nsp1 Google Drive Data

Assists in evasion from the immune system by suppressing host gene expression. Nsp1 interacts with the 40S ribosomal unit forming the nsp1-40s ribosome complex which causes endonucleolytic cleavage of the 5’UTR of host mRNAs, resulting in degradation. Viral mRNAs have a leader sequence on the 5’ end protecting them from this cleavage.² In SARS-CoV, nsp1 modulates the Calcineurin/NFAT pathway which is important for immune cell activation.³

nsp1 250 sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
1	M	MET	100	0.455425776	12.403	9.753	C	1
2	E	GLU	100	0.455425776	10.296	9.68	C	2
3	S	SER	100	0.455425776	8.218	8.128	C	2
4	L	LEU	100	0.455425776	8.929	8.448	C	2
5	V	VAL	99.2	0.429322722	7.121	6.893	C	2
6	P	PRO	14.4	-2.337600953	5.792	5.926	C	1
7	G	GLY	100	0.455425776	5.592	4.435	T	0
8	F	PHE	14.4	-2.337600953	6.295	5.595	T	0
9	N	ASN	98.4	0.403219669	6.405	4.493	T	0
10	E	GLU	100	0.455425776	4.439	4.246	T	0
11	K	LYS	100	0.455425776	4.032	3.886	C	0
12	T	THR	100	0.455425776	4.617	2.515	C	1
13	H	HIS	100	0.455425776	5.735	2.196	C	3
14	V	VAL	99.6	0.442374249	5.431	2.702	E	2
15	Q	GLN	99.6	0.442374249	5.218	3.015	E	2
16	L	LEU	100	0.455425776	4.278	2.559	E	1
17	S	SER	100	0.455425776	4.109	3.006	E	2
18	L	LEU	100	0.455425776	3.134	2.038	E	2
19	P	PRO	100	0.455425776	3.022	2.427	E	5
20	V	VAL	100	0.455425776	2.835	2.515	E	7
21	L	LEU	100	0.455425776	2.948	2.216	E	5
22	Q	GLN	100	0.455425776	3.335	2.764	T	1
23	V	VAL	100	0.455425776	2.82	2.218	T	0
24	R	ARG	93.6	0.246601347	3.682	3.146	T	1
25	D	ASP	100	0.455425776	3.517	2.509	T	2
26	V	VAL	100	0.455425776	2.761	1.733	C	1
27	L	LEU	100	0.455425776	3.026	2.937	C	0
28	V	VAL	100	0.455425776	2.595	2.161	C	0
29	R	ARG	100	0.455425776	2.212	1.858	C	0
30	G	GLY	100	0.455425776	3.093	1.746	C	2
31	F	PHE	100	0.455425776	3.4	2.063	C	8
32	G	GLY	100	0.455425776	4.402	2.812	C	10
33	D	ASP	100	0.455425776	4.939	3.466	C	8
34	S	SER	93.6	0.246601347	5.571	4.101	H	10
35	V	VAL	100	0.455425776	4.943	3.712	H	10
36	E	GLU	100	0.455425776	5.475	4.503	H	7
37	E	GLU	100	0.455425776	6.093	4.342	H	10
38	V	VAL	12.4	-2.402858586	4.769	2.995	H	10
39	L	LEU	99.2	0.429322722	4.531	2.781	H	10
40	S	SER	91.6	0.181343714	4.909	3.576	H	8
41	E	GLU	100	0.455425776	3.857	3.269	H	9
42	A	ALA	99.6	0.442374249	2.94	2.275	H	5
43	R	ARG	100	0.455425776	3.553	2.89	H	5
44	Q	GLN	14.4	-2.337600953	3.645	3.822	H	6
45	H	HIS	100	0.455425776	2.823	2.747	H	6
46	L	LEU	100	0.455425776	2.84	2.622	H	7
47	K	LYS	99.2	0.429322722	3.839	3.796	H	6
48	D	ASP	14	-2.350652479	3.393	3.649	H	6
49	G	GLY	100	0.455425776	3.171	3.18	H	6
50	T	THR	100	0.455425776	2.666	2.978	E	9
51	C	CYS	100	0.455425776	2.12	2.136	E	7
52	G	GLY	100	0.455425776	1.816	1.495	E	0
53	L	LEU	96	0.324910508	1.633	1.807	E	0
54	V	VAL	99.6	0.442374249	1.904	1.854	E	0
55	E	GLU	99.2	0.429322722	3.279	2.872	C	0
56	V	VAL	13.6	-2.363704006	4.064	2.727	C	1
57	E	GLU	99.2	0.429322722	4.449	3.613	C	2
58	K	LYS	100	0.455425776	5.243	4.453	C	2
59	G	GLY	100	0.455425776	5.855	3.443	C	3
60	V	VAL	99.6	0.442374249	4.533	2.936	G	2
61	L	LEU	100	0.455425776	2.921	2.754	G	3
62	P	PRO	94.4	0.272704401	5.28	2.942	G	3
63	Q	GLN	100	0.455425776	4.805	2.868	G	2
64	L	LEU	99.2	0.429322722	3.185	1.884	C	2
65	E	GLU	100	0.455425776	4.029	2.115	C	2
66	Q	GLN	94.4	0.272704401	4.204	3.445	C	4
67	P	PRO	100	0.455425776	3.723	2.711	C	10
68	Y	TYR	100	0.455425776	3.482	2.216	E	7
69	V	VAL	99.6	0.442374249	2.863	1.793	E	8
70	F	PHE	100	0.455425776	2.586	1.86	E	3
71	I	ILE	99.2	0.429322722	2.736	1.583	E	2
72	K	LYS	100	0.455425776	3.485	2.408	E	2
73	R	ARG	100	0.455425776	3.634	2.224	C	1
74	S	SER	99.6	0.442374249	5.23	1.987	C	0
75	D	ASP	93.6	0.246601347	6.282	3.04	C	0
76	A	ALA	100	0.455425776	8.704	3.228	C	0
77	R	ARG	14	-2.350652479	8.377	3.022	C	0
78	T	THR	14.4	-2.337600953	7.188	3.598	C	0
79	A	ALA	14.4	-2.337600953	7.081	2.87	C	0
80	P	PRO	14	-2.350652479	6.444	3.444	C	0
81	H	HIS	100	0.455425776	7.835	4.051	C	1
82	G	GLY	98.4	0.403219669	7.854	3.077	C	1
83	H	HIS	98.8	0.416271195	5.596	2.63	C	0
84	V	VAL	14	-2.350652479	4.175	2.08	E	0
85	M	MET	14	-2.350652479	4.414	2.089	E	0
86	V	VAL	95.2	0.298807454	4.023	1.397	E	0
87	E	GLU	100	0.455425776	4.224	1.768	E	1
88	L	LEU	100	0.455425776	3.776	1.936	E	2
89	V	VAL	100	0.455425776	4.265	2.449	E	5
90	A	ALA	100	0.455425776	4.563	2.737	E	8
91	E	GLU	100	0.455425776	4.878	3.084	E	7
92	L	LEU	39.6	-1.515354766	5.603	3.709	C	9
93	E	GLU	13.2	-2.376755533	5.816	3.963	C	6
94	G	GLY	100	0.455425776	4.225	3.123	C	5
95	I	ILE	94	0.259652874	5.046	4.119	C	5
96	Q	GLN	99.6	0.442374249	4.626	3.922	C	6
97	Y	TYR	86.4	0.011673866	3.777	2.983	T	7
98	G	GLY	100	0.455425776	3.24	2.773	T	7
99	R	ARG	100	0.455425776	4.368	4.195	T	6
100	S	SER	100	0.455425776	4.254	3.136	T	6
101	G	GLY	100	0.455425776	3.629	3.029	C	4
102	E	GLU	14.4	-2.337600953	3.63	2.66	C	4
103	T	THR	99.6	0.442374249	2.594	2.144	C	5
104	L	LEU	100	0.455425776	2.082	1.976	E	1
105	G	GLY	100	0.455425776	1.852	1.475	E	1
106	V	VAL	100	0.455425776	1.494	1.516	E	0
107	L	LEU	100	0.455425776	1.538	1.523	E	0
108	V	VAL	100	0.455425776	1.678	1.32	E	0
109	P	PRO	100	0.455425776	2.048	1.906	E	2
110	H	HIS	99.2	0.429322722	2.261	2.571	C	4
111	V	VAL	99.2	0.429322722	3.247	3.537	C	5
112	G	GLY	99.6	0.442374249	3.674	3.367	C	3
113	E	GLU	100	0.455425776	3.641	2.723	C	2
114	I	ILE	12.4	-2.402858586	4.589	3.838	C	3
115	P	PRO	100	0.455425776	4.153	3.247	C	7
116	V	VAL	14.4	-2.337600953	4.989	3.574	E	6
117	A	ALA	98.4	0.403219669	4.756	3.548	E	6
118	Y	TYR	100	0.455425776	4.327	3.625	E	7
119	R	ARG	100	0.455425776	4.294	2.977	E	5
120	K	LYS	14.4	-2.337600953	4.73	3.55	E	4
121	V	VAL	100	0.455425776	4.766	2.026	E	1
122	L	LEU	100	0.455425776	5.43	2.048	E	0
123	L	LEU	99.2	0.429322722	5.461	1.92	E	2
124	R	ARG	100	0.455425776	5.87	2.4	C	2
125	K	LYS	100	0.455425776	6.23	3.411	C	2
126	N	ASN	99.2	0.429322722	6.528	3.371	C	1
127	G	GLY	100	0.455425776	4.859	2.463	C	0
128	N	ASN	100	0.455425776	2.629	2.9	C	0
129	K	LYS	100	0.455425776	4.018	2.985	C	0
130	G	GLY	98.8	0.416271195	5.142	2.464	C	0
131	A	ALA	100	0.455425776	4.786	3.334	C	0
132	G	GLY	100	0.455425776	4.565	3.283	C	0
133	G	GLY	100	0.455425776	7.066	3.882	T	0
134	H	HIS	100	0.455425776	10.158	5.474	T	0
135	S	SER	99.2	0.429322722	8.491	4.808	T	0
136	Y	TYR	76.8	-0.301562777	9.578	4.689	T	0
137	G	GLY	100	0.455425776	11.549	4.329	C	0
138	A	ALA	14.4	-2.337600953	11.633	4.931	C	0
139	D	ASP	100	0.455425776	11.707	4.98	C	1
140	L	LEU	100	0.455425776	13.2	5.894	C	2
141	K	LYS	96.8	0.351013562	12.36	5.953	C	2
142	S	SER	99.2	0.429322722	13.244	4.392	C	2
143	F	PHE	13.2	-2.376755533	10.038	3.528	C	2
144	D	ASP	100	0.455425776	9.611	3.808	C	2
145	L	LEU	100	0.455425776	8.294	4.457	C	2
146	G	GLY	100	0.455425776	6.479	4.715	T	3
147	D	ASP	98.8	0.416271195	6.73	5.713	T	4
148	E	GLU	96.4	0.337962035	9.59	5.356	T	3
149	L	LEU	100	0.455425776	5.291	3.677	T	6
150	G	GLY	94.8	0.285755928	8.299	4.462	C	4
151	T	THR	100	0.455425776	10.767	4.742	C	4
152	D	ASP	100	0.455425776	7.066	3.879	C	4
153	P	PRO	99.2	0.429322722	4.575	3.915	C	2
154	Y	TYR	12.8	-2.38980706	5.264	4.089	C	3
155	E	GLU	99.6	0.442374249	3.771	3.24	H	3
156	D	ASP	100	0.455425776	3.818	3.876	H	1
157	F	PHE	14	-2.350652479	4.062	4.108	H	2
158	Q	GLN	14	-2.350652479	3.455	2.955	H	0
159	E	GLU	13.6	-2.363704006	3.943	3.091	H	1
160	N	ASN	93.2	0.233549821	4.034	3.364	H	1
161	W	TRP	100	0.455425776	5.654	4.048	H	1
162	N	ASN	100	0.455425776	6.553	3.448	C	2
163	T	THR	100	0.455425776	6.726	4.14	H	2
164	K	LYS	100	0.455425776	7.7	4.912	H	2
165	H	HIS	100	0.455425776	6.645	4.162	H	2
166	S	SER	12.8	-2.38980706	5.928	3.871	H	3
167	S	SER	95.2	0.298807454	6.244	4.62	H	3
168	G	GLY	100	0.455425776	8.119	4.558	H	2
169	V	VAL	29.2	-1.854694462	8.524	4.344	H	4
170	T	THR	13.6	-2.363704006	7.337	4.557	H	5
171	R	ARG	100	0.455425776	8.57	6.01	H	7
172	E	GLU	98.8	0.416271195	10.97	5.582	H	6
173	L	LEU	100	0.455425776	11.013	5.524	H	8
174	M	MET	12.8	-2.38980706	10.121	5.981	H	7
175	R	ARG	100	0.455425776	12.342	6.805	H	7
176	E	GLU	100	0.455425776	14.528	6.742	H	6
177	L	LEU	100	0.455425776	14.115	6.876	H	5
178	N	ASN	100	0.455425776	15.866	7.296	C	6
179	G	GLY	100	0.455425776	20.436	8.047	C	5
180	G	GLY	100	0.455425776	25.093	9.249	C	1

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

3.            Cui H, Gao Z, Liu M, et al. Structural Genomics and Interactomics of 2019 Wuhan Novel Coronavirus, SARS-CoV-2, Indicate Evolutionary Conserved Functional Regions of Viral Proteins. 2020 [cited 2020 Apr 10];Available from: https://www.preprints.org/manuscript/202002.0372/v1