nsp1 Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

Assists in evasion from the immune system by suppressing host gene expression. Nsp1 interacts with the 40S ribosomal unit forming the nsp1-40s ribosome complex which causes endonucleolytic cleavage of the 5’UTR of host mRNAs, resulting in degradation. Viral mRNAs have a leader sequence on the 5’ end protecting them from this cleavage.2 In SARS-CoV, nsp1 modulates the Calcineurin/NFAT pathway which is important for immune cell activation.3

nsp1 250 sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
1MMET1000.45542577612.4039.753C1
2EGLU1000.45542577610.2969.68C2
3SSER1000.4554257768.2188.128C2
4LLEU1000.4554257768.9298.448C2
5VVAL99.20.4293227227.1216.893C2
6PPRO14.4-2.3376009535.7925.926C1
7GGLY1000.4554257765.5924.435T0
8FPHE14.4-2.3376009536.2955.595T0
9NASN98.40.4032196696.4054.493T0
10EGLU1000.4554257764.4394.246T0
11KLYS1000.4554257764.0323.886C0
12TTHR1000.4554257764.6172.515C1
13HHIS1000.4554257765.7352.196C3
14VVAL99.60.4423742495.4312.702E2
15QGLN99.60.4423742495.2183.015E2
16LLEU1000.4554257764.2782.559E1
17SSER1000.4554257764.1093.006E2
18LLEU1000.4554257763.1342.038E2
19PPRO1000.4554257763.0222.427E5
20VVAL1000.4554257762.8352.515E7
21LLEU1000.4554257762.9482.216E5
22QGLN1000.4554257763.3352.764T1
23VVAL1000.4554257762.822.218T0
24RARG93.60.2466013473.6823.146T1
25DASP1000.4554257763.5172.509T2
26VVAL1000.4554257762.7611.733C1
27LLEU1000.4554257763.0262.937C0
28VVAL1000.4554257762.5952.161C0
29RARG1000.4554257762.2121.858C0
30GGLY1000.4554257763.0931.746C2
31FPHE1000.4554257763.42.063C8
32GGLY1000.4554257764.4022.812C10
33DASP1000.4554257764.9393.466C8
34SSER93.60.2466013475.5714.101H10
35VVAL1000.4554257764.9433.712H10
36EGLU1000.4554257765.4754.503H7
37EGLU1000.4554257766.0934.342H10
38VVAL12.4-2.4028585864.7692.995H10
39LLEU99.20.4293227224.5312.781H10
40SSER91.60.1813437144.9093.576H8
41EGLU1000.4554257763.8573.269H9
42AALA99.60.4423742492.942.275H5
43RARG1000.4554257763.5532.89H5
44QGLN14.4-2.3376009533.6453.822H6
45HHIS1000.4554257762.8232.747H6
46LLEU1000.4554257762.842.622H7
47KLYS99.20.4293227223.8393.796H6
48DASP14-2.3506524793.3933.649H6
49GGLY1000.4554257763.1713.18H6
50TTHR1000.4554257762.6662.978E9
51CCYS1000.4554257762.122.136E7
52GGLY1000.4554257761.8161.495E0
53LLEU960.3249105081.6331.807E0
54VVAL99.60.4423742491.9041.854E0
55EGLU99.20.4293227223.2792.872C0
56VVAL13.6-2.3637040064.0642.727C1
57EGLU99.20.4293227224.4493.613C2
58KLYS1000.4554257765.2434.453C2
59GGLY1000.4554257765.8553.443C3
60VVAL99.60.4423742494.5332.936G2
61LLEU1000.4554257762.9212.754G3
62PPRO94.40.2727044015.282.942G3
63QGLN1000.4554257764.8052.868G2
64LLEU99.20.4293227223.1851.884C2
65EGLU1000.4554257764.0292.115C2
66QGLN94.40.2727044014.2043.445C4
67PPRO1000.4554257763.7232.711C10
68YTYR1000.4554257763.4822.216E7
69VVAL99.60.4423742492.8631.793E8
70FPHE1000.4554257762.5861.86E3
71IILE99.20.4293227222.7361.583E2
72KLYS1000.4554257763.4852.408E2
73RARG1000.4554257763.6342.224C1
74SSER99.60.4423742495.231.987C0
75DASP93.60.2466013476.2823.04C0
76AALA1000.4554257768.7043.228C0
77RARG14-2.3506524798.3773.022C0
78TTHR14.4-2.3376009537.1883.598C0
79AALA14.4-2.3376009537.0812.87C0
80PPRO14-2.3506524796.4443.444C0
81HHIS1000.4554257767.8354.051C1
82GGLY98.40.4032196697.8543.077C1
83HHIS98.80.4162711955.5962.63C0
84VVAL14-2.3506524794.1752.08E0
85MMET14-2.3506524794.4142.089E0
86VVAL95.20.2988074544.0231.397E0
87EGLU1000.4554257764.2241.768E1
88LLEU1000.4554257763.7761.936E2
89VVAL1000.4554257764.2652.449E5
90AALA1000.4554257764.5632.737E8
91EGLU1000.4554257764.8783.084E7
92LLEU39.6-1.5153547665.6033.709C9
93EGLU13.2-2.3767555335.8163.963C6
94GGLY1000.4554257764.2253.123C5
95IILE940.2596528745.0464.119C5
96QGLN99.60.4423742494.6263.922C6
97YTYR86.40.0116738663.7772.983T7
98GGLY1000.4554257763.242.773T7
99RARG1000.4554257764.3684.195T6
100SSER1000.4554257764.2543.136T6
101GGLY1000.4554257763.6293.029C4
102EGLU14.4-2.3376009533.632.66C4
103TTHR99.60.4423742492.5942.144C5
104LLEU1000.4554257762.0821.976E1
105GGLY1000.4554257761.8521.475E1
106VVAL1000.4554257761.4941.516E0
107LLEU1000.4554257761.5381.523E0
108VVAL1000.4554257761.6781.32E0
109PPRO1000.4554257762.0481.906E2
110HHIS99.20.4293227222.2612.571C4
111VVAL99.20.4293227223.2473.537C5
112GGLY99.60.4423742493.6743.367C3
113EGLU1000.4554257763.6412.723C2
114IILE12.4-2.4028585864.5893.838C3
115PPRO1000.4554257764.1533.247C7
116VVAL14.4-2.3376009534.9893.574E6
117AALA98.40.4032196694.7563.548E6
118YTYR1000.4554257764.3273.625E7
119RARG1000.4554257764.2942.977E5
120KLYS14.4-2.3376009534.733.55E4
121VVAL1000.4554257764.7662.026E1
122LLEU1000.4554257765.432.048E0
123LLEU99.20.4293227225.4611.92E2
124RARG1000.4554257765.872.4C2
125KLYS1000.4554257766.233.411C2
126NASN99.20.4293227226.5283.371C1
127GGLY1000.4554257764.8592.463C0
128NASN1000.4554257762.6292.9C0
129KLYS1000.4554257764.0182.985C0
130GGLY98.80.4162711955.1422.464C0
131AALA1000.4554257764.7863.334C0
132GGLY1000.4554257764.5653.283C0
133GGLY1000.4554257767.0663.882T0
134HHIS1000.45542577610.1585.474T0
135SSER99.20.4293227228.4914.808T0
136YTYR76.8-0.3015627779.5784.689T0
137GGLY1000.45542577611.5494.329C0
138AALA14.4-2.33760095311.6334.931C0
139DASP1000.45542577611.7074.98C1
140LLEU1000.45542577613.25.894C2
141KLYS96.80.35101356212.365.953C2
142SSER99.20.42932272213.2444.392C2
143FPHE13.2-2.37675553310.0383.528C2
144DASP1000.4554257769.6113.808C2
145LLEU1000.4554257768.2944.457C2
146GGLY1000.4554257766.4794.715T3
147DASP98.80.4162711956.735.713T4
148EGLU96.40.3379620359.595.356T3
149LLEU1000.4554257765.2913.677T6
150GGLY94.80.2857559288.2994.462C4
151TTHR1000.45542577610.7674.742C4
152DASP1000.4554257767.0663.879C4
153PPRO99.20.4293227224.5753.915C2
154YTYR12.8-2.389807065.2644.089C3
155EGLU99.60.4423742493.7713.24H3
156DASP1000.4554257763.8183.876H1
157FPHE14-2.3506524794.0624.108H2
158QGLN14-2.3506524793.4552.955H0
159EGLU13.6-2.3637040063.9433.091H1
160NASN93.20.2335498214.0343.364H1
161WTRP1000.4554257765.6544.048H1
162NASN1000.4554257766.5533.448C2
163TTHR1000.4554257766.7264.14H2
164KLYS1000.4554257767.74.912H2
165HHIS1000.4554257766.6454.162H2
166SSER12.8-2.389807065.9283.871H3
167SSER95.20.2988074546.2444.62H3
168GGLY1000.4554257768.1194.558H2
169VVAL29.2-1.8546944628.5244.344H4
170TTHR13.6-2.3637040067.3374.557H5
171RARG1000.4554257768.576.01H7
172EGLU98.80.41627119510.975.582H6
173LLEU1000.45542577611.0135.524H8
174MMET12.8-2.3898070610.1215.981H7
175RARG1000.45542577612.3426.805H7
176EGLU1000.45542577614.5286.742H6
177LLEU1000.45542577614.1156.876H5
178NASN1000.45542577615.8667.296C6
179GGLY1000.45542577620.4368.047C5
180GGLY1000.45542577625.0939.249C1

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

3.            Cui H, Gao Z, Liu M, et al. Structural Genomics and Interactomics of 2019 Wuhan Novel Coronavirus, SARS-CoV-2, Indicate Evolutionary Conserved Functional Regions of Viral Proteins. 2020 [cited 2020 Apr 10];Available from: https://www.preprints.org/manuscript/202002.0372/v1