nsp7 Google Drive Data

Non-structural protein 7 (nsp7)– nsp7 interacts with nsp8 to form a hexadecamer that may act as a primase.² Nsp12-nsp7-nsp8 complex of SARS-CoV-2 is essential for viral replication and transcription and is a potential target to fight against the virus. Seven compounds (Saquinavir, Tipranavir, Lonafarnib, Tegobuvir, Olysio, Filibuvir, and Cepharanthine) were found to interact well with nsp12-nsp7-nsp8 indicating potential as drug candidates.¹⁴

Dynamics Data for Interaction

nsp7 3,256 Sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
3860	S	SER	96.13022113	1.439375744	3.704	4.007	H	0
3861	K	LYS	99.10933661	1.532222699	3.058	3.655	H	3
3862	M	MET	9.459459459	-1.261800609	2.464	2.507	H	5
3863	S	SER	24.35503686	-0.797565836	2.83	3.09	H	7
3864	D	ASP	94.74815725	1.396302414	2.883	3.176	H	8
3865	V	VAL	37.16216216	-0.398419649	2.233	2.316	H	8
3866	K	LYS	99.81572482	1.554237956	2.169	2.31	H	7
3867	C	CYS	96.28378378	1.444161669	2.663	3.079	H	9
3868	T	THR	67.22972973	0.538664565	2.405	2.687	H	8
3869	S	SER	33.78378378	-0.50371001	1.636	1.899	H	9
3870	V	VAL	99.87714988	1.556152326	2.006	2.679	H	5
3871	V	VAL	99.81572482	1.554237956	2.322	2.948	H	10
3872	L	LEU	96.43734644	1.448947595	1.808	2.143	H	6
3873	L	LEU	76.41277641	0.82486291	1.516	1.995	H	6
3874	S	SER	33.47665848	-0.513281861	2.223	2.634	H	7
3875	V	VAL	33.72235872	-0.50562438	2.19	2.628	H	6
3876	L	LEU	99.93857494	1.558066696	1.489	1.54	H	8
3877	Q	GLN	46.22235872	-0.116050044	1.978	2.472	H	5
3878	Q	GLN	33.72235872	-0.50562438	2.491	3.257	H	8
3879	L	LEU	62.71498771	0.397958355	1.968	2.326	H	3
3880	R	ARG	9.520884521	-1.259886239	1.779	3.203	T	2
3881	V	VAL	65.14127764	0.473575978	1.161	1.419	T	0
3882	E	GLU	55.8968059	0.185463262	2.06	2.23	T	1
3883	S	SER	31.23464373	-0.583156373	2.471	1.751	T	1
3884	S	SER	9.551597052	-1.258929054	1.923	1.852	H	2
3885	S	SER	95.36240786	1.415446116	2.352	2.509	H	4
3886	K	LYS	50.98280098	0.032313646	2.386	3.257	H	4
3887	L	LEU	22.26658477	-0.862654422	1.532	1.534	H	3
3888	W	TRP	80.12899263	0.940682307	1.407	2.157	H	2
3889	A	ALA	73.74078624	0.741587806	1.958	2.097	H	5
3890	Q	GLN	8.814496314	-1.281901496	1.53	2.14	H	3
3891	C	CYS	80.0982801	0.939725122	0.856	0.93	H	0
3892	V	VAL	87.5	1.170406731	1.548	1.966	H	1
3893	Q	GLN	9.398034398	-1.26371498	1.774	2.331	H	2
3894	L	LEU	72.78869779	0.711915068	1.118	1.519	H	1
3895	H	HIS	100	1.559981067	1.373	1.666	H	2
3896	N	ASN	100	1.559981067	1.92	2.132	H	3
3897	D	ASP	36.73218673	-0.41182024	1.583	1.564	H	0
3898	I	ILE	96.19164619	1.441290114	1.318	1.403	H	1
3899	L	LEU	66.06265356	0.502291531	2.019	2.52	H	3
3900	L	LEU	42.87469287	-0.22038322	2.119	2.382	H	3
3901	A	ALA	36.64004914	-0.414691795	1.662	1.777	C	2
3902	K	LYS	8.968058968	-1.277115571	1.89	2.985	C	1
3903	D	ASP	94.502457	1.388644934	1.665	2.14	H	0
3904	T	THR	9.152334152	-1.27137246	1.792	2.304	H	1
3905	T	THR	10.68796069	-1.223513205	2.015	2.499	H	3
3906	E	GLU	54.11547912	0.129946527	1.747	2.074	H	2
3907	A	ALA	74.23218673	0.756902767	1.344	1.636	H	1
3908	F	PHE	46.22235872	-0.116050044	1.69	2.362	H	3
3909	E	GLU	74.84643735	0.776046469	1.598	2.145	H	3
3910	K	LYS	52.48771499	0.079215716	1.155	1.731	H	2
3911	M	MET	8.906633907	-1.279029941	1.361	1.904	H	3
3912	V	VAL	37.07002457	-0.401291204	1.864	2.234	H	5
3913	S	SER	32.06388206	-0.557312375	1.453	1.606	H	1
3914	L	LEU	83.75307125	1.053630149	1.03	1.288	H	2
3915	L	LEU	68.7039312	0.584609449	1.72	2.283	H	3
3916	S	SER	13.11425061	-1.147895582	1.978	2.035	H	3
3917	V	VAL	21.92874693	-0.873183459	1.556	1.544	H	1
3918	L	LEU	62.56142506	0.393172429	1.637	2.24	H	3
3919	L	LEU	43.18181818	-0.210811369	2.089	2.814	H	5
3920	S	SER	44.96314496	-0.155294633	2.008	2.125	H	2
3921	M	MET	8.968058968	-1.277115571	2.204	1.832	H	2
3922	Q	GLN	11.21007371	-1.207241059	2.549	2.885	H	3
3923	G	GLY	32.89312039	-0.531468378	2.342	2.244	C	0
3924	A	ALA	40.97051597	-0.279728696	2.773	2.915	H	0
3925	V	VAL	49.38574939	-0.017459979	2.988	3.598	H	0
3926	D	ASP	71.22235872	0.663098628	2.247	2.408	H	0
3927	I	ILE	21.92874693	-0.873183459	1.933	2.203	H	0
3928	N	ASN	12.19287469	-1.176611135	2.535	2.913	H	0
3929	K	LYS	4.699017199	-1.4101643	2.602	3.342	H	0
3930	L	LEU	67.90540541	0.559722637	2.569	3.17	H	0
3931	C	CYS	36.82432432	-0.408948685	3.239	2.921	H	0
3932	E	GLU	30.0982801	-0.618572222	3.817	3.176	H	0
3933	E	GLU	9.459459459	-1.261800609	4.019	4.028	H	1
3934	M	MET	8.814496314	-1.281901496	4.447	4.478	H	3
3935	L	LEU	29.94471744	-0.623358147	7.291	5.38	T	2
3936	D	ASP	36.60933661	-0.415648981	9.137	6.706	T	2
3937	N	ASN	28.40909091	-0.671217402	6.913	6.624	T	5
3938	R	ARG	10.16584767	-1.239785352	5.775	6.041	T	3
3939	A	ALA	12.96068796	-1.152681508	6.697	7.252	C	3
3940	T	THR	11.85503686	-1.187140171	8.121	8.213	C	4
3941	L	LEU	96.4987715	1.450861965	9.933	7.431	C	2
3942	Q	GLN	99.69287469	1.550409216	11.133	9.663	C	1

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

14.         Ruan Z, Liu C, Guo Y, et al. Potential Inhibitors Targeting RNA-Dependent RNA Polymerase Activity (NSP12) of SARS-CoV-2. 2020 [cited 2020 Apr 8];Available from: https://www.preprints.org/manuscript/202003.0024/v1