Membrane (M) Google Drive Data

Membrane (M)– the SARS-CoV M protein is predicted to be a triple-spanning membrane protein. The first trans-membrane region (a.a. 14–36) is predicted to be stabilized by the interaction with other trans-membrane segments. The second and third trans-membrane regions (a.a. 46–68 and 78–100) likely do not require stabilization to penetrate the membrane.³⁷

Membrane (M) 1,507 Sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
1	M	MET	1.128069011	-0.905366141	18.644	11.593	C	1
2	A	ALA	0.464499005	-0.925965842	15.72	9.927	C	2
3	D	ASP	0.530856005	-0.923905872	13.703	10.084	C	2
4	S	SER	2.786994028	-0.853866888	13.039	9.072	C	2
5	N	ASN	20.3052422	-0.31003478	9.287	7.656	C	1
6	G	GLY	3.715992037	-0.825027307	10.194	6.003	C	1
7	T	THR	8.294625083	-0.682889369	6.883	4.715	C	1
8	I	ILE	4.97677505	-0.785887875	6.549	3.171	C	0
9	T	THR	13.60318514	-0.518091761	6.301	3.455	C	2
10	V	VAL	4.246848042	-0.808547546	4.155	3.185	C	3
11	E	GLU	4.578633046	-0.798247695	4.891	3.21	H	3
12	E	GLU	12.01061712	-0.567531043	5.256	2.892	H	4
13	L	LEU	17.78367618	-0.388313644	4.484	2.152	H	2
14	K	LYS	2.98606503	-0.847686978	3.92	2.557	H	2
15	K	LYS	6.502986065	-0.738508562	3.408	2.704	H	2
16	L	LEU	31.38686131	0.033980228	2.46	1.965	H	0
17	L	LEU	50.1658925	0.616951768	1.686	1.488	H	0
18	E	GLU	4.578633046	-0.798247695	2.13	1.573	H	0
19	Q	GLN	3.118779031	-0.843567038	2.124	1.876	H	0
20	W	TRP	57.66423358	0.84972839	1.342	1.46	C	0
21	N	ASN	82.68082283	1.62633712	1.751	1.438	C	0
22	L	LEU	31.91771732	0.050459988	2.181	1.58	C	0
23	V	VAL	2.919708029	-0.849746948	2.939	1.699	H	0
24	I	ILE	29.46250829	-0.025758905	3.205	1.661	H	0
25	G	GLY	11.41340411	-0.586070774	2.645	1.062	H	0
26	F	PHE	2.98606503	-0.847686978	2.781	1.347	H	0
27	L	LEU	3.317850033	-0.837387127	3.739	1.669	H	0
28	F	PHE	7.830126078	-0.69730916	4.059	1.998	H	0
29	L	LEU	44.79097545	0.450094189	3.233	1.843	H	0
30	T	THR	1.857996019	-0.88270647	2.436	1.456	H	0
31	W	TRP	2.919708029	-0.849746948	4.481	1.862	H	0
32	I	ILE	23.42402123	-0.213216185	4.455	1.732	H	0
33	C	CYS	0.464499005	-0.925965842	3.407	1.786	H	0
34	L	LEU	29.7279363	-0.017519025	5.052	1.551	H	0
35	L	LEU	87.52488388	1.776714937	6.153	1.99	H	0
36	Q	GLN	89.25016589	1.83027416	4.795	2.467	H	0
37	F	PHE	12.14333112	-0.563411103	4.941	2.632	H	1
38	A	ALA	3.118779031	-0.843567038	5.057	2.242	C	2
39	Y	TYR	47.71068348	0.540732874	4.286	3.312	C	1
40	A	ALA	31.38686131	0.033980228	3.404	2.397	C	0
41	N	ASN	2.98606503	-0.847686978	5.001	2.154	C	0
42	R	ARG	47.64432648	0.538672904	4.491	1.769	H	0
43	N	ASN	6.901128069	-0.726148742	5.778	2.142	H	0
44	R	ARG	33.90842734	0.112259092	6.902	2.541	H	0
45	F	PHE	48.24153948	0.557212635	5.322	2.079	H	0
46	L	LEU	27.73722628	-0.079318128	4.307	1.798	H	0
47	Y	TYR	98.20836098	2.108370124	5.357	3.015	H	1
48	I	ILE	31.91771732	0.050459988	4.621	1.671	H	1
49	I	ILE	38.22163238	0.246157149	4.037	1.844	H	1
50	K	LYS	99.40278699	2.145449586	4.115	3.038	H	1
51	L	LEU	5.773059058	-0.761168233	3.088	1.858	H	0
52	I	ILE	36.29727936	0.186418015	2.944	1.473	H	0
53	F	PHE	3.185136032	-0.841507067	4.427	2.163	H	0
54	L	LEU	71.86463172	1.290561992	4.285	2.175	H	1
55	W	TRP	99.40278699	2.145449586	3.367	1.521	H	2
56	L	LEU	52.02388852	0.674630931	4.27	2.326	H	1
57	L	LEU	62.24286662	0.991866327	1.803	2.1	H	0
58	W	TRP	96.35036496	2.050690961	3.412	2.105	T	0
59	P	PRO	99.668215	2.153689467	5.799	2.244	T	1
60	V	VAL	4.313205043	-0.806487576	7.798	2.424	T	1
61	T	THR	13.93497014	-0.50779191	6.857	3.037	T	0
62	L	LEU	55.60716656	0.785869316	5.578	3.224	C	0
63	A	ALA	88.38752488	1.803494549	4.58	1.783	C	0
64	C	CYS	3.052422031	-0.845627008	2.433	1.653	H	0
65	F	PHE	3.185136032	-0.841507067	4.732	2.571	H	0
66	V	VAL	32.91307233	0.08135954	6.35	2.109	H	0
67	L	LEU	3.052422031	-0.845627008	4.447	1.685	H	0
68	A	ALA	5.04313205	-0.783827905	1.666	1.685	H	0
69	A	ALA	31.18779031	0.027800317	4.498	2.3	H	0
70	V	VAL	13.86861314	-0.50985188	6.204	3.837	C	1
71	Y	TYR	40.74319841	0.324436013	5.777	3.689	C	1
72	R	ARG	3.317850033	-0.837387127	5.901	4.584	C	1
73	I	ILE	8.493696085	-0.676709459	5.275	3.666	T	1
74	N	ASN	36.03185136	0.178178135	4.37	3.732	T	1
75	W	TRP	27.60451228	-0.083438068	3.672	2.678	T	2
76	I	ILE	0.729927007	-0.917725961	3.309	2.451	T	1
77	T	THR	3.516921035	-0.831207217	2.758	2.247	C	0
78	G	GLY	2.919708029	-0.849746948	1.71	1.828	C	0
79	G	GLY	39.6814864	0.291476491	1.631	1.751	C	0
80	I	ILE	6.96748507	-0.724088771	1.61	2.464	T	0
81	A	ALA	4.246848042	-0.808547546	1.275	1.571	T	0
82	I	ILE	79.49568679	1.527458555	1.404	1.51	T	0
83	A	ALA	30.3251493	0.001020706	1.714	1.597	T	0
84	M	MET	27.47179827	-0.087558009	1.471	1.907	C	0
85	A	ALA	59.45587259	0.905347582	1.37	1.579	C	0
86	C	CYS	26.14465826	-0.128757411	1.177	1.412	T	0
87	L	LEU	3.384207034	-0.835327157	1.178	1.608	T	0
88	V	VAL	3.649635036	-0.827087277	1.19	1.512	T	0
89	G	GLY	4.180491042	-0.810607516	2.013	0.887	T	0
90	L	LEU	23.02587923	-0.225576006	0.923	1.514	T	0
91	M	MET	17.31917717	-0.402733435	1.366	1.441	T	0
92	W	TRP	70.60384871	1.25142256	2.716	1.643	T	0
93	L	LEU	9.489051095	-0.645809907	4.853	1.761	T	0
94	S	SER	7.232913072	-0.715848891	4.736	1.915	C	0
95	Y	TYR	99.734572	2.155749437	4.132	3.078	C	0
96	F	PHE	67.28599867	1.148424055	5.091	2.435	C	0
97	I	ILE	31.85136032	0.048400018	5.95	2.664	C	0
98	A	ALA	3.052422031	-0.845627008	5.324	2.59	C	0
99	S	SER	98.80557399	2.126909855	9.321	3.366	T	1
100	F	PHE	16.58925017	-0.425393106	9.487	4.983	T	2
101	R	ARG	71.33377571	1.274082231	8.809	5.973	T	2
102	L	LEU	99.601858	2.151629497	6.996	4.151	T	1
103	F	PHE	45.05640345	0.458334069	7.38	3.872	T	0
104	A	ALA	2.98606503	-0.847686978	6.097	3.339	T	0
105	R	ARG	97.14664897	2.075410603	5.042	3.983	T	0
106	T	THR	68.81220969	1.195803367	4.871	3.326	T	1
107	R	ARG	37.02720637	0.209077687	5.775	4.885	C	3
108	S	SER	94.16058394	1.982711948	6.154	4.702	C	2
109	M	MET	2.786994028	-0.853866888	4.999	3.565	C	3
110	W	TRP	99.601858	2.151629497	8.01	4.607	C	2
111	S	SER	87.72395488	1.782894848	7.143	3.8	C	1
112	F	PHE	95.02322495	2.009491559	6.84	4.263	C	0
113	N	ASN	94.75779695	2.001251679	6.757	4.456	C	1
114	P	PRO	99.46914399	2.147509556	8.57	4.316	C	2
115	E	GLU	98.67285999	2.122789915	8.062	4.536	C	2
116	T	THR	66.82149967	1.134004264	7.659	4.082	C	1
117	N	ASN	72.66091573	1.315281634	8.391	3.93	C	1
118	I	ILE	2.98606503	-0.847686978	7.17	3.095	C	1
119	L	LEU	36.03185136	0.178178135	4.639	2.91	C	0
120	L	LEU	53.94824154	0.734370064	4.419	3.58	C	0
121	N	ASN	8.161911082	-0.68700931	3.362	2.317	C	0
122	V	VAL	36.09820836	0.180238105	2.23	3.111	C	0
123	P	PRO	8.095554081	-0.68906928	2.851	2.863	C	0
124	L	LEU	4.313205043	-0.806487576	3.078	1.846	C	0
125	H	HIS	0.265428003	-0.932145752	3.572	1.436	C	0
126	G	GLY	99.535501	2.149569527	4.257	1.919	C	0
127	T	THR	11.47976111	-0.584010804	3.437	2.103	C	0
128	I	ILE	3.052422031	-0.845627008	2.165	1.817	C	0
129	L	LEU	0.928998009	-0.911546051	2.421	2.274	C	0
130	T	THR	4.97677505	-0.785887875	2.524	2.033	C	0
131	R	ARG	16.45653616	-0.429513046	1.583	1.882	C	0
132	P	PRO	70.73656271	1.255542501	1.51	1.468	C	0
133	L	LEU	34.63835435	0.134918763	1.204	1.706	T	0
134	L	LEU	21.69873922	-0.266775408	2.303	1.77	T	1
135	E	GLU	15.46118115	-0.460412598	2.773	2.197	T	1
136	S	SER	3.052422031	-0.845627008	1.766	1.63	T	0
137	E	GLU	2.98606503	-0.847686978	1.658	1.689	C	1
138	L	LEU	3.052422031	-0.845627008	1.326	1.655	C	1
139	V	VAL	31.85136032	0.048400018	1.39	1.201	C	0
140	I	ILE	9.820836098	-0.635510057	2.448	1.882	C	0
141	G	GLY	2.919708029	-0.849746948	2.493	1.238	C	0
142	A	ALA	10.88254811	-0.602550535	2.426	1.55	E	0
143	V	VAL	9.555408096	-0.643749937	2.12	1.675	E	0
144	I	ILE	40.2786994	0.310016222	1.888	1.651	C	0
145	L	LEU	52.68745853	0.695230632	1.473	1.456	C	0
146	R	ARG	12.67418713	-0.546931342	2.447	1.934	C	0
147	G	GLY	99.07100199	2.135149736	5.662	1.738	C	0
148	H	HIS	13.86861314	-0.50985188	7.941	3.315	C	1
149	L	LEU	98.14200398	2.106310154	6.391	2.514	C	1
150	R	ARG	3.317850033	-0.837387127	5.141	2.505	C	1
151	I	ILE	4.844061048	-0.790007815	4.662	2.737	C	1
152	A	ALA	6.96748507	-0.724088771	4.461	3.016	C	0
153	G	GLY	99.27007299	2.141329646	5.408	2.496	C	0
154	H	HIS	5.242203052	-0.777647994	4.733	2.542	C	1
155	H	HIS	4.180491042	-0.810607516	5.399	3.133	C	2
156	L	LEU	44.72461845	0.448034219	6.226	3.092	C	1
157	G	GLY	17.45189117	-0.398613495	3.174	2.059	C	0
158	R	ARG	4.445919044	-0.802367635	4.303	2.763	C	0
159	C	CYS	36.42999336	0.190537956	5.855	2.036	C	0
160	D	ASP	8.161911082	-0.68700931	3.893	2.575	C	0
161	I	ILE	2.98606503	-0.847686978	4.002	2.215	T	1
162	K	LYS	2.919708029	-0.849746948	3.876	3.567	T	1
163	D	ASP	12.34240212	-0.557231193	3.994	1.917	T	0
164	L	LEU	95.15593895	2.0136115	4.282	1.764	T	0
165	P	PRO	99.20371599	2.139269676	5.483	1.932	C	0
166	K	LYS	58.32780358	0.870328091	4.398	2.447	C	0
167	E	GLU	7.03384207	-0.722028801	2.714	2.26	C	0
168	I	ILE	33.51028534	0.099899271	2.285	2.057	C	0
169	T	THR	39.41605839	0.28323661	2.906	2.759	C	0
170	V	VAL	78.50033179	1.496559003	2.692	2.549	C	0
171	A	ALA	68.34771068	1.181383577	2.363	2.293	C	0
172	T	THR	52.68745853	0.695230632	3.545	2.55	T	0
173	S	SER	3.384207034	-0.835327157	3.969	1.835	T	0
174	R	ARG	3.118779031	-0.843567038	5.077	3.164	T	0
175	T	THR	27.67086928	-0.081378098	4.757	1.68	T	0
176	L	LEU	16.05839416	-0.441872867	3.434	2.389	E	0
177	S	SER	3.118779031	-0.843567038	4.49	2.808	E	0
178	Y	TYR	3.052422031	-0.845627008	4.314	2.666	E	0
179	Y	TYR	94.35965494	1.988891858	3.748	1.352	E	0
180	K	LYS	16.78832117	-0.419213196	3.818	2.366	E	0
181	L	LEU	28.59986729	-0.052538517	2.568	2.128	E	0
182	G	GLY	7.96284008	-0.69318922	3.597	1.593	E	0
183	A	ALA	3.251493033	-0.839447097	2.766	2.237	C	0
184	S	SER	3.185136032	-0.841507067	3.562	1.852	C	0
185	Q	GLN	27.47179827	-0.087558009	2.741	2.403	C	0
186	R	ARG	5.573988056	-0.767348144	2.517	2.639	C	0
187	V	VAL	5.04313205	-0.783827905	2.189	1.673	C	0
188	A	ALA	47.17982747	0.524253113	3.4	1.874	C	0
189	G	GLY	2.256138023	-0.870346649	3.129	1.834	C	1
190	D	ASP	3.384207034	-0.835327157	3.604	1.542	C	1
191	S	SER	22.16323822	-0.252355617	2.108	1.376	C	0
192	G	GLY	15.39482415	-0.462472568	1.503	1.144	C	0
193	F	PHE	41.40676841	0.345035714	2.086	1.282	C	0
194	A	ALA	93.76244194	1.970352127	2.1	1.205	E	0
195	A	ALA	2.786994028	-0.853866888	2.278	1.204	E	0
196	Y	TYR	65.16257465	1.082505012	2.235	1.4	E	0
197	S	SER	0.331785003	-0.930085782	2.961	1.474	E	0
198	R	ARG	30.3251493	0.001020706	3.618	1.343	E	0
199	Y	TYR	7.896483079	-0.69524919	5.042	2.019	E	0
200	R	ARG	3.052422031	-0.845627008	7.017	4.145	E	1
201	I	ILE	2.919708029	-0.849746948	7.753	3.928	E	2
202	G	GLY	66.62242867	1.127824354	7.887	3.995	C	2
203	N	ASN	16.39017916	-0.431573016	9.463	5.22	C	2
204	Y	TYR	62.70736563	1.006286118	8.316	5.343	C	2
205	K	LYS	3.118779031	-0.843567038	10.801	6.11	C	3
206	L	LEU	11.08161911	-0.596370625	8.632	4.977	C	2
207	N	ASN	4.313205043	-0.806487576	9.879	5.552	C	2
208	T	THR	9.289980093	-0.651989818	7.174	4.033	C	1
209	D	ASP	3.516921035	-0.831207217	8.409	4.653	C	2
210	H	HIS	3.052422031	-0.845627008	8.8	4.608	T	2
211	S	SER	13.93497014	-0.50779191	7.368	4.205	T	2
212	S	SER	9.090909091	-0.658169728	7.918	4.37	T	2
213	S	SER	2.919708029	-0.849746948	6.959	4.02	T	2
214	S	SER	30.2587923	-0.001039264	9.905	4.721	C	2
215	D	ASP	22.56138023	-0.239995797	6.972	3.803	C	1
216	N	ASN	5.573988056	-0.767348144	2.834	2.826	C	0
217	I	ILE	3.052422031	-0.845627008	4.3	3.602	C	0
218	A	ALA	18.24817518	-0.373893853	4.626	3.71	C	0
219	L	LEU	45.12276045	0.46039404	5.85	3.856	C	0
220	L	LEU	94.16058394	1.982711948	7.917	3.4	C	0
221	V	VAL	3.715992037	-0.825027307	7.172	3.732	C	0
222	Q	GLN	3.118779031	-0.843567038	4.408	2.755	C	0

37.         Ma H-C, Fang C-P, Hsieh Y-C, Chen S-C, Li H-C, Lo S-Y. Expression and membrane integration of SARS-CoV M protein. J Biomed Sci 2008;15(3):301–10.