.815	H	21
4008	A	ALA	99.85025457	1.618445404	12.006	7.196	H	22
4009	M	MET	52.65049416	0.101088105	11.169	7.509	H	22
4010	T	THR	65.28900869	0.507385554	13.483	7.362	H	22
4011	Q	GLN	33.12368973	-0.526651083	14.86	8.077	H	24
4012	M	MET	99.97005091	1.622296565	13.224	8.173	H	24
4013	Y	TYR	99.22132375	1.598226811	13.791	8.67	H	26
4014	K	LYS	95.9868224	1.494245473	15.975	8.867	T	26
4015	Q	GLN	32.82419886	-0.536278984	16.563	9.085	T	25
4016	A	ALA	95.65738245	1.483654781	16.662	8.992	T	23
4017	R	ARG	99.73045822	1.614594244	17.903	9.257	T	23
4018	S	SER	11.62024558	-1.217934421	19.035	9.475	C	23
4019	E	GLU	36.98712189	-0.402451151	20.191	9.906	C	22
4020	D	ASP	73.61485475	0.77504122	21.019	9.96	C	21
4021	K	LYS	47.46930219	-0.065474594	19.578	10.657	C	20
4022	R	ARG	28.75112309	-0.667218447	18.396	10.707	H	18
4023	A	ALA	47.73884397	-0.056809482	15.996	8.513	H	17
4024	K	LYS	99.49086553	1.606891922	13.96	8.253	H	20
4025	V	VAL	51.06319257	0.050060226	13.712	8.445	H	21
4026	T	THR	13.44713986	-1.159204221	13.624	7.523	H	17
4027	S	SER	98.80203654	1.584747749	11.03	6.13	H	18
4028	A	ALA	79.12548667	0.95219461	9.796	6.255	H	18
4029	M	MET	65.7082959	0.520864617	10.962	7.204	H	7
4030	Q	GLN	46.7804732	-0.087618768	9.593	5.603	H	8
4031	T	THR	46.96016771	-0.081842027	7.267	4.608	H	5
4032	M	MET	51.63222522	0.068353239	8.399	6.06	H	5
4033	L	LEU	100	1.623259355	9.638	6.039	H	4
4034	F	PHE	95.44773884	1.47691525	6.977	4.346	H	4
4035	T	THR	8.595387841	-1.315176228	7.21	4.722	H	3
4036	M	MET	99.97005091	1.622296565	10.153	6.366	H	6
4037	L	LEU	61.54537287	0.387036784	10.13	5.435	H	5
4038	R	ARG	54.98652291	0.176185738	9.079	5.233	H	6
4039	K	LYS	69.33213537	0.637362226	11.546	6.966	H	7
4040	L	LEU	84.27672956	1.117794519	14.001	7.439	H	9
4041	D	ASP	99.85025457	1.618445404	13.132	6.84	C	8
4042	N	ASN	46.18149146	-0.106874571	12.487	6.253	C	8
4043	D	ASP	37.25666367	-0.39378604	12.266	6.62	H	8
4044	A	ALA	23.36028751	-0.840520676	11.208	5.554	H	9
4045	L	LEU	62.44384546	0.415920489	10.357	4.729	H	10
4046	N	ASN	68.40371369	0.607515731	10.562	5.409	H	10
4047	N	ASN	10.6918239	-1.247780916	9.839	5.216	H	9
4048	I	ILE	74.03414196	0.788520282	8.482	3.692	H	8
4049	I	ILE	37.52620545	-0.385120928	8.861	4.065	H	5
4050	N	ASN	33.87241689	-0.502581329	9.229	5.211	H	7
4051	N	ASN	46.81042228	-0.086655977	7.57	4.063	H	5
4052	A	ALA	100	1.623259355	6.732	3.397	H	4
4053	R	ARG	45.61245882	-0.125167584	8.392	5.388	H	5
4054	D	ASP	32.70440252	-0.540130145	8.094	5.006	H	5
4055	G	GLY	95.95687332	1.493282683	6.376	4.019	C	3
4056	C	CYS	47.82869122	-0.053921112	5.133	3.394	C	2
4057	V	VAL	66.69661575	0.552636692	5.093	2.964	E	0
4058	P	PRO	99.97005091	1.622296565	5.79	2.642	E	0
4059	L	LEU	94.75890985	1.454771076	6.443	3.399	E	2
4060	N	ASN	21.26385145	-0.907915988	9.126	4.033	E	5
4061	I	ILE	19.70649895	-0.957981076	10.707	3.724	E	7
4062	I	ILE	78.28691225	0.925236486	13.962	4.941	C	8
4063	P	PRO	96.01677149	1.495208263	16.736	5.595	C	7
4064	L	LEU	32.49475891	-0.546869676	19.503	7.03	C	6
4065	T	THR	15.99281222	-1.077367057	19.124	7.366	C	5
4066	T	THR	10.87151842	-1.242004175	16.871	7.657	C	5
4067	A	ALA	54.08805031	0.147302033	13.027	6.473	C	2
4068	A	ALA	10.06289308	-1.26799951	12.858	5.112	E	10
4069	K	LYS	73.88439653	0.783706332	10.817	4.843	E	6
4070	L	LEU	99.64061096	1.611705873	8.032	3.049	E	6
4071	M	MET	8.715184187	-1.311325067	6.245	3.609	E	4
4072	V	VAL	47.73884397	-0.056809482	3.881	2.752	E	0
4073	V	VAL	79.42497754	0.961822512	3.55	3.407	E	4
4074	I	ILE	35.8490566	-0.439037178	5.178	3.781	E	8
4075	P	PRO	76.01078167	0.852064433	7.055	4.657	C	6
4076	D	ASP	93.74064091	1.422036211	10.414	5.967	H	14
4077	Y	TYR	9.104522312	-1.298808795	11.473	6.939	H	13
4078	N	ASN	2.156334232	-1.522176113	12.33	6.86	H	7
4079	T	THR	24.13896376	-0.815488132	9.581	5.084	H	8
4080	Y	TYR	45.79215334	-0.119390843	7.981	5.044	H	12
4081	K	LYS	9.254267745	-1.293994844	10.148	6.813	H	9
4082	N	ASN	8.116202456	-1.33058087	10.394	5.873	H	7
4083	T	THR	9.224318658	-1.294957634	7.426	3.983	E	5
4084	C	CYS	9.314165918	-1.292069264	6.217	4.319	E	7
4085	D	ASP	22.91105121	-0.854962529	7.948	4.752	E	5
4086	G	GLY	28.12219227	-0.68743704	6.817	4.525	T	3
4087	T	THR	2.096436059	-1.524101693	4.094	4.058	T	2
4088	T	THR	12.87810722	-1.177497234	4.029	3.804	E	2
4089	F	PHE	8.924827793	-1.304585536	4.214	2.919	E	2
4090	T	THR	37.8556454	-0.374530237	7.563	4.015	E	2
4091	Y	TYR	96.16651692	1.500022214	8.859	3.248	E	4
4092	A	ALA	79.99401018	0.980115525	12.937	4.548	T	7
4093	S	SER	8.595387841	-1.315176228	14.566	5.392	T	4
4094	A	ALA	38.87391435	-0.341795371	12.118	4.458	E	4
4095	L	LEU	32.10542078	-0.559385948	8.576	4.211	E	2
4096	W	TRP	95.74722971	1.486543152	6.795	3.344	E	3
4097	E	GLU	8.595387841	-1.315176228	5.434	3.673	E	1
4098	I	ILE	89.81731057	1.295910699	4.691	3.495	E	3
4099	Q	GLN	25.48667266	-0.772162575	7.538	4.751	E	4
4100	Q	GLN	9.224318658	-1.294957634	8.145	5.222	E	7
4101	V	VAL	39.95208146	-0.307134925	7.602	4.667	E	13
4102	V	VAL	9.284216831	-1.293032054	8.998	5.441	E	13
4103	D	ASP	72.17729859	0.728827292	10.208	6.245	E	16
4104	A	ALA	35.10032944	-0.463106932	10.097	6.277	T	14
4105	D	ASP	94.33962264	1.441292014	12.96	7.69	T	11
4106	S	SER	8.984725966	-1.302659955	12.158	6.795	E	9
4107	K	LYS	32.70440252	-0.540130145	13.978	7.9	E	11
4108	I	ILE	31.74603175	-0.57093943	12.609	7.064	E	12
4109	V	VAL	64.95956873	0.496794862	11.734	6.326	C	16
4110	Q	GLN	29.43995208	-0.645074273	11.436	6.859	T	9
4111	L	LEU	33.15363881	-0.525688293	9.326	5.291	T	6
4112	S	SER	32.0754717	-0.560348738	12.004	6.948	T	8
4113	E	GLU	51.12309075	0.051985806	13.954	7.564	T	15
4114	I	ILE	31.14705001	-0.590195234	12.069	6.108	C	15
4115	S	SER	14.85474693	-1.113953083	14.345	7.894	T	12
4116	M	MET	8.595387841	-1.315176228	16.311	9.07	T	10
4117	D	ASP	32.46480982	-0.547832466	18.937	9.783	T	12
4118	N	ASN	43.00688829	-0.208930328	17.404	8.814	T	16
4119	S	SER	9.493860437	-1.286292523	15.719	8.315	C	14
4120	P	PRO	8.445642408	-1.319990178	17.058	9.475	C	14
4121	N	ASN	53.63881402	0.13286018	17.396	9.472	C	13
4122	L	LEU	62.53369272	0.418808859	14.333	7.546	C	15
4123	A	ALA	25.63641809	-0.767348624	12.893	7.246	C	14
4124	W	TRP	95.5375861	1.479803621	10.132	6.736	C	15
4125	P	PRO	99.61066187	1.610743083	7.646	5.121	C	13
4126	L	LEU	94.90865529	1.459585027	6.994	5.031	E	17
4127	I	ILE	14.67505241	-1.119729824	5.398	4.038	E	12
4128	V	VAL	30.57801737	-0.608488247	4.666	3.256	E	6
4129	T	THR	19.8262953	-0.954129916	6.323	3.838	E	5
4130	A	ALA	23.51003294	-0.835706726	6.265	3.148	E	6
4131	L	LEU	8.565438754	-1.316139018	8.514	4.478	E	5
4132	R	ARG	99.52081462	1.607854713	9.938	5.262	E	4
4133	A	ALA	10.21263851	-1.263185559	10.591	4.258	C	3
4134	N	ASN	8.595387841	-1.315176228	14.157	6.194	C	1
4135	S	SER	43.24648098	-0.201228007	13.763	6.004	C	1
4136	A	ALA	41.26984127	-0.264772158	16.987	7.27	C	2
4137	V	VAL	60.3474094	0.348525177	14.827	7.047	C	2
4138	K	LYS	65.40880503	0.511236715	13.841	8.623	C	2
4139	L	LEU	76.19047619	0.857841174	13.47	9.829	C	2
4140	Q	GLN	76.22042528	0.858803964	13.217	7.608	C	2

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

14.         Ruan Z, Liu C, Guo Y, et al. Potential Inhibitors Targeting RNA-Dependent RNA Polymerase Activity (NSP12) of SARS-CoV-2. 2020 [cited 2020 Apr 8];Available from: https://www.preprints.org/manuscript/202003.0024/v1