Helicase Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

Helicase (Hel) (nsp13)– it has a zinc-binding domain in its N-terminus and it displays RNA and DNA duplex-unwinding activities with 5′ to 3′ polarity.2 It also possesses the nucleoside triphosphate hydrolase (NTPase) that can hydrolyze all types of NTPs. Its helicase-activity is dependent on magnesium and NTPs.2,23 Nsp13 is predicted to contain 596 amino acids. It has a triangular pyramid shape comprising five domains. Domains 1A (261-441 a.a), 2A (442-596 a.a), and 1B (150-260 a.a) form the triangular base. N-terminal zinc binding domain (ZBD) (1-99 a.a) and stalk domain (100-149 a.a) are arranged at the apex of the pyramid. NTPase active site residues include Lys288, Ser289, Asp374, Glu375, Gln404 and Arg567.24 Bismuth salts could effectively inhibit both the NTPase and RNA helicase activities of nsp13 in a dose-dependent manner.23

Helicase 5,598 Sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
5325AALA19.20328689-0.7613479993.7312.337C0
5326VVAL19.04251518-0.7706003763.0192.075C0
5327GGLY45.212575920.7354810282.4391.249E1
5328AALA10.05716327-1.2877054592.3861.272E2
5329CCYS45.141121830.731368861.8970.942C1
5330VVAL44.498035010.6943593511.6341.442C0
5331LLEU4.448017149-1.610510623.2631.742C0
5332CCYS45.176848870.7334249443.8571.26C1
5333NASN13.82636656-1.0707886153.7582.052C2
5334SSER45.516255810.752957743.7011.361E2
5335QGLN43.033226150.6100599143.4971.96E2
5336TTHR45.284030010.7395931952.8781.138E1
5337SSER18.91747053-0.777796672.7131.318C0
5338LLEU45.74848160.7663222851.7931.078C0
5339RARG45.873526260.7735185781.9411.357E0
5340CCYS45.141121830.731368861.4770.729E0
5341GGLY46.320114330.7992196261.6840.991C0
5342AALA4.573061808-1.6033143261.3461.061C0
5343CCYS45.158985350.7323969021.1240.79T0
5344IILE29.72490175-0.15583131211.164T0
5345RARG45.284030010.7395931950.8730.885T0
5346RARG30.26080743-0.1249900541.0471.512T0
5347PPRO47.034655230.8403413031.3860.809C0
5348FPHE20.22150768-0.702749611.9151.035C0
5349LLEU46.802429440.8269767581.9721.002C1
5350CCYS45.176848870.7334249442.0420.826H1
5351CCYS28.42086459-0.2308783712.5271.03H0
5352KLYS43.604858880.6429572552.2911.72H1
5353CCYS45.212575920.7354810282.2110.744H1
5354CCYS25.72347267-0.38611272.0350.736H0
5355YTYR45.069667740.7272566922.0921.036H0
5356DASP43.354769560.6285646692.2510.993H0
5357HHIS45.19471240.7344529861.7850.74H0
5358VVAL46.92747410.8341730511.5461.014H1
5359IILE22.38299393-0.5783565382.0171.269H1
5360SSER6.627366917-1.4850895062.4031.067C1
5361TTHR46.480886030.8084720031.9261.083C2
5362SSER7.359771347-1.4429397881.7411.18C2
5363HHIS47.177563420.8485656381.3550.891C1
5364KLYS44.676670240.704639771.3761.249C0
5365LLEU7.056091461-1.46041651.4641.131E0
5366VVAL30.45730618-0.1136815931.6110.992E0
5367LLEU48.231511250.9092201111.711.199E0
5368SSER29.27831368-0.181532362.2561.419C0
5369VVAL19.34619507-0.7531236642.8582.137C0
5370NASN13.32618792-1.0995737892.5371.801C1
5371PPRO83.369060382.9313785552.2721.455C2
5372YTYR45.033940690.7252006081.9411.411C2
5373VVAL35.387638440.1700579752.4141.529C3
5374CCYS45.069667740.7272566922.7811.603C6
5375NASN21.77563416-0.6133099633.571.975C5
5376AALA29.74276527-0.154803274.0642.124T5
5377PPRO25.93783494-0.3737761976.2582.505T5
5378GGLY31.79707038-0.036578455.8822.439T2
5379CCYS45.212575920.7354810283.9462.049T6
5380DASP11.68274384-1.1941536453.1722.197C1
5381VVAL37.888531620.3139838432.2991.978C1
5382TTHR4.501607717-1.6074264942.2511.874C0
5383DASP42.801000360.5966953691.9671.916T0
5384VVAL53.269024651.1991279311.6211.517T0
5385TTHR43.283315470.6244525011.6731.741T0
5386QGLN7.734905323-1.4213509083.1921.939T0
5387LLEU79.5284032.7103495442.1681.684C2
5388YTYR41.300464450.5103398482.2931.673E5
5389LLEU44.480171490.6933313092.8492.131E3
5390GGLY43.015362630.6090318723.2662.106C1
5391GGLY45.373347620.7447334054.6992.59C1
5392MMET26.54519471-0.3388227725.1683.274C2
5393SSER39.460521610.4044515313.4452.088E2
5394YTYR42.747409790.5936112432.2421.54E3
5395YTYR18.84601643-0.7819088372.0321.815E6
5396CCYS44.980350130.7221164832.4791.709T5
5397KLYS4.876741693-1.5858376142.9592.585T3
5398SSER3.697749196-1.653688383.2332.261T3
5399HHIS53.876384421.2340813562.6222.109T5
5400KLYS41.371918540.5144520162.3292.188C4
5401PPRO45.033940690.7252006082.8062.33C4
5402PPRO4.448017149-1.610510624.8342.79C3
5403IILE17.84565916-0.8394791844.7522.937C3
5404SSER32.475884240.0024871433.7822.061C6
5405FPHE35.530546620.178282314.1562.727C2
5406PPRO38.210075030.3324885973.5281.661C3
5407LLEU42.926045020.6038916632.5041.548E1
5408CCYS28.51018221-0.2257381623.3721.38E0
5409AALA17.18470882-0.8775167354.0481.567E0
5410NASN37.191854230.2738902083.2131.935T0
5411GGLY45.069667740.7272566922.5291.154T0
5412QGLN4.358699536-1.6156508292.2041.625E0
5413VVAL66.291532691.9485704862.5051.388E0
5414FPHE44.944623080.7200603992.611.487C0
5415GGLY45.409074670.7467894892.8081.344T1
5416LLEU56.823865671.4037082723.7162.231T1
5417YTYR45.123258310.7303408184.0821.774T0
5418KLYS37.441943550.2882827954.4642.611T1
5419NASN25.58056449-0.3943370364.4142.637C1
5420TTHR7.949267596-1.4090144053.3372.032C0
5421CCYS26.81314755-0.3234021443.0571.68C0
5422VVAL9.199714184-1.3370514712.8942.058C0
5423GGLY45.35548410.7437053632.5511.194C0
5424SSER46.838156480.8290328422.671.193C0
5425DASP6.859592712-1.4717249613.671.606C0
5426NASN10.82529475-1.2434996572.5841.317H0
5427VVAL28.42086459-0.2308783712.4981.496H0
5428TTHR4.465880672-1.6094825782.2331.47H0
5429DASP31.90425152-0.0304101982.2891.031H1
5430FPHE45.141121830.731368862.2380.839H1
5431NASN45.016077170.7241725662.1860.907H0
5432AALA3.804930332-1.6475201292.70.993H0
5433IILE20.20364416-0.7037776521.9960.948H0
5434AALA43.604858880.6429572551.9250.92H0
5435TTHR35.655591280.1854786042.6331.024C0
5436CCYS18.81028939-0.7839649212.4921.038C0
5437DASP28.76027153-0.2113455753.6961.114C0
5438WTRP42.890317970.6018355791.6580.895C0
5439TTHR32.27938549-0.0088213181.8171.211E1
5440NASN6.555912826-1.4892016741.8731.291E3
5441AALA4.108610218-1.6300434162.041.096E1
5442GGLY11.66488031-1.1951816872.0850.983H0
5443DASP35.35191140.1680018912.3841.041H0
5444YTYR44.980350130.7221164831.9230.884H1
5445IILE16.41657735-0.9217225381.91.189H2
5446LLEU42.908181490.6028636211.7881.172H1
5447AALA45.998570920.7807148721.4570.923H0
5448NASN45.19471240.7344529861.2460.919C0
5449TTHR13.23687031-1.1047139981.2781.069C0
5450CCYS18.97106109-0.7747125441.3370.888C1
5451TTHR18.57806359-0.7973294661.4851.203H1
5452EGLU23.25830654-0.5279824851.5981.144H0
5453RARG11.16470168-1.223966861.3191.836H0
5454LLEU45.051804220.726228651.3820.949H0
5455KLYS37.495534120.2913669211.3841.094H0
5456LLEU37.138263670.2708060831.0020.886H1
5457FPHE44.765987850.709779981.0670.774H1
5458AALA47.409789210.8619301831.2050.822H0
5459AALA46.677384780.8197804641.1640.848H1
5460EGLU45.641300460.7601540330.8990.828H2
5461TTHR45.105394780.7293127761.0380.805H2
5462LLEU12.93319043-1.1221907111.3061.149H2
5463KLYS36.69167560.2451050351.1951.082H2
5464AALA45.016077170.7241725660.8640.903H2
5465TTHR26.27724187-0.3542434011.040.894H2
5466EGLU45.051804220.726228651.1071.132H2
5467EGLU45.087531260.7282847341.0320.872H2
5468TTHR3.769203287-1.6495762131.1330.979H2
5469FPHE10.25366202-1.2763969981.3221.108H2
5470KLYS44.96248660.7210884411.2641.054H3
5471LLEU3.876384423-1.6434079611.3141.322C2
5472SSER36.495176850.2337965741.3991.248C3
5473YTYR38.263665590.3355727231.5331.352C3
5474GGLY7.734905323-1.4213509081.7191.402C2
5475IILE11.82565202-1.1859293091.6831.707E7
5476AALA50.303679891.0284729731.5181.604E10
5477TTHR36.084315830.210151611.6521.792E14
5478VVAL13.95141122-1.0635923221.8111.785E15
5479RARG14.34440872-1.04097541.8982.867E11
5480EGLU48.713826370.9369772431.8992.373E11
5481VVAL29.40335834-0.1743360662.0671.936C8
5482LLEU9.914255091-1.2959297941.9692.177C5
5483SSER20.471597-0.6883570231.7561.761C3
5484DASP15.46981065-0.9762087591.8632.006C1
5485RARG29.09967846-0.1918127791.4082.682E0
5486EGLU37.013219010.2636097891.3861.722E1
5487LLEU42.479456950.5781906151.3281.839E5
5488HHIS3.751339764-1.6506042541.6052.123E8
5489LLEU49.535548410.9842671711.6571.709E8
5490SSER22.41872097-0.5763004541.6731.958E9
5491WTRP45.58770990.7570699081.9391.782E9
5492EGLU45.176848870.7334249441.8961.841C6
5493VVAL20.90032154-0.6636840172.1282.307C2
5494GGLY47.838513750.8866031892.2232.218C1
5495KLYS35.90568060.199871192.0462.158C1
5496PPRO15.29117542-0.9864891781.9751.634C2
5497RARG14.82672383-1.0132182682.111.928C2
5498PPRO45.98070740.779686832.4311.368C1
5499PPRO45.033940690.7252006082.421.458C0
5500LLEU43.962129330.6635180941.6961.77C0
5501NASN42.67595570.5894990762.4821.653T0
5502RARG36.29867810.2224881131.6461.668T0
5503NASN45.802072170.7694064111.4151.726T0
5504YTYR26.20578778-0.3583555691.8261.235T0
5505VVAL42.818863880.5977234111.2471.411E0
5506FPHE47.159699890.8475375960.981.157E1
5507TTHR44.783851380.7108080221.2871.289E4
5508GGLY28.40300107-0.2319064131.3131.254E7
5509YTYR38.317256160.3386568491.6691.588E9
5510RARG3.697749196-1.653688382.122.162E6
5511VVAL4.78742408-1.5909778232.2472.102E4
5512TTHR38.638799570.3571616032.4552.562E2
5513KLYS36.030725260.2070674842.8184.041T1
5514NASN31.11825652-0.0756440424.0573.709T1
5515SSER24.99106824-0.4282624193.4112.635E2
5516KLYS44.640943190.7025836862.3692.793E4
5517VVAL19.73919257-0.7305067422.2762.168E4
5518QGLN38.370846730.3417409751.9911.944E5
5519IILE15.0410861-1.0008817651.7671.566E3
5520GGLY45.087531260.7282847341.5621.223E1
5521EGLU37.048946050.2656658731.2011.236E0
5522YTYR21.34690961-0.6379829691.0411.166E1
5523TTHR12.23651304-1.1622843451.041.233E2
5524FPHE43.033226150.6100599141.2871.503E2
5525EGLU38.067166850.3242642621.8311.928E2
5526KLYS35.316184350.1659458072.4523.222E2
5527GGLY11.64701679-1.1962097291.7281.814C2
5528DASP18.20292962-0.8189183462.3482.24C1
5529YTYR28.63522687-0.2185418682.2513.234T0
5530GGLY28.72454448-0.2134016594.112.528T1
5531DASP38.674526620.3592176872.742.105T2
5532AALA48.517327620.9256687821.7461.798E2
5533VVAL42.801000360.5966953691.3671.576E2
5534VVAL4.769560557-1.5920058651.3891.682E2
5535YTYR44.158628080.6748265551.3261.22E2
5536RARG21.40050018-0.6348988441.191.507E1
5537GGLY7.288317256-1.4470519551.2791.087T0
5538TTHR39.835655590.4260404111.7861.184T0
5539TTHR23.47266881-0.5156459821.8391.716T0
5540TTHR45.551982850.7550138242.1071.998T0
5541YTYR21.93640586-0.6040575861.921.866C1
5542KLYS47.534833870.8691264761.8382.513C2
5543LLEU46.284387280.7971635421.6371.592C4
5544NASN3.644158628-1.6567725062.061.964T5
5545VVAL27.0096463-0.3120936832.12.043T10
5546GGLY50.964630231.0665105242.0471.815T8
5547DASP69.792783142.1500667011.8211.614E11
5548YTYR10.84315827-1.2424716151.5752.09E11
5549FPHE43.015362630.6090318721.3231.413E8
5550VVAL61.146838161.6524944151.2671.673E4
5551LLEU51.96498751.1240808711.5931.489E0
5552TTHR42.658092180.5884710342.761.618C0
5553SSER42.926045020.6038916631.9591.325C0
5554HHIS44.96248660.7210884411.9411.34C0
5555TTHR3.929974991-1.6403238352.2461.345C0
5556VVAL45.016077170.7241725660.8321.05C0
5557MMET3.679885673-1.6547164220.8281.562C0
5558PPRO22.3651304-0.579384580.8070.947C0
5559LLEU45.516255810.752957740.9170.916C0
5560SSER14.43372633-1.035835191.4861.125C0
5561AALA80.903894252.7895087711.2540.948C0
5562PPRO43.837084670.65632180.8750.937C0
5563TTHR40.925330480.4887509681.2170.921C0
5564LLEU24.45516256-0.4591036761.5311.204C0
5565VVAL20.73954984-0.6729363941.751.088C0
5566PPRO18.5066095-0.8014416341.6791.204C0
5567QGLN45.962843870.7786587881.5741.757C0
5568EGLU37.120400140.2697780411.841.368C0
5569HHIS3.965702036-1.6382677511.9651.38C0
5570YTYR37.424080030.2872547532.2391.195C1
5571VVAL14.34440872-1.04097542.6361.778C2
5572RARG13.36191497-1.0975177051.7732.021C2
5573IILE34.405144690.113515671.4061.157C2
5574TTHR14.30868167-1.0430314831.3971.129C1
5575GGLY15.73776349-0.960788131.4111.07C0
5576LLEU37.959985710.3180960111.4091C1
5577YTYR15.00535906-1.0029378491.4361.407C1
5578PPRO38.19221150.3314605551.5561.016C0
5579TTHR13.11182565-1.1119102921.5761.217C0
5580LLEU5.305466238-1.5611646082.1032.128C0
5581NASN20.90032154-0.6636840173.3392.07C0
5582IILE17.43479814-0.8631241482.221.395C0
5583SSER6.573776349-1.4881736321.8751.266T0
5584DASP7.109682029-1.4573323751.6551.477T0
5585EGLU11.59342622-1.1992938541.4791.645T0
5586FPHE26.04501608-0.3676079461.2790.969T0
5587SSER15.6663094-0.9649002981.0521.116C0
5588SSER21.82922472-0.6102258381.1341.164C0
5589NASN18.27438371-0.8148061780.9491.147H0
5590VVAL48.356555910.9164164041.2151.258H0
5591AALA12.14719543-1.1674245551.241.051H0
5592NASN18.07788496-0.826114641.0640.973H0
5593YTYR37.048946050.2656658731.1970.803H1
5594QGLN35.101822080.1536093041.2070.931H2
5595KLYS11.61128975-1.1982658121.0751.283H1
5596VVAL13.07609861-1.1139663751.050.915H0
5597GGLY44.355126830.6861350161.0550.759H0
5598MMET13.09396213-1.1129383331.2191.242H0
5599QGLN30.85030368-0.0910646711.0720.953C0
5600KLYS34.155055380.0991230831.1471.192E0
5601YTYR19.09610575-0.767516250.8981.071E0
5602SSER20.73954984-0.6729363940.6840.717E0
5603TTHR46.177206150.7909952910.8990.853E0
5604LLEU8.288674527-1.3894816081.0321.017E0
5605QGLN45.480528760.7509016560.9380.725E0
5606GGLY48.535191140.9266968241.1230.679E0
5607PPRO75.062522332.4533390661.1360.698T0
5608PPRO47.463379780.8650143091.2430.821T0
5609GGLY53.018935331.1847353440.8540.808T0
5610TTHR24.3301179-0.466299971.1910.889T0
5611GGLY56.663093961.3944558941.4680.948C0
5612KLYS47.71346910.8794068951.4421.063C0
5613SSER45.391211150.7457614471.2080.864C0
5614HHIS44.998213650.7231445251.3251.201H0
5615FPHE21.52554484-0.627702551.0560.972H0
5616AALA29.38549482-0.1753641080.9040.916H0
5617IILE45.712754560.7642662011.0390.99H0
5618GGLY44.837441940.7138921471.1060.744H0
5619LLEU70.685959272.2014687971.0581.085H0
5620AALA29.77849232-0.1527471860.7920.837H0
5621LLEU21.16827438-0.6482633880.8111.21H0
5622YTYR42.943908540.6049197040.8450.79H0
5623YTYR35.673454810.1865066460.8890.712H0
5624PPRO30.92175777-0.0869525041.0670.914C0
5625SSER7.877813505-1.4131265720.9680.992C0
5626AALA45.462665240.7498736140.9230.87C0
5627RARG45.569846370.7560418660.7911.086E0
5628IILE24.74097892-0.4426550060.6260.842E0
5629VVAL42.979635580.6069757880.7170.88E0
5630YTYR22.9903537-0.5434031130.6240.639E0
5631TTHR42.425866380.5751064890.7960.821E0
5632AALA44.694533760.7056678120.690.808E0
5633CCYS35.977134690.2039833580.7460.867C0
5634SSER43.586995360.6419292130.80.852H0
5635HHIS44.765987850.709779980.7020.897H0
5636AALA44.676670240.704639770.8320.96H0
5637AALA44.748124330.7087519380.7870.897H0
5638VVAL43.051089670.6110879560.5930.884H0
5639DASP49.660593070.9914634640.680.762H0
5640AALA33.476241510.060057490.7560.914H0
5641LLEU58.449446231.4972600860.6230.894H0
5642CCYS44.837441940.7138921470.6470.701H1
5643EGLU32.654519470.0127675620.7911.173H1
5644KLYS45.998570920.7807148720.971.36H0
5645AALA45.551982850.7550138240.8540.884H0
5646LLEU4.126473741-1.6290153740.9961.194H0
5647KLYS31.60057163-0.0478869111.4081.704H0
5648YTYR11.86137906-1.1838732261.291.045C0
5649LLEU53.07252591.187819471.1730.996C0
5650PPRO8.092175777-1.4007900691.4621.158T0
5651IILE20.45373348-0.6893850651.6511.73T0
5652DASP28.01000357-0.2545233351.1281.442T0
5653KLYS27.22400857-0.299757181.1261.464E0
5654CCYS46.284387280.7971635420.9010.862E1
5655SSER29.18899607-0.1866725690.8320.842E1
5656RARG74.133619152.3998808860.8531.074E1
5657IILE46.212933190.7930513750.9131.083E1
5658IILE32.940335830.0292162331.1911.338C0
5659PPRO73.079671312.3392264131.5241.279C0
5660AALA37.87066810.3129558011.3731.501C0
5661RARG29.18899607-0.1866725691.5961.895C0
5662AALA32.886745270.0261321071.6851.186C0
5663RARG37.799214010.3088436331.4111.414C0
5664VVAL42.67595570.5894990760.8441.1C0
5665EGLU35.066095030.1515532211.4791.386C0
5666CCYS44.783851380.7108080221.2571.327C0
5667FPHE14.77313326-1.0163023940.941.119C0
5668DASP29.68917471-0.1578873961.4211.361C0
5669KLYS18.43515541-0.8055538010.9681.606C0
5670FPHE44.676670240.704639770.9110.985C1
5671KLYS43.747767060.6511815910.9731.374E2
5672VVAL15.75562701-0.9597600881.1591.551E1
5673NASN46.230796710.7940794171.0651.088E0
5674SSER4.412290104-1.6125667041.2261.029C0
5675TTHR44.105037510.6717424291.721.088C0
5676LLEU4.019292605-1.6351836261.3651.623C1
5677EGLU6.287959986-1.5046223031.1190.963C2
5678QGLN32.08288675-0.0201297790.8430.966E2
5679YTYR45.962843870.7786587880.7130.778E1
5680VVAL16.66666667-0.9073299510.6870.93E0
5681FPHE44.623079670.7015556440.6210.69E0
5682CCYS6.698821008-1.4809773390.7170.826E0
5683TTHR46.355841370.801275710.7650.912T0
5684VVAL21.36477313-0.6369549270.8781.005T0
5685NASN44.80171490.7118360641.0941.282T0
5686AALA45.623436940.7591259921.0441.088T0
5687LLEU45.105394780.7293127761.1171.181C0
5688PPRO47.034655230.8403413031.181.067C0
5689EGLU42.979635580.6069757881.4641.415C0
5690TTHR12.37942122-1.154060011.821.299C0
5691TTHR4.644515899-1.5992021592.071.128C0
5692AALA28.83172562-0.2072334071.0040.919C0
5693DASP46.713111830.8218365480.7640.776E0
5694IILE45.659163990.7611820750.7810.869E0
5695VVAL31.45766345-0.0561112460.8330.901E0
5696VVAL44.533762060.6964154350.7740.873E0
5697FPHE5.448374419-1.5529402730.8760.811E0
5698DASP47.374062170.8598740990.8410.637E0
5699EGLU46.69524830.8208085060.8460.786T0
5700IILE5.894962487-1.5272392250.9491.084T0
5701SSER44.998213650.7231445250.920.755T0
5702MMET44.944623080.7200603990.7061.272T0
5703AALA6.234369418-1.50770642810.928C0
5704TTHR44.980350130.7221164831.2160.93H0
5705NASN45.158985350.7323969020.9950.791H1
5706YTYR44.748124330.7087519380.730.807H2
5707DASP28.77813505-0.2103175330.9260.725H2
5708LLEU45.248302970.7375371110.970.899H2
5709SSER44.873168990.7159482310.9140.686H1
5710VVAL26.42015005-0.3460190661.0560.941H0
5711VVAL7.038227939-1.4614445421.2520.977H0
5712NASN44.765987850.709779981.3230.985H0
5713AALA22.16863165-0.5906930411.2680.972H0
5714RARG38.674526620.3592176871.371.264H0
5715LLEU11.21829225-1.2208827341.7720.969H0
5716RARG11.14683816-1.2249949021.731.827C0
5717AALA18.72097178-0.7891051310.7330.915C0
5718KLYS24.45516256-0.4591036760.8251.75C0
5719HHIS63.70132191.7995044080.8640.74E0
5720YTYR10.66452304-1.2527520340.7930.765E0
5721VVAL45.337620580.7426773210.7680.866E0
5722YTYR44.587352630.6994995610.8810.675E0
5723IILE10.50375134-1.2620044110.9060.854E0
5724GGLY44.891032510.7169762731.110.743E0
5725DASP45.962843870.7786587880.8850.677T0
5726PPRO45.051804220.726228650.8430.846T0
5727AALA26.0628796-0.3665799040.8390.849T0
5728QGLN45.230439440.7365090690.7610.806T0
5729LLEU45.016077170.7241725661.1281.535C0
5730PPRO44.712397280.7066958540.961.067C0
5731AALA45.087531260.7282847340.9511.136C0
5732PPRO44.658806720.7036117281.0751.374C0
5733RARG44.891032510.7169762732.1661.658C0
5734TTHR25.97356199-0.3717201141.3961.603C0
5735LLEU32.26152197-0.009849361.1731.407C1
5736LLEU28.84958914-0.2062053661.6311.269C2
5737TTHR17.5598428-0.8559278551.7071.456C1
5738KLYS16.04144337-0.9433114181.642.003E1
5739GGLY46.105752050.7868831231.9791.312C0
5740TTHR42.818863880.5977234112.0871.757C0
5741LLEU47.677742050.8773508121.1981.497C0
5742EGLU31.4933905-0.0540551620.7931.266T0
5743PPRO60.271525541.6021203610.7920.865T0
5744EGLU39.389067520.4003393641.1861.287T0
5745YTYR10.64665952-1.2537800760.9431.067T0
5746FPHE18.57806359-0.7973294660.7320.752C0
5747NASN45.480528760.7509016560.7980.845H0
5748SSER13.73704895-1.0759288250.7190.797H0
5749VVAL58.467309751.4982881280.6321.146H0
5750CCYS4.251518399-1.6218190810.660.969H0
5751RARG18.27438371-0.8148061780.7891.484H0
5752LLEU39.567702750.4106197830.7761.426H0
5753MMET45.069667740.7272566920.7941.139H0
5754KLYS5.680600214-1.5395757281.3061.422H0
5755TTHR6.788138621-1.4758371291.2681.478H0
5756IILE8.985351911-1.3493879741.3121.62H0
5757GGLY28.54590925-0.2236820780.8980.849C0
5758PPRO42.83672740.5987514530.9330.848C0
5759DASP46.016434440.7817429140.9530.819C0
5760MMET4.573061808-1.6033143261.0440.918E0
5761FPHE44.748124330.7087519381.1790.887E0
5762LLEU47.302608070.8557619311.3571.001E0
5763GGLY10.7895677-1.245555741.3730.932E0
5764TTHR13.57627724-1.0851812021.2661.118E0
5765CCYS44.658806720.7036117281.1440.821E1
5766RARG3.929974991-1.6403238350.9721.514C1
5767RARG46.605930690.8156682972.4972.299C1
5768CCYS44.748124330.7087519381.710.965C2
5769PPRO47.963558410.8937994821.3881.168H1
5770AALA20.471597-0.6883570231.5581.186H0
5771EGLU45.176848870.7334249441.2481.022H0
5772IILE44.033583420.6676302611.0891.085H0
5773VVAL45.659163990.7611820751.3921.005H0
5774DASP22.41872097-0.5763004542.0991.211H0
5775TTHR45.409074670.7467894891.6810.953H0
5776VVAL46.820292960.82800481.1840.955H0
5777SSER44.80171490.7118360641.3681.051H0
5778AALA24.24080029-0.4714401791.2971.348H0
5779LLEU34.708824580.1309923821.251.401C0
5780VVAL45.659163990.7611820751.0151.265C0
5781YTYR44.783851380.7108080221.2990.751T0
5782DASP18.41729189-0.8065818431.5361.172T0
5783NASN38.227938550.3335166391.8961.357T0
5784KLYS34.244372990.1042632922.0511.459T0
5785LLEU23.09753483-0.5372348621.4540.975C0
5786KLYS14.70167917-1.0204145611.2331.89E0
5787AALA28.20650232-0.2432148741.1011.133C0
5788HHIS3.733476242-1.6516322961.3161.53C0
5789KLYS17.68488746-0.8487315622.0731.75C1
5790DASP5.180421579-1.5683609012.0721.793C2
5791KLYS4.269381922-1.6207910391.8012.213C1
5792SSER44.712397280.7066958541.3251.483C0
5793AALA27.20614505-0.3007852221.761.537C0
5794QGLN65.880671671.9249255221.4551.758E1
5795CCYS44.837441940.7138921471.2871.093E2
5796FPHE43.569131830.6409011711.3831.431E2
5797KLYS46.498749550.8095000451.3851.254E2
5798MMET6.341550554-1.5015381771.7291.805E3
5799FPHE18.63165416-0.794245342.2741.682E4
5800YTYR17.20257235-0.8764886932.4991.913E6
5801KLYS33.690603790.0723939932.7682.66C5
5802GGLY35.155412650.156693432.6822.062C6
5803VVAL8.663808503-1.3678927282.8062.763C1
5804IILE4.215791354-1.6238751652.5972.525C0
5805TTHR18.56020007-0.7983575082.9223.33E0
5806HHIS28.06359414-0.251439215.5754.143C1
5807DASP26.56305824-0.337794736.2454.497C2
5808VVAL3.965702036-1.6382677515.283.238C1
5809SSER18.63165416-0.794245344.5533.178C0
5810SSER44.730260810.7077238962.4192.287C0
5811AALA28.15291175-0.2462993.1772.253C3
5812IILE27.68846016-0.273028092.9312.259E6
5813NASN44.676670240.704639772.812.04H5
5814RARG25.59842801-0.3933089942.842.581H7
5815PPRO12.45087531-1.1499478422.4511.934H6
5816QGLN44.640943190.7025836862.2111.816H7
5817IILE10.73597713-1.2486398662.7351.772H6
5818GGLY4.32297249-1.6177069132.5271.603H7
5819VVAL20.66809575-0.6770485622.0631.705H5
5820VVAL38.067166850.3242642622.2131.46H6
5821RARG16.09503394-0.9402272922.3052.134H6
5822EGLU4.644515899-1.5992021592.2741.787H6
5823FPHE44.926759560.7190323572.0391.43H7
5824LLEU35.869953550.1978151072.0581.899H6
5825TTHR13.8620936-1.0687325312.2111.783H5
5826RARG11.59342622-1.1992938542.1972.303H4
5827NASN44.194355130.6768826382.1731.539H6
5828PPRO34.708824580.1309923822.3851.814T3
5829AALA11.36120043-1.2126583993.0281.76T2
5830WTRP44.748124330.7087519382.5391.344T4
5831RARG13.41550554-1.0944335792.5422.177T1
5832KLYS33.38692390.0549172812.4463.446C0
5833AALA42.729546270.5925832021.8561.424C1
5834VVAL34.994640940.1474410531.7411.596E2
5835FPHE44.837441940.7138921471.7431.204E3
5836IILE44.980350130.7221164831.6171.329E2
5837SSER45.212575920.7354810281.8061.258C7
5838PPRO45.158985350.7323969022.1521.519C4
5839YTYR45.123258310.7303408181.9841.668H8
5840NASN45.659163990.7611820752.0321.953H8
5841SSER35.619864240.183422522.2241.827H8
5842QGLN36.370132190.226600282.1741.67H10
5843NASN44.748124330.7087519381.8181.413H9
5844AALA11.84351554-1.1849012672.3441.792H8
5845VVAL29.93926402-0.1434948092.5741.983H8
5846AALA44.623079670.7015556442.081.614H8
5847SSER17.63129689-0.8518156872.4761.776H7
5848KLYS25.61629153-0.3922809523.132.659H7
5849IILE4.841014648-1.5878936982.9961.953H6
5850LLEU43.85494820.6573498422.3991.696H6
5851GGLY84.72668813.0095097412.9661.703C5
5852LLEU42.568774560.5833308242.1891.61C3
5853PPRO3.733476242-1.6516322961.9191.47E2
5854TTHR25.09824937-0.4220941671.8631.574E1
5855QGLN46.266523760.79613551.9381.835E1
5856TTHR45.033940690.7252006081.9491.315H1
5857VVAL64.576634511.8498784622.6351.587H0
5858DASP44.837441940.7138921472.1031.512H0
5859SSER44.80171490.7118360642.3111.398H0
5860SSER62.504465881.73062563.3481.14H0
5861QGLN44.891032510.7169762732.3841.63H0
5862GGLY44.998213650.7231445252.4131.207C0
5863SSER44.873168990.7159482311.7881.318C0
5864EGLU44.783851380.7108080221.5921.457C1
5865YTYR44.319399790.6840789321.6551.373E2
5866DASP36.906037870.2574415381.6661.285C2
5867YTYR44.301536260.683050891.7881.579E2
5868VVAL49.83922831.0017438831.6181.388E2
5869IILE45.19471240.7344529861.8651.245E2
5870FPHE22.81171847-0.5536835321.9431.264E1
5871TTHR17.91711325-0.8353670171.831.442E2
5872QGLN35.226866740.1608055981.9791.742C2
5873TTHR46.230796710.7940794171.9941.627C1
5874TTHR6.234369418-1.5077064282.5331.921C0
5875EGLU11.59342622-1.1992938542.2461.861C2
5876TTHR35.334047870.1669738492.5642.205T2
5877AALA34.762415150.1340765082.3652.219T2
5878HHIS44.783851380.7108080221.8243.024T0
5879SSER10.7895677-1.245555741.5781.489T0
5880CCYS16.47016792-0.9186384121.6091.36C0
5881NASN54.340836011.2608104462.091.126H0
5882VVAL25.59842801-0.3933089941.5241.33H0
5883NASN70.757413362.2055809641.2350.83H0
5884RARG84.030010722.9694161061.6921.441H0
5885FPHE46.105752050.7868831231.4831.008H0
5886NASN85.226866743.0382949141.1080.659H0
5887VVAL86.441586283.1082017651.3760.948H0
5888AALA81.939978562.8491352021.4260.917H0
5889IILE54.287245441.257726321.1731.072H0
5890TTHR56.430868171.3810913491.2550.829H0
5891RARG86.262951053.0979213461.2011.552E0
5892AALA79.456948912.7062373761.2431.02E0
5893KLYS54.715969991.2823993261.4881.354E1
5894VVAL5.644873169-1.5416318121.5481.366C2
5895GGLY47.338335120.8578180151.541.082C2
5896IILE44.998213650.7231445251.3341.105E2
5897LLEU44.087173990.6707143871.4991.242E1
5898CCYS35.441229010.1731421011.3541.173E1
5899IILE24.11575563-0.4786364731.7821.576E5
5900MMET42.943908540.6049197041.7971.406E6
5901SSER30.65380493-0.1023731322.1981.779C5
5902DASP20.81100393-0.6688242272.0821.639C5
5903RARG28.65309039-0.2175138272.0772.503C4
5904DASP4.019292605-1.6351836262.0731.929H3
5905LLEU42.425866380.5751064892.451.695H3
5906YTYR16.93461951-0.8919093222.0871.395H3
5907DASP20.65023223-0.6780766041.9291.648H2
5908KLYS4.32297249-1.6177069131.9161.589H2
5909LLEU46.463022510.8074439621.7431.531H2
5910QGLN11.20042872-1.2219107761.6781.451C2
5911FPHE44.730260810.7077238961.3811.023C2
5912TTHR23.1511254-0.5341507361.5421.731C1
5913SSER5.78778135-1.5334074762.4771.872T1
5914LLEU38.888888890.371554192.5482.557T1
5915EGLU3.929974991-1.6403238352.8382.423T1
5916IILE2.715255448-1.7102306854.3612.303T1
5917PPRO3.840657378-1.6454640457.7172.439T0
5918RARG3.912111468-1.6413518776.7063.592T0
5919RARG3.679885673-1.6547164224.6083.551T1
5920NASN4.32297249-1.6177069134.9552.724T1
5921VVAL4.126473741-1.6290153742.7241.897C1
5922AALA3.751339764-1.6506042542.8572.065C1
5923TTHR4.305108967-1.6187349552.3321.998C0
5924LLEU5.05537692-1.5755571953.4663.451C0
5925QGLN4.305108967-1.6187349556.2295.956C0

2.            I-TASSER server for protein structure and function prediction [Internet]. [cited 2020 Apr 12];Available from: https://zhanglab.ccmb.med.umich.edu/I-TASSER/

23.         Shu T, Huang M, Wu D, et al. SARS-coronavirus-2 nsp13 possesses NTPase and RNA helicase activities.

24.         Mirza MU, Froeyen M. Structural Elucidation of SARS-CoV-2 Vital Proteins: Computational Methods Reveal Potential Drug Candidates Against Main Protease, Nsp12 RNA-dependent RNA Polymerase and Nsp13 Helicase. 2020 [cited 2020 Apr 9];Available from: https://www.preprints.org/manuscript/202003.0085/v1