nsp6 Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

Non-structural protein 6 (nsp6)- nsp6 interacts with nsp3 and nsp4 to induce double-membrane vesicles.12 Nsp6 protein also interacts with the Sigma receptor, which is thought to regulate ER stress response.8 It additionally blocks ER-induced autophagosome/autolysosome vesicle that restricts viral production and, in SARS-CoV, it leads to the generation of small autophagosome vesicles thereby limiting their expansion.13

nsp6 2,558 Sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
3570SSER51.016419080.57725637810.2466.871C0
3571AALA2.619233776-1.1124696337.2934.303C0
3572VVAL33.18999218-0.0451305857.5664.422C0
3573KLYS14.81626271-0.6866259215.9274.442H0
3574RARG22.43940579-0.4204736016.0754.058H0
3575TTHR2.658326818-1.1111047495.9992.445H0
3576IILE6.52853792-0.9759812634.3022.123H0
3577KLYS29.63252541-0.1693350015.2482.899H0
3578GGLY57.193119620.7929080027.2731.959H0
3579TTHR53.987490230.6809875399.2362.179C0
3580HHIS11.14151681-0.8149249885.8631.781H0
3581HHIS41.712275220.252414063.4531.641H0
3582WTRP60.437842060.9061933485.291.896H0
3583LLEU33.5809226-0.0314817484.0981.546H0
3584LLEU16.8881939-0.6142870851.7081.254H0
3585LLEU11.25879593-0.8108303372.6071.885H0
3586TTHR41.751368260.2537789435.5731.511C0
3587IILE2.619233776-1.1124696335.8251.348H0
3588LLEU11.96247068-0.786262432.3341.339H0
3589TTHR11.02423769-0.8190196393.0151.86H0
3590SSER11.8842846-0.7889921984.2261.519H0
3591LLEU11.18060985-0.8135601044.8031.781H0
3592LLEU12.93979672-0.7521403384.852.131H0
3593VVAL3.088350274-1.0960910284.9632.164H0
3594LLEU13.33072713-0.7384915015.1492.074H0
3595VVAL11.14151681-0.8149249884.621.968H0
3596QGLN42.25957780.2715224314.9222.727H0
3597SSER11.14151681-0.8149249884.3482.654T0
3598TTHR41.712275220.252414065.5363.88T0
3599QGLN12.11884285-0.7808028957.244.099T0
3600WTRP29.82799062-0.1625105837.0094.087T1
3601SSER13.17435496-0.7439510368.4033.888C1
3602LLEU40.734949180.2182919677.5144.755C1
3603FPHE52.189210320.6182028895.6695.404C1
3604FPHE34.519155590.001275465.464.208C0
3605FPHE11.37607506-0.8067356864.3683.957C0
3606LLEU33.62001564-0.0301168655.6644.082C0
3607YTYR11.14151681-0.8149249883.9234.183C1
3608EGLU40.695856140.2169270844.7193.892C1
3609NASN24.94136044-0.3331210444.33.631C0
3610AALA11.33698202-0.8081005692.9572.858C0
3611FPHE19.50742768-0.5228398782.082.09C0
3612LLEU12.47068022-0.7685189422.3792.376T0
3613PPRO39.874902270.1882645263.0232.379T0
3614FPHE15.87177482-0.6497740612.0361.992T0
3615AALA3.205629398-1.0919963773.3511.626T0
3616MMET2.931978108-1.1015505634.4511.588C0
3617GGLY12.00156372-0.7848975463.3651.873C0
3618IILE12.97888976-0.7507754543.3541.637C0
3619IILE3.909304144-1.0674284712.321.517C0
3620AALA15.51993745-0.6620580143.6131.184H0
3621MMET3.244722439-1.0906314945.3361.048H0
3622SSER32.56450352-0.0669687244.2421.316H0
3623AALA44.096950740.3356719654.261.324H0
3624FPHE37.255668490.0968173194.2821.545H0
3625AALA27.24784988-0.2525929064.6611.286H1
3626MMET84.71462081.7537861214.0431.988C2
3627MMET5.590304926-1.0087384724.1522.046C1
3628FPHE10.00781861-0.8545066153.2291.985C0
3629VVAL64.464425331.0467763692.9152.169C0
3630KLYS99.061767012.2546984365.7512.704C0
3631HHIS99.100860052.256063325.3332.274C0
3632KLYS98.749022672.2437793673.952.722C0
3633HHIS60.359655980.9034635814.272.538C0
3634AALA11.57154027-0.7999112675.7482.598C1
3635FPHE79.90617671.5859054275.0682.676E1
3636LLEU74.003127441.3798079892.8422.01C0
3637CCYS11.14151681-0.8149249883.1131.872C0
3638LLEU44.839718530.3616047553.0591.709E0
3639FPHE78.928850661.5517833353.2972.321E0
3640LLEU78.264268961.5285803123.71.809E0
3641LLEU55.394839720.7301233524.5691.681T0
3642PPRO99.648162632.2751716924.9091.475T0
3643SSER21.89210321-0.4395819724.9521.486T0
3644LLEU52.07193120.6141082385.4311.577T0
3645AALA11.14151681-0.8149249886.6151.085T0
3646TTHR29.20250195-0.1843487225.3311.482T0
3647VVAL13.6825645-0.7262075484.781.754T0
3648AALA23.37763878-0.3877163923.1860.981C0
3649YTYR60.75058640.9171124183.9431.254C0
3650FPHE11.18060985-0.8135601044.41.618C0
3651NASN99.061767012.2546984367.1721.903C0
3652MMET11.21970289-0.812195224.8021.734C0
3653VVAL65.715402661.0904526474.4252.049C0
3654YTYR42.689601250.2865361525.6342.494C0
3655MMET11.14151681-0.8149249889.7143.15C1
3656PPRO42.533229090.28107661712.7392.665C2
3657AALA11.18060985-0.81356010411.9683.012C1
3658SSER11.14151681-0.81492498812.2383.093C2
3659WTRP11.25879593-0.81083033710.8682.396H2
3660VVAL13.40891321-0.7357617347.2962.255H2
3661MMET11.14151681-0.8149249884.7912.934H0
3662RARG11.14151681-0.8149249884.9682.999H0
3663IILE11.64972635-0.79718153.7522.308H0
3664MMET12.62705238-0.7630594073.3582.294H0
3665TTHR11.18060985-0.8135601043.4742C0
3666WTRP29.20250195-0.1843487223.9732.645C0
3667LLEU29.74980453-0.165240356.6572.339T0
3668DASP34.44096951-0.0014543074.7841.811T0
3669MMET2.619233776-1.1124696336.9762.678T0
3670VVAL3.909304144-1.0674284715.4921.959T0
3671DASP11.18060985-0.8135601043.9952.172C0
3672TTHR13.13526192-0.7453159196.9842.527C0
3673SSER25.13682565-0.3262966266.372.08C0
3674LLEU12.39249414-0.771248715.3132.464C0
3675SSER11.18060985-0.8135601046.4533.977C0
3676GGLY11.21970289-0.812195226.4862.245C0
3677FPHE2.775605942-1.1070100986.2363.054C1
3678KLYS2.619233776-1.1124696335.1232.693C2
3679LLEU37.45113370.1036417375.1092.816C1
3680KLYS11.18060985-0.8135601045.0812.671H0
3681DASP42.533229090.2810766174.1881.673H0
3682CCYS11.14151681-0.8149249884.5231.959H0
3683VVAL13.29163409-0.7398563855.5372.227H0
3684MMET24.62861611-0.3440401146.6441.966H0
3685YTYR42.181391710.2687926646.2992.295H0
3686AALA11.68881939-0.7958166166.9221.427H0
3687SSER41.634089130.2496842922.1341.216C0
3688AALA11.10242377-0.8162898713.8441.466C0
3689VVAL6.489444879-0.9773461474.1462.198T0
3690VVAL14.07349492-0.7125587111.8961.971T0
3691LLEU46.79437060.429848941.271.746T0
3692LLEU41.79046130.2551438270.9671.745T0
3693IILE12.23612197-0.7767082441.1851.73C0
3694LLEU12.23612197-0.7767082441.3381.493C0
3695MMET15.91086787-0.6484091771.8912.008C0
3696TTHR46.168881940.4080108012.7681.372C0
3697AALA11.33698202-0.8081005693.6421.623C1
3698RARG78.498827211.5367696144.0362.816C1
3699TTHR11.14151681-0.8149249885.1262.348C1
3700VVAL13.05707584-0.7480456875.6562.58C2
3701YTYR11.45426114-0.8040059186.6096.43C1
3702DASP11.21970289-0.812195226.7235.229C3
3703DASP11.96247068-0.786262435.0072.973C1
3704GGLY2.541047694-1.11519945.3853.765C0
3705AALA12.39249414-0.771248714.9063.681T1
3706RARG11.61063331-0.7985463833.6682.796T1
3707RARG12.50977326-0.7671540583.4593.596T1
3708VVAL34.636434710.0053701113.4073.459T1
3709WTRP11.96247068-0.786262432.9653.035C0
3710TTHR11.10242377-0.8162898712.2861.992T0
3711LLEU45.973416730.4011863822.3792.158T0
3712MMET13.33072713-0.7384915012.5192.124T0
3713NASN11.18060985-0.8135601042.9611.533T0
3714VVAL17.74824081-0.5842596443.2511.628C0
3715LLEU27.8342455-0.2321196514.1081.669C1
3716TTHR38.272087570.1323042954.2571.578C3
3717LLEU15.44175137-0.6647877824.3662.113C2
3718VVAL20.48475371-0.4887177855.2152.456C2
3719YTYR78.811571541.5476886844.2082.696C1
3720KLYS11.10242377-0.8162898713.9062.898C1
3721VVAL11.49335418-0.8026410344.7792.817C0
3722YTYR33.34636435-0.039671054.0442.48T0
3723YTYR45.035183740.3684291733.9932.216T1
3724GGLY63.291634091.0058298583.1551.813T1
3725NASN11.37607506-0.8067356863.5392.279T0
3726AALA10.67240031-0.8313035923.4841.96C0
3727LLEU31.0398749-0.1201991887.7773.291C0
3728DASP11.06333073-0.8176547557.5282.508C0
3729QGLN11.14151681-0.8149249887.9372.704C1
3730AALA11.80609851-0.7917219656.812.257C2
3731IILE55.199374510.7232989335.1272.794C1
3732SSER11.64972635-0.79718156.5432.703C1
3733MMET11.10242377-0.8162898716.2172.84T1
3734WTRP11.18060985-0.8135601045.4673.821T0
3735AALA11.14151681-0.8149249885.4642.285T0
3736LLEU41.477716970.2442247575.5222.782T0
3737IILE4.652071931-1.0414956815.5172.997C1
3738IILE13.21344801-0.7425861524.6362.237C1
3739SSER12.50977326-0.7671540583.7621.761C1
3740VVAL14.03440188-0.7139235946.5422.97C2
3741TTHR41.868647380.2578735946.513.195C1
3742SSER46.012509770.4025512664.7932.416C1
3743NASN13.99530884-0.7152884787.1413.525C2
3744YTYR42.650508210.2851712686.1234.687C1
3745SSER20.05473026-0.5037315069.4023.722T0
3746GGLY34.40187647-0.0028191915.9392.572T0
3747VVAL11.92337764-0.7876273144.6342.84T1
3748VVAL41.868647380.2578735944.62.715T1
3749TTHR29.241595-0.1829838384.2672.341C0
3750TTHR29.31978108-0.1802540713.0012.093C0
3751VVAL13.87802971-0.7193831293.9222.196C1
3752MMET11.21970289-0.812195224.0492.303C2
3753FPHE11.76700547-0.7930868494.1911.972C4
3754LLEU45.504300230.3848077786.1973.055C3
3755AALA59.968725570.8898147447.2083.042C4
3756RARG22.32212666-0.4245682526.6312.713C5
3757GGLY2.853792025-1.104280337.7022.269T5
3758IILE11.8842846-0.7889921986.2552.003T3
3759VVAL49.765441750.53358017.1612.27T3
3760FPHE17.3573104-0.5979084818.3443.321T3
3761MMET24.00312744-0.3658782536.6282.528C2
3762CCYS11.21970289-0.812195224.3811.711T2
3763VVAL13.40891321-0.7357617344.1572.271T2
3764EGLU11.10242377-0.8162898718.1272.615T2
3765YTYR42.4550430.278346859.1053.546T2
3766CCYS2.619233776-1.11246963310.6552.876C2
3767PPRO67.630961691.15733194810.8692.986C2
3768IILE2.853792025-1.1042803310.8712.731C2
3769FPHE27.24784988-0.25259290611.6432.268C2
3770FPHE32.29085223-0.0765229114.5073.939C1
3771IILE11.4151681-0.80537080215.1344.643T0
3772TTHR11.4151681-0.80537080212.9743.346T1
3773GGLY11.10242377-0.81628987110.0282.945T1
3774NASN11.57154027-0.7999112678.7452.808T1
3775TTHR11.18060985-0.8135601046.5382.591C2
3776LLEU11.45426114-0.8040059185.562.071C2
3777QGLN11.10242377-0.8162898716.6231.874C2
3778CCYS14.30805317-0.7043694096.3741.548C2
3779IILE44.253322910.34113152.9941.514T1
3780MMET34.558248630.0026403444.0851.288T0
3781LLEU61.063330730.9280314873.4811.15T0
3782VVAL16.37998436-0.6320305731.2461.078T0
3783YTYR99.33541832.2642526221.6311.31C0
3784CCYS11.14151681-0.8149249883.7440.988C0
3785FPHE27.71696638-0.2362143025.1111.424C0
3786LLEU68.921032061.2023731095.0011.751C0
3787GGLY99.843627832.281996112.631.542C0
3788YTYR64.190774041.0372221832.0921.587C1
3789FPHE26.66145426-0.2730661621.4481.267C1
3790CCYS46.989835810.4366733583.8371.301T0
3791TTHR36.473807660.0695196454.1171.799T1
3792CCYS84.323690381.7401372843.3971.691T1
3793YTYR76.465989051.4657956622.7491.412T0
3794FPHE45.230648940.3752535923.1712.36C1
3795GGLY99.960906962.2860907613.2422.225C2
3796LLEU31.35261923-0.1092801194.8162.774C2
3797FPHE59.030492570.8570575353.5582.032C1
3798CCYS11.18060985-0.8135601044.4421.961C0
3799LLEU57.114933540.7901782344.1211.38C0
3800LLEU48.318999220.4830794049.1252.216C0
3801NASN99.960906962.2860907617.2551.851C0
3802RARG49.726348710.5322152175.6782.205C0
3803YTYR11.18060985-0.8135601042.7911.87C0
3804FPHE57.349491790.7983675373.7531.912C0
3805RARG60.789679440.9184773014.4012.605C0
3806LLEU16.45817045-0.6293008066.0392.666C0
3807TTHR27.79515246-0.2334845355.5172.525C0
3808LLEU38.819390150.1514126666.1683.213C1
3809GGLY99.960906962.2860907616.2092.85C1
3810VVAL93.979671622.0772635567.2343.155C0
3811YTYR99.882720882.2833609947.6352.773C0
3812DASP77.091477721.4876338016.2262.681C0
3813YTYR54.222048480.6891768415.7533.988C0
3814LLEU11.25879593-0.8108303377.0913.177C0
3815VVAL81.899921811.6555144956.0492.279C0
3816SSER99.882720882.2833609945.4881.775C0
3817TTHR41.946833460.2606033625.532.884C0
3818QGLN60.476935110.9075582326.7323.442C1
3819EGLU99.257232212.2615228555.0692.641C1
3820FPHE77.990617671.5190261264.8133.719C0
3821RARG61.297888980.9362207898.2924.863C0
3822YTYR70.132916341.2446845048.383.421C0
3823MMET98.631743552.2396847168.835.446C1
3824NASN30.25801407-0.1474968626.4133.215C1
3825SSER12.00156372-0.7848975468.6673.297C2
3826QGLN11.18060985-0.8135601048.2392.992C1
3827GGLY66.966379981.1341289257.9493.488C1
3828LLEU97.498045352.2001030888.2394.372C2
3829LLEU11.76700547-0.7930868497.8674.265C2
3830PPRO30.33620016-0.1447670959.174.309C3
3831PPRO99.960906962.28609076112.7355.981C3
3832KLYS27.36512901-0.24849825513.0785.989C4
3833NASN52.892885070.64277079611.9286.237C4
3834SSER60.75058640.9171124188.2625.058C3
3835IILE11.06333073-0.8176547556.9693.723C3
3836DASP49.452697420.5226610317.2332.994C1
3837AALA73.768569191.3716186876.9352.257C1
3838FPHE11.84519156-0.7903570817.6832.328C1
3839KLYS11.25879593-0.8108303378.5243.567C1
3840LLEU99.296325252.2628877386.1732.958C0
3841NASN61.806098510.9539642775.5432.268C0
3842IILE13.25254105-0.7412212685.0962.254C0
3843KLYS96.090695862.1509672764.4832.332C0
3844LLEU99.413604382.2669823896.6262.137C0
3845LLEU35.8483190.0476815065.3421.945C0
3846GGLY99.960906962.2860907614.9531.731C1
3847VVAL7.388584832-0.9459538225.1172.141C1
3848GGLY99.960906962.2860907614.7071.973C0
3849GGLY99.882720882.2833609944.522.319C0
3850KLYS12.74433151-0.7589647568.8493.635C1
3851PPRO60.75058640.91711241810.964.138C2
3852CCYS45.699765440.39163219615.6664.218C2
3853IILE98.592650512.23831983219.6766.251C2
3854KLYS81.118060991.62821682118.8197.073C2
3855VVAL67.396403441.14914264514.6934.974C2
3856AALA42.963252540.29609033812.4473.502C1
3857TTHR26.73964034-0.27033639411.5273.081C0
3858VVAL52.26739640.6209326579.3593.19C0
3859QGLN99.921813922.2847258778.0983.736C0

8.            Gordon DE, Jang GM, Bouhaddou M, et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. bioRxiv 2020;2020.03.22.002386.

12.         Angelini MM, Akhlaghpour M, Neuman BW, Buchmeier MJ. Severe Acute Respiratory Syndrome Coronavirus Nonstructural Proteins 3, 4, and 6 Induce Double-Membrane Vesicles. mBio [Internet] 2013 [cited 2020 Apr 8];4(4). Available from: https://mbio.asm.org/content/4/4/e00524-13

13.         Cottam EM, Whelband MC, Wileman T. Coronavirus NSP6 restricts autophagosome expansion. Autophagy 2014;10(8):1426–41.