nsp8 Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

Non-structural protein 8 (nsp8)– nsp8 interacts with nsp7 to form a hexadecamer that may act as a primase.2 Nsp12-nsp7-nsp8 complex of SARS-CoV-2 is essential for viral replication and transcription and is a potential target to fight against the virus. Seven compounds (Saquinavir, Tipranavir, Lonafarnib, Tegobuvir, Olysio, Filibuvir, and Cepharanthine) were found to interact well with nsp12-nsp7-nsp8 indicating potential as drug candidates.14

Interaction of the RNA-dependent RNA polymerase (gray) with nsp7 (cyan) and nsp8

Dynamics Data for Interaction

nsp8 3,339 Sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
3943AALA46.87032046-0.0847303976.8875.449H2
3944IILE8.565438754-1.3161390184.4964.787H2
3945AALA42.64749925-0.220483815.9364.718C1
3946SSER77.448337830.8982783615.0085.148C1
3947EGLU62.413896380.4149576985.6247.207C2
3948FPHE71.758011380.715348236.3047.398C4
3949SSER48.21802935-0.041404846.465.885C3
3950SSER12.42887092-1.1919390875.4014.578H3
3951LLEU60.886492960.36585546.2586.011H6
3952PPRO57.741838870.2647624337.0775.386H3
3953SSER77.088948790.8867248795.6543.628H2
3954YTYR51.841868820.075092774.9284.148H2
3955AALA47.46930219-0.0654745946.2984.503H4
3956AALA24.79784367-0.7943067486.6383.998H3
3957FPHE2.425876011-1.5135110025.4583.317H3
3958AALA8.625336927-1.3142134375.9264.028H3
3959TTHR14.07607068-1.1389856277.1424.583H4
3960AALA99.610661871.6107430837.2294.108H4
3961QGLN32.34501348-0.5516836276.9224.662H9
3962EGLU8.924827793-1.3045855367.7825.518H8
3963AALA35.93890386-0.4361488078.6415.269H7
3964YTYR80.652890091.0012969098.6515.863H15
3965EGLU57.382449840.2532089518.9326.318H9
3966QGLN8.77508236-1.3093994879.7116.745H11
3967AALA79.484875710.96374809210.1466.669H13
3968VVAL34.14195867-0.49391621710.297.421H10
3969AALA19.76639712-0.95605549610.6627.811H10
3970NASN41.15004492-0.26862331911.2157.847H11
3971GGLY82.689427971.0667666411.6988.39H11
3972DASP32.10542078-0.55938594811.5297.741C15
3973SSER48.15813118-0.0433304212.388.487H12
3974EGLU8.6852351-1.31228785711.658.865H11
3975VVAL8.6852351-1.31228785711.6048.403H11
3976VVAL33.6328242-0.5102836510.6657.307H12
3977LLEU63.372267150.4457669849.6167.129H15
3978KLYS83.617849661.0966131359.3517.799H13
3979KLYS12.51871818-1.1890507169.326.771H11
3980LLEU79.784366580.9733759948.3755.657H14
3981KLYS18.62833184-0.9926415227.4486.187H12
3982KLYS77.029050610.8847992997.4656.423H12
3983SSER8.595387841-1.3151762287.4474.828H9
3984LLEU8.565438754-1.3161390186.1794.243H9
3985NASN99.640610961.6117058735.6344.764H6
3986VVAL31.68613357-0.5728650116.064.601H5
3987AALA99.880203651.6194081945.4753.315H2
3988KLYS99.640610961.6117058734.4114.011H2
3989SSER65.798143160.5237529874.6573.594H2
3990EGLU43.78556454-0.18389778453.624H0
3991FPHE56.903264450.2378043083.8632.952H0
3992DASP53.00988320.1126415873.6023.105H0
3993RARG69.332135370.6373622264.7034.446H0
3994DASP62.683438160.423622815.0133.612H1
3995AALA37.88559449-0.3735674463.9793.355H3
3996AALA66.636717580.5507111125.1023.815H4
3997MMET8.565438754-1.3161390186.294.875H7
3998QGLN61.575321950.3879995745.5884.966H10
3999RARG65.109314170.5016088136.0395.039H12
4000KLYS99.760407311.6155570347.7425.615H12
4001LLEU91.075172211.3363478867.795.64H14
4002EGLU51.093141660.0510230166.9535.556H16
4003KLYS17.66996107-1.0234508088.9436.164H17
4004MMET95.53758611.47980362110.0826.541H18
4005AALA99.251272841.5991896019.0296.35H20
4006DASP49.865229110.0115486199.9736.4H21
4007QGLN63.282419890.44287861312.2646.815H21
4008AALA99.850254571.61844540412.0067.196H22
4009MMET52.650494160.10108810511.1697.509H22
4010TTHR65.289008690.50738555413.4837.362H22
4011QGLN33.12368973-0.52665108314.868.077H24
4012MMET99.970050911.62229656513.2248.173H24
4013YTYR99.221323751.59822681113.7918.67H26
4014KLYS95.98682241.49424547315.9758.867T26
4015QGLN32.82419886-0.53627898416.5639.085T25
4016AALA95.657382451.48365478116.6628.992T23
4017RARG99.730458221.61459424417.9039.257T23
4018SSER11.62024558-1.21793442119.0359.475C23
4019EGLU36.98712189-0.40245115120.1919.906C22
4020DASP73.614854750.7750412221.0199.96C21
4021KLYS47.46930219-0.06547459419.57810.657C20
4022RARG28.75112309-0.66721844718.39610.707H18
4023AALA47.73884397-0.05680948215.9968.513H17
4024KLYS99.490865531.60689192213.968.253H20
4025VVAL51.063192570.05006022613.7128.445H21
4026TTHR13.44713986-1.15920422113.6247.523H17
4027SSER98.802036541.58474774911.036.13H18
4028AALA79.125486670.952194619.7966.255H18
4029MMET65.70829590.52086461710.9627.204H7
4030QGLN46.7804732-0.0876187689.5935.603H8
4031TTHR46.96016771-0.0818420277.2674.608H5
4032MMET51.632225220.0683532398.3996.06H5
4033LLEU1001.6232593559.6386.039H4
4034FPHE95.447738841.476915256.9774.346H4
4035TTHR8.595387841-1.3151762287.214.722H3
4036MMET99.970050911.62229656510.1536.366H6
4037LLEU61.545372870.38703678410.135.435H5
4038RARG54.986522910.1761857389.0795.233H6
4039KLYS69.332135370.63736222611.5466.966H7
4040LLEU84.276729561.11779451914.0017.439H9
4041DASP99.850254571.61844540413.1326.84C8
4042NASN46.18149146-0.10687457112.4876.253C8
4043DASP37.25666367-0.3937860412.2666.62H8
4044AALA23.36028751-0.84052067611.2085.554H9
4045LLEU62.443845460.41592048910.3574.729H10
4046NASN68.403713690.60751573110.5625.409H10
4047NASN10.6918239-1.2477809169.8395.216H9
4048IILE74.034141960.7885202828.4823.692H8
4049IILE37.52620545-0.3851209288.8614.065H5
4050NASN33.87241689-0.5025813299.2295.211H7
4051NASN46.81042228-0.0866559777.574.063H5
4052AALA1001.6232593556.7323.397H4
4053RARG45.61245882-0.1251675848.3925.388H5
4054DASP32.70440252-0.5401301458.0945.006H5
4055GGLY95.956873321.4932826836.3764.019C3
4056CCYS47.82869122-0.0539211125.1333.394C2
4057VVAL66.696615750.5526366925.0932.964E0
4058PPRO99.970050911.6222965655.792.642E0
4059LLEU94.758909851.4547710766.4433.399E2
4060NASN21.26385145-0.9079159889.1264.033E5
4061IILE19.70649895-0.95798107610.7073.724E7
4062IILE78.286912250.92523648613.9624.941C8
4063PPRO96.016771491.49520826316.7365.595C7
4064LLEU32.49475891-0.54686967619.5037.03C6
4065TTHR15.99281222-1.07736705719.1247.366C5
4066TTHR10.87151842-1.24200417516.8717.657C5
4067AALA54.088050310.14730203313.0276.473C2
4068AALA10.06289308-1.2679995112.8585.112E10
4069KLYS73.884396530.78370633210.8174.843E6
4070LLEU99.640610961.6117058738.0323.049E6
4071MMET8.715184187-1.3113250676.2453.609E4
4072VVAL47.73884397-0.0568094823.8812.752E0
4073VVAL79.424977540.9618225123.553.407E4
4074IILE35.8490566-0.4390371785.1783.781E8
4075PPRO76.010781670.8520644337.0554.657C6
4076DASP93.740640911.42203621110.4145.967H14
4077YTYR9.104522312-1.29880879511.4736.939H13
4078NASN2.156334232-1.52217611312.336.86H7
4079TTHR24.13896376-0.8154881329.5815.084H8
4080YTYR45.79215334-0.1193908437.9815.044H12
4081KLYS9.254267745-1.29399484410.1486.813H9
4082NASN8.116202456-1.3305808710.3945.873H7
4083TTHR9.224318658-1.2949576347.4263.983E5
4084CCYS9.314165918-1.2920692646.2174.319E7
4085DASP22.91105121-0.8549625297.9484.752E5
4086GGLY28.12219227-0.687437046.8174.525T3
4087TTHR2.096436059-1.5241016934.0944.058T2
4088TTHR12.87810722-1.1774972344.0293.804E2
4089FPHE8.924827793-1.3045855364.2142.919E2
4090TTHR37.8556454-0.3745302377.5634.015E2
4091YTYR96.166516921.5000222148.8593.248E4
4092AALA79.994010180.98011552512.9374.548T7
4093SSER8.595387841-1.31517622814.5665.392T4
4094AALA38.87391435-0.34179537112.1184.458E4
4095LLEU32.10542078-0.5593859488.5764.211E2
4096WTRP95.747229711.4865431526.7953.344E3
4097EGLU8.595387841-1.3151762285.4343.673E1
4098IILE89.817310571.2959106994.6913.495E3
4099QGLN25.48667266-0.7721625757.5384.751E4
4100QGLN9.224318658-1.2949576348.1455.222E7
4101VVAL39.95208146-0.3071349257.6024.667E13
4102VVAL9.284216831-1.2930320548.9985.441E13
4103DASP72.177298590.72882729210.2086.245E16
4104AALA35.10032944-0.46310693210.0976.277T14
4105DASP94.339622641.44129201412.967.69T11
4106SSER8.984725966-1.30265995512.1586.795E9
4107KLYS32.70440252-0.54013014513.9787.9E11
4108IILE31.74603175-0.5709394312.6097.064E12
4109VVAL64.959568730.49679486211.7346.326C16
4110QGLN29.43995208-0.64507427311.4366.859T9
4111LLEU33.15363881-0.5256882939.3265.291T6
4112SSER32.0754717-0.56034873812.0046.948T8
4113EGLU51.123090750.05198580613.9547.564T15
4114IILE31.14705001-0.59019523412.0696.108C15
4115SSER14.85474693-1.11395308314.3457.894T12
4116MMET8.595387841-1.31517622816.3119.07T10
4117DASP32.46480982-0.54783246618.9379.783T12
4118NASN43.00688829-0.20893032817.4048.814T16
4119SSER9.493860437-1.28629252315.7198.315C14
4120PPRO8.445642408-1.31999017817.0589.475C14
4121NASN53.638814020.1328601817.3969.472C13
4122LLEU62.533692720.41880885914.3337.546C15
4123AALA25.63641809-0.76734862412.8937.246C14
4124WTRP95.53758611.47980362110.1326.736C15
4125PPRO99.610661871.6107430837.6465.121C13
4126LLEU94.908655291.4595850276.9945.031E17
4127IILE14.67505241-1.1197298245.3984.038E12
4128VVAL30.57801737-0.6084882474.6663.256E6
4129TTHR19.8262953-0.9541299166.3233.838E5
4130AALA23.51003294-0.8357067266.2653.148E6
4131LLEU8.565438754-1.3161390188.5144.478E5
4132RARG99.520814621.6078547139.9385.262E4
4133AALA10.21263851-1.26318555910.5914.258C3
4134NASN8.595387841-1.31517622814.1576.194C1
4135SSER43.24648098-0.20122800713.7636.004C1
4136AALA41.26984127-0.26477215816.9877.27C2
4137VVAL60.34740940.34852517714.8277.047C2
4138KLYS65.408805030.51123671513.8418.623C2
4139LLEU76.190476190.85784117413.479.829C2
4140QGLN76.220425280.85880396413.2177.608C2

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

14.         Ruan Z, Liu C, Guo Y, et al. Potential Inhibitors Targeting RNA-Dependent RNA Polymerase Activity (NSP12) of SARS-CoV-2. 2020 [cited 2020 Apr 8];Available from: https://www.preprints.org/manuscript/202003.0024/v1