nsp10 Google Drive Data

Non-structural protein 10 (nsp10)– nsp10 stimulates nsp14 3′-5′ exoribonuclease and nsp16 2′-O-methyltransferase activities. Due to its activities with nsp14 and 16 it is involved with viral mRNAs cap methylation.² Nsp9 and nsp10 interact with NKRF, an NF-КB repressor, and may cause a IL-8/IL-6 mediated chemotaxis of neutrophils and overexuberant host inflammatory response.¹⁵ Certain -N=N and -COOH based Protein-nucleic acid interface inhibitors show good binding energy with nsp10/16 protein.¹⁶

nsp10 3,344 Sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
4254	A	ALA	79.84449761	0.632911923	14.872	10.54	C	1
4255	G	GLY	99.76076555	1.196521764	11.349	8.486	C	2
4256	N	ASN	8.761961722	-1.378650531	8.261	7.622	C	2
4257	A	ALA	23.02631579	-0.974984022	6.618	7.137	C	2
4258	T	THR	95.6638756	1.080584004	4.82	5.897	C	2
4259	E	GLU	99.61124402	1.192290459	3.922	4.179	H	1
4260	V	VAL	9.300239234	-1.363417832	3.102	3.657	H	0
4261	P	PRO	14.4138756	-1.218707197	2.484	3.197	H	0
4262	A	ALA	11.48325359	-1.301640778	2.502	2.72	H	0
4263	N	ASN	83.91148325	0.748003422	2.209	2.468	H	0
4264	S	SER	79.60526316	0.626141834	1.973	1.869	H	0
4265	T	THR	9.18062201	-1.366802876	1.865	1.681	H	0
4266	V	VAL	32.47607656	-0.707565539	2.546	1.747	H	0
4267	L	LEU	99.64114833	1.19313672	2.43	1.783	H	0
4268	S	SER	62.52990431	0.14292679	2.545	1.843	H	0
4269	F	PHE	8.821770335	-1.376958009	2.674	2.236	H	1
4270	C	CYS	72.30861244	0.419654145	1.798	1.417	H	1
4271	A	ALA	58.28349282	0.022757725	2.395	1.735	H	0
4272	F	PHE	99.94019139	1.201599331	2.24	2.061	H	0
4273	A	ALA	58.10406699	0.017680158	1.795	1.398	C	0
4274	V	VAL	93.92942584	1.031500865	2.09	1.919	C	0
4275	D	ASP	99.16267943	1.179596544	1.861	1.302	H	1
4276	A	ALA	2.21291866	-1.563981695	1.181	1.12	H	1
4277	A	ALA	56.45933014	-0.028864198	1.315	1.138	H	0
4278	K	LYS	73.20574163	0.445041975	1.045	1.967	H	0
4279	A	ALA	46.86004785	-0.300513987	1.138	1.181	H	0
4280	Y	TYR	99.88038278	1.199906808	1.102	1.078	H	0
4281	K	LYS	9.419856459	-1.360032788	1.822	1.906	H	1
4282	D	ASP	74.94019139	0.494125115	2.182	1.318	H	2
4283	Y	TYR	29.48564593	-0.792191641	2.26	1.366	H	1
4284	L	LEU	13.03827751	-1.257635204	2.648	1.713	H	1
4285	A	ALA	27.78110048	-0.84042852	3.201	1.686	H	1
4286	S	SER	28.02033493	-0.833658431	3.658	1.804	C	1
4287	G	GLY	99.97009569	1.202445592	4.042	1.66	C	1
4288	G	GLY	51.25598086	-0.176113616	2.867	1.416	C	0
4289	Q	GLN	29.72488038	-0.785421553	2.589	1.934	C	0
4290	P	PRO	99.94019139	1.201599331	2.056	1.513	C	0
4291	I	ILE	25.98684211	-0.891204181	1.38	1.211	C	0
4292	T	THR	36.93181818	-0.581472646	1.377	1.875	C	0
4293	N	ASN	95.93301435	1.088200353	1.991	2.305	C	0
4294	C	CYS	99.34210526	1.18467411	0.922	0.999	C	0
4295	V	VAL	91.38755981	0.959568678	1.13	1.233	C	0
4296	K	LYS	95.99282297	1.089892875	1.225	1.891	C	0
4297	M	MET	99.88038278	1.199906808	1.164	1.294	C	0
4298	L	LEU	95.78349282	1.083969048	1.657	1.899	C	0
4299	C	CYS	22.75717703	-0.982600372	1.446	1.262	C	0
4300	T	THR	12.47009569	-1.273714164	1.885	1.418	C	0
4301	H	HIS	39.08492823	-0.520541853	2.126	1.252	C	0
4302	T	THR	33.28349282	-0.684716491	2.192	1.348	C	0
4303	G	GLY	100	1.203291853	1.503	0.982	C	0
4304	T	THR	48.23564593	-0.261585979	1.332	1.172	C	0
4305	G	GLY	99.97009569	1.202445592	0.874	0.781	C	0
4306	Q	GLN	36.18421053	-0.602629172	0.763	1.046	C	0
4307	A	ALA	99.97009569	1.202445592	0.664	0.863	E	0
4308	I	ILE	73.89354067	0.464505979	0.676	1.018	E	0
4309	T	THR	75.98684211	0.523744251	0.886	0.894	E	1
4310	V	VAL	43.86961722	-0.385140089	1.078	1.44	C	1
4311	T	THR	12.29066986	-1.27879173	1.112	1.154	C	0
4312	P	PRO	66.8062201	0.263942116	1.094	1.047	C	0
4313	E	GLU	62.73923445	0.148850617	1.21	1.08	C	0
4314	A	ALA	51.01674641	-0.182883704	1.356	0.97	C	0
4315	N	ASN	28.64832536	-0.81588695	1.461	1.269	T	0
4316	M	MET	10.16746411	-1.338876263	1.86	1.856	T	0
4317	D	ASP	48.29545455	-0.259893457	1.819	1.401	T	1
4318	Q	GLN	99.97009569	1.202445592	1.476	0.864	T	2
4319	E	GLU	32.6854067	-0.701641712	1.153	1.302	E	2
4320	S	SER	72.99641148	0.439118148	0.893	0.886	E	1
4321	F	PHE	9.748803828	-1.350723917	0.845	0.867	E	0
4322	G	GLY	100	1.203291853	0.944	0.852	T	0
4323	G	GLY	100	1.203291853	0.905	0.809	T	0
4324	A	ALA	99.70095694	1.194829242	1.064	1.047	T	0
4325	S	SER	99.97009569	1.202445592	1.369	0.882	T	0
4326	C	CYS	9.629186603	-1.354108961	1.273	0.724	C	0
4327	C	CYS	99.94019139	1.201599331	1.271	0.656	H	0
4328	L	LEU	75.71770335	0.516127901	1.259	1.073	H	0
4329	Y	TYR	99.13277512	1.178750283	0.897	0.867	H	0
4330	C	CYS	99.94019139	1.201599331	1.239	0.894	H	0
4331	R	ARG	99.97009569	1.202445592	1.684	1.217	H	1
4332	C	CYS	14.50358852	-1.216168414	1.486	1.087	H	1
4333	H	HIS	85.22727273	0.785238907	1.555	1.736	C	0
4334	I	ILE	53.19976077	-0.12110665	1.318	1.25	C	0
4335	D	ASP	10.49641148	-1.329567391	1.273	1.38	C	0
4336	H	HIS	100	1.203291853	0.999	1.038	C	0
4337	P	PRO	99.79066986	1.197368025	1.53	1.34	C	0
4338	N	ASN	24.28229665	-0.939441059	2.543	1.627	T	1
4339	P	PRO	8.911483254	-1.374419226	2.822	2.131	T	1
4340	K	LYS	8.791866029	-1.37780427	2.252	2.855	T	1
4341	G	GLY	99.91028708	1.20075307	1.854	1.546	T	2
4342	F	PHE	30.71172249	-0.757494939	2.717	2.344	C	2
4343	C	CYS	99.94019139	1.201599331	1.962	1.307	C	1
4344	D	ASP	8.791866029	-1.37780427	2.07	1.576	C	0
4345	L	LEU	42.34449761	-0.428299401	1.299	1.075	T	0
4346	K	LYS	84.92822967	0.776776297	1.498	2.055	T	0
4347	G	GLY	99.49162679	1.188905415	1.272	1.089	T	0
4348	K	LYS	77.75119617	0.573673651	0.766	1.268	T	0
4349	Y	TYR	30.2930622	-0.769342594	0.74	1.302	E	0
4350	V	VAL	99.82057416	1.198214286	0.764	0.947	E	0
4351	Q	GLN	96.23205742	1.096662964	0.925	0.893	E	0
4352	I	ILE	55.32296651	-0.061022117	1.312	0.939	E	1
4353	P	PRO	96.02272727	1.090739136	1.341	0.915	T	2
4354	T	THR	29.60526316	-0.788806597	1.674	1.447	T	1
4355	T	THR	24.07296651	-0.945364887	1.813	1.471	T	0
4356	C	CYS	34.47966507	-0.65086605	1.686	1.086	T	1
4357	A	ALA	8.911483254	-1.374419226	1.639	1.167	C	2
4358	N	ASN	9.210526316	-1.365956615	2.226	1.465	C	2
4359	D	ASP	62.88875598	0.153081922	1.926	1.067	H	2
4360	P	PRO	100	1.203291853	1.412	1.011	H	2
4361	V	VAL	65.34090909	0.222475326	1.635	1.132	H	1
4362	G	GLY	49.19258373	-0.234505627	1.946	1.083	H	1
4363	F	PHE	85.43660287	0.791162734	1.511	0.907	H	1
4364	T	THR	8.70215311	-1.380343053	1.494	1.065	H	0
4365	L	LEU	92.19497608	0.982417725	1.714	1.82	H	0
4366	K	LYS	2.781100478	-1.547902735	1.396	1.877	H	0
4367	N	ASN	82.08732057	0.696381499	1.328	0.937	C	0
4368	T	THR	35.73564593	-0.615323087	1.325	1.381	C	0
4369	V	VAL	70.06578947	0.356184568	0.955	1.162	C	0
4370	C	CYS	100	1.203291853	0.799	0.864	C	0
4371	T	THR	24.61124402	-0.930132188	1.304	1.334	C	0
4372	V	VAL	95.30502392	1.070428872	1.192	1.378	C	0
4373	C	CYS	100	1.203291853	0.995	0.933	C	0
4374	G	GLY	53.52870813	-0.111797778	0.932	0.847	C	0
4375	M	MET	9.539473684	-1.356647744	0.724	1.051	C	0
4376	W	TRP	99.94019139	1.201599331	0.902	0.804	T	0
4377	K	LYS	9.210526316	-1.365956615	1.075	1.58	T	0
4378	G	GLY	52.18301435	-0.149879524	1.335	1.035	T	0
4379	Y	TYR	44.82655502	-0.358059736	1.255	1.153	T	0
4380	G	GLY	82.11722488	0.69722776	1.038	0.988	C	0
4381	C	CYS	99.91028708	1.20075307	0.879	0.886	C	0
4382	S	SER	24.40191388	-0.936056015	1.155	1.263	C	0
4383	C	CYS	99.82057416	1.198214286	1.186	1.072	C	0
4384	D	ASP	80.74162679	0.658299753	1.971	1.736	T	0
4385	Q	GLN	8.70215311	-1.380343053	2.707	2.715	T	0
4386	L	LEU	48.50478469	-0.25396963	3.047	3.034	T	0
4387	R	ARG	48.74401914	-0.247199542	2.242	3.217	T	0
4388	E	GLU	9.868421053	-1.347338873	1.851	2.006	C	0
4389	P	PRO	22.63755981	-0.985985416	3.018	2.738	C	0
4390	M	MET	4.90430622	-1.487818203	4.429	4.934	C	1
4391	L	LEU	25.17942584	-0.914053229	5.673	5.931	C	1
4392	Q	GLN	9.210526316	-1.365956615	6.99	5.907	C	0

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

15.         Li J, Guo M, Tian X, et al. Virus-host interactome and proteomic survey of PMBCs from COVID-19 patients reveal potential virulence factors influencing SARS-CoV-2 pathogenesis. bioRxiv 2020;2020.03.31.019216.

16.         Khadim Sheikh H, Arshad T, Mohammad ZS, Arshad I, Hassan M. PROTEIN-NUCLEIC ACID INTERFACE (PNAI) INHIBITOR DRUG MOLECULES FOR SARS-COV-2. 2020 [cited 2020 Apr 9];Available from: https://chemrxiv.org/articles/PROTEIN-NUCLEIC_ACID_INTERFACE_PNAI_INHIBITOR_DRUG_MOLECULES_FOR_SARS-COV-2/12026736