3C-like proteinase Google Drive Data

Cysteine-like protease (3CL^pro) (M^pro) (nsp5)– the 3D structure of 3CL^pro of SARS-CoV-2 is very similar to SARS-CoV with a sequence identity of >96%.⁷ 3CL^pro is responsible for all cleavage events not mediated by PL^pro, it cleaves nsp4-16. It recognizes substrates containing the core sequence [ILMVF]-Q-|-[SGACN]. The protease is also able to bind an ADP-ribose-1”-phosphate (ADRP).² 3CL^pro also interferes with localization of some proteins. Wild-type 3CL^pro inhibits histone deacetylase 2 (HDAC2) transport into the nucleus, potentially impacting mediation of inflammation and interferon response. 3CL^pro (C145A) interferes with tRNA methyltransferase 1 (TRMT1). TRMT1 is cleaved by 3CL^pro, removing its zinc finger and nuclear localization signal and likely resulting in an exclusively mitochondrial localization. TRMT1 is responsible for synthesis of the dimethylguanosine (m2,2G) base modification on both nuclear and mitochondrial tRNAs40.⁸ 3CL^pro is a promising target for anti-coronaviral drugs. One study found that Cobicistat and Amprenavir bind to the protease effectively. Amprenavir’s prodrug version is called Fosamprenavir, and is currently used in treating HIV. The study recommended Fosamprenavir be considered as a potential treatment for SARS-CoV-2.⁹ Another study identified compound 621 from Asinex Focused Covalent Library as well as Paritaprevir and Simeprevir from FDA-approved protease inhibitors as potential inhibitors of 3CL^pro. ¹⁰ Antivirals ledipasvir and velpatasvir are attractive therapeutics as well. Based on an in silico study, 2-deoxy-D-glucose can inactivate 3CL^pro and nsp15 endoribonuclease.¹¹

3C-like proteinase 3,397 Sequences

Amino Acid	AA	AA	Conservation	Conservation Z-score	RMSD_CA	RMSF[A]	Secondary Structure	DCCM >0.9
3264	S	SER	56.55375552	0.192178888	2.749	1.574	C	0
3265	G	GLY	99.73490427	1.433066696	2.351	0.967	C	0
3266	F	PHE	25.09572901	-0.711823963	2.078	0.96	C	0
3267	R	ARG	37.58468336	-0.352931445	2.866	0.844	C	1
3268	K	LYS	95.40500736	1.308639338	3.275	1.06	C	1
3269	M	MET	78.910162	0.834630352	3.766	1.325	C	0
3270	A	ALA	44.00589102	-0.168406519	2.337	0.899	C	0
3271	F	PHE	11.51693667	-1.102034931	1.044	1.732	C	0
3272	P	PRO	99.79381443	1.434759586	1.571	1.329	C	0
3273	S	SER	85.65537555	1.028466169	1.719	1.449	C	0
3274	G	GLY	70.27982327	0.58662208	2.586	1.636	C	0
3275	K	LYS	23.00441826	-0.77192153	2.697	2.386	C	0
3276	V	VAL	97.64359352	1.372969129	2.407	1.662	C	0
3277	E	GLU	99.82326951	1.43560603	2.23	1.229	C	0
3278	G	GLY	10.0736377	-1.143510717	2.218	0.943	C	0
3279	C	CYS	99.73490427	1.433066696	1.932	0.923	E	0
3280	M	MET	35.52282769	-0.412182568	1.641	0.867	E	0
3281	V	VAL	99.76435935	1.433913141	1.403	0.983	E	0
3282	Q	GLN	32.57731959	-0.49682703	1.446	1.273	E	1
3283	V	VAL	99.73490427	1.433066696	1.159	1.081	E	2
3284	T	THR	47.27540501	-0.074451166	1.336	1.134	E	2
3285	C	CYS	32.69513991	-0.493441252	1.702	1.215	E	1
3286	G	GLY	80.11782032	0.869334582	4.04	1.525	T	0
3287	T	THR	9.484536082	-1.16043961	3.529	1.953	T	0
3288	T	THR	17.5257732	-0.929360229	1.749	1.483	E	1
3289	T	THR	49.07216495	-0.022818045	1.474	1.277	E	2
3290	L	LEU	99.82326951	1.43560603	1.25	1.523	E	1
3291	N	ASN	99.82326951	1.43560603	1.142	0.776	E	0
3292	G	GLY	99.73490427	1.433066696	1.106	0.723	E	0
3293	L	LEU	90.57437408	1.16982242	1.257	0.898	E	1
3294	W	TRP	99.85272459	1.436452475	1.171	0.833	E	1
3295	L	LEU	98.46833579	1.396669578	1.63	1.009	E	0
3296	D	ASP	48.07069219	-0.051597162	1.422	1.277	T	0
3297	D	ASP	71.45802651	0.620479864	1.196	1.101	T	0
3298	V	VAL	6.715758468	-1.240005404	1.135	1.213	E	0
3299	V	VAL	84.62444772	0.998840608	0.77	0.958	E	0
3300	Y	TYR	48.12960236	-0.049904272	0.812	0.876	E	0
3301	C	CYS	99.55817378	1.427988029	0.937	1.007	E	0
3302	P	PRO	99.85272459	1.436452475	0.856	0.983	C	0
3303	R	ARG	99.85272459	1.436452475	1.333	1.417	G	0
3304	H	HIS	99.82326951	1.43560603	1.865	2.13	G	1
3305	V	VAL	98.88070692	1.408519802	1.316	1.482	G	1
3306	I	ILE	58.05596465	0.235347564	1.598	1.642	G	0
3307	C	CYS	46.92194404	-0.084608502	1.814	1.83	C	1
3308	T	THR	9.337260677	-1.164671833	2.584	2.099	T	2
3309	S	SER	25.56701031	-0.698280849	2.626	2.262	T	2
3310	E	GLU	9.366715758	-1.163825388	2.285	2.384	T	2
3311	D	ASP	21.79675994	-0.80662576	1.847	1.826	T	1
3312	M	MET	23.03387334	-0.771075086	1.987	2.46	C	0
3313	L	LEU	12.81296024	-1.064791368	1.917	2.53	C	0
3314	N	ASN	20.14727541	-0.854026658	2.567	2.896	C	0
3315	P	PRO	46.9808542	-0.082915612	2.857	3.233	C	0
3316	N	ASN	32.57731959	-0.49682703	3.468	2.939	H	0
3317	Y	TYR	80.20618557	0.871873915	2.964	2.66	H	1
3318	E	GLU	14.93372607	-1.003847355	3.195	3.158	H	1
3319	D	ASP	25.03681885	-0.713516852	2.545	2.324	H	0
3320	L	LEU	46.95139912	-0.083762057	2.446	2.153	H	0
3321	L	LEU	82.65095729	0.942128819	2.117	2.266	H	0
3322	I	ILE	31.7820324	-0.519681035	2.167	2.195	H	0
3323	R	ARG	23.62297496	-0.754146194	2.213	3.083	H	1
3324	K	LYS	10.16200295	-1.140971383	2.223	1.802	C	1
3325	S	SER	9.484536082	-1.16043961	2.164	1.671	T	0
3326	N	ASN	49.01325479	-0.024510934	1.558	1.34	T	1
3327	H	HIS	78.55670103	0.824473017	1.698	2.007	T	2
3328	N	ASN	34.87481591	-0.43080435	2.048	1.449	T	1
3329	F	PHE	99.5287187	1.427141584	1.333	1.329	C	0
3330	L	LEU	9.278350515	-1.166364722	1.233	1.576	E	0
3331	V	VAL	69.33726068	0.559535852	1.119	1.235	E	0
3332	Q	GLN	32.60677467	-0.495980585	1.799	1.377	E	0
3333	A	ALA	9.690721649	-1.154514497	1.629	1.235	E	0
3334	G	GLY	41.14874816	-0.250511647	2.361	1.77	T	0
3335	N	ASN	50.78055965	0.026275743	3.57	3.205	T	0
3336	V	VAL	50.78055965	0.026275743	2.146	1.936	E	0
3337	Q	GLN	13.04860088	-1.058019811	1.997	2.172	E	0
3338	L	LEU	99.23416789	1.418677138	1.209	1.23	E	0
3339	R	ARG	33.28424153	-0.476512359	1.165	2.001	E	1
3340	V	VAL	99.46980854	1.425448695	1.11	1.298	E	2
3341	I	ILE	11.75257732	-1.095263374	1.326	1.305	E	2
3342	G	GLY	57.40795287	0.216725782	1.35	1.163	E	3
3343	H	HIS	33.28424153	-0.476512359	1.276	1.507	E	2
3344	S	SER	11.95876289	-1.089338262	1.295	1.479	E	2
3345	M	MET	78.46833579	0.821933683	1.424	1.835	E	1
3346	Q	GLN	40.97201767	-0.255590314	2.126	1.969	E	1
3347	N	ASN	9.278350515	-1.166364722	1.885	1.359	T	2
3348	C	CYS	22.06185567	-0.799007758	1.227	1.109	T	2
3349	V	VAL	20.4712813	-0.844715768	1.019	1.06	E	1
3350	L	LEU	99.67599411	1.431373807	1.002	1.644	E	1
3351	K	LYS	29.27835052	-0.591628827	1.034	1.531	E	1
3352	L	LEU	71.84094256	0.631483644	1.034	1.209	E	1
3353	K	LYS	41.00147275	-0.25474387	1.098	1.67	E	3
3354	V	VAL	84.47717231	0.994608385	0.929	1.102	E	2
3355	D	ASP	33.10751105	-0.481591027	1.151	1.402	C	1
3356	T	THR	13.25478645	-1.052094699	1.179	1.461	C	0
3357	A	ALA	38.20324006	-0.335156108	1.212	1.243	C	1
3358	N	ASN	99.91163476	1.438145364	1.249	1.021	T	1
3359	P	PRO	42.50368189	-0.211575194	1.413	1.415	T	0
3360	K	LYS	10.04418262	-1.144357162	3.427	2.875	T	0
3361	T	THR	99.91163476	1.438145364	2.486	1.163	T	0
3362	P	PRO	99.94108984	1.438991809	2.56	1.086	C	0
3363	K	LYS	59.05743741	0.264126681	2.5	1.607	E	1
3364	Y	TYR	86.80412371	1.06147751	2.107	1.071	E	2
3365	K	LYS	34.37407953	-0.445193908	1.927	1.498	E	2
3366	F	PHE	81.8556701	0.919274814	1.729	1.302	E	2
3367	V	VAL	17.23122239	-0.937824675	1.8	1.439	E	1
3368	R	ARG	13.69661267	-1.039398029	1.825	1.362	C	0
3369	I	ILE	11.7820324	-1.094416929	1.585	1.133	C	1
3370	Q	GLN	12.66568483	-1.069023591	1.396	1.397	T	1
3371	P	PRO	75.11045655	0.725438997	1.223	1.104	T	0
3372	G	GLY	99.88217968	1.437298919	1.179	1.03	T	1
3373	Q	GLN	9.837997054	-1.150282274	1.344	1.453	E	1
3374	T	THR	23.44624448	-0.759224861	1.545	1.281	E	0
3375	F	PHE	95.46391753	1.310332227	1.216	0.794	E	0
3376	S	SER	33.60824742	-0.467201468	1.085	0.849	E	0
3377	V	VAL	46.48011782	-0.097305171	0.93	1.168	E	0
3378	L	LEU	80.29455081	0.874413249	1.171	1.176	E	0
3379	A	ALA	79.85272459	0.86171658	1.274	0.881	E	0
3380	C	CYS	66.15611193	0.468119833	1.561	0.924	E	0
3381	Y	TYR	99.94108984	1.438991809	1.536	0.837	E	0
3382	N	ASN	47.06921944	-0.080376279	1.878	1.468	T	0
3383	G	GLY	99.88217968	1.437298919	1.638	1.073	T	0
3384	S	SER	21.97349043	-0.801547092	1.91	0.976	E	0
3385	P	PRO	55.28718704	0.15578177	1.72	0.917	E	0
3386	S	SER	14.34462445	-1.020776248	2.013	0.944	E	0
3387	G	GLY	90.86892489	1.178286867	1.299	0.731	E	0
3388	V	VAL	47.7466863	-0.060908052	1.094	0.92	E	0
3389	Y	TYR	56.75994109	0.198104	0.932	0.817	E	0
3390	Q	GLN	9.307805596	-1.165518277	1.139	1.132	E	0
3391	C	CYS	9.896907216	-1.148589385	1.187	0.945	E	0
3392	A	ALA	9.366715758	-1.163825388	0.901	0.952	E	0
3393	M	MET	88.24742268	1.102953296	0.659	0.972	C	0
3394	R	ARG	95.93519882	1.323875341	0.759	0.747	T	0
3395	P	PRO	32.04712813	-0.512063033	1.121	0.924	T	0
3396	N	ASN	79.20471281	0.843094798	0.837	0.79	T	0
3397	F	PHE	5.449189985	-1.276402522	0.786	0.954	T	0
3398	T	THR	95.90574374	1.323028896	0.795	0.813	C	0
3399	I	ILE	98.52724595	1.398362467	0.831	0.87	C	0
3400	K	LYS	57.58468336	0.22180445	0.993	1.082	C	0
3401	G	GLY	79.97054492	0.865102358	1.255	0.833	T	0
3402	S	SER	99.76435935	1.433913141	1.32	0.993	T	0
3403	F	PHE	99.82326951	1.43560603	1.195	1.204	T	0
3404	L	LEU	66.3622975	0.474044945	1.07	0.874	T	0
3405	N	ASN	39.82326951	-0.288601654	2.258	1.179	C	0
3406	G	GLY	99.82326951	1.43560603	3.899	0.991	C	0
3407	S	SER	47.86450663	-0.057522274	3.378	0.754	C	0
3408	C	CYS	99.67599411	1.431373807	1.108	0.689	C	0
3409	G	GLY	99.88217968	1.437298919	1.107	0.557	E	0
3410	S	SER	99.76435935	1.433913141	1.82	0.812	E	0
3411	V	VAL	68.86597938	0.545992738	1.455	1.174	E	0
3412	G	GLY	99.70544919	1.432220252	1.081	0.615	E	0
3413	F	PHE	26.9808542	-0.657651507	1.167	0.897	E	0
3414	N	ASN	50.25036819	0.01103974	2.025	1.258	E	1
3415	I	ILE	34.87481591	-0.43080435	2.802	1.724	E	2
3416	D	ASP	9.513991163	-1.159593165	3.019	1.819	E	2
3417	Y	TYR	9.307805596	-1.165518277	3.945	3.06	T	2
3418	D	ASP	26.68630339	-0.666115953	3.887	1.95	T	1
3419	C	CYS	19.85272459	-0.862491104	2.463	1.455	E	1
3420	V	VAL	67.09867452	0.495206061	2.112	1.41	E	1
3421	S	SER	9.337260677	-1.164671833	1.692	1.106	E	0
3422	F	PHE	95.75846834	1.318796673	1.295	0.805	E	0
3423	C	CYS	55.43446244	0.160013993	1.146	1.005	E	0
3424	Y	TYR	99.76435935	1.433913141	1.08	0.753	E	0
3425	M	MET	77.34904271	0.789768788	0.961	0.886	E	0
3426	H	HIS	99.70544919	1.432220252	0.681	0.64	E	0
3427	H	HIS	35.8173785	-0.403718122	0.694	0.839	E	0
3428	M	MET	33.10751105	-0.481591027	0.834	1.185	E	0
3429	E	GLU	99.73490427	1.433066696	1.092	1.093	E	0
3430	L	LEU	95.61119293	1.31456445	1.081	1.113	E	1
3431	P	PRO	25.68483063	-0.69489507	1.527	1.313	T	1
3432	T	THR	23.06332842	-0.770228641	1.272	1.175	T	0
3433	G	GLY	78.70397644	0.82870524	1.462	1.17	E	0
3434	V	VAL	9.837997054	-1.150282274	0.925	1.182	E	0
3435	H	HIS	99.61708395	1.429680918	1.008	0.91	E	0
3436	A	ALA	9.307805596	-1.165518277	0.773	0.822	E	0
3437	G	GLY	99.76435935	1.433913141	0.682	0.651	E	0
3438	T	THR	43.00441826	-0.197185636	0.856	0.789	E	0
3439	D	ASP	66.5095729	0.478277169	1.429	0.904	E	0
3440	L	LEU	49.95581738	0.002575294	1.659	1.346	T	0
3441	E	GLU	18.79234168	-0.892963111	1.86	1.547	T	0
3442	G	GLY	99.67599411	1.431373807	1.557	0.898	E	0
3443	N	ASN	11.34020619	-1.107113599	1.458	1.248	E	1
3444	F	PHE	43.29896907	-0.188721189	1.109	0.965	E	1
3445	Y	TYR	99.76435935	1.433913141	0.824	0.789	C	0
3446	G	GLY	99.76435935	1.433913141	0.859	0.852	C	0
3447	P	PRO	27.33431517	-0.647494172	1.482	1.072	C	0
3448	F	PHE	41.91458027	-0.228504086	1.293	0.909	C	0
3449	V	VAL	22.68041237	-0.781232421	1.538	1.381	C	0
3450	D	ASP	99.79381443	1.434759586	2.069	1.246	C	0
3451	R	ARG	10.51546392	-1.130814048	2.759	2.387	C	0
3452	Q	GLN	46.95139912	-0.083762057	2.38	2.342	C	1
3453	T	THR	28.42415317	-0.616175721	2.182	2.005	C	2
3454	A	ALA	25.24300442	-0.70759174	2.271	1.919	C	1
3455	Q	GLN	99.82326951	1.43560603	1.969	1.113	C	0
3456	A	ALA	9.985272459	-1.146050051	1.539	1.217	T	0
3457	A	ALA	10.04418262	-1.144357162	2.189	1.502	T	0
3458	G	GLY	36.4359352	-0.385942785	1.873	1.432	T	0
3459	T	THR	37.0544919	-0.368167448	1.32	1.053	T	0
3460	D	ASP	63.15169367	0.381782482	1.09	1.357	C	0
3461	T	THR	10.63328424	-1.12742827	1.435	1.216	C	0
3462	T	THR	11.19293078	-1.111345822	2.143	1.052	E	0
3463	I	ILE	12.51840943	-1.073255814	2.514	1.3	H	1
3464	T	THR	58.61561119	0.251430011	2.42	0.94	H	1
3465	V	VAL	41.97349043	-0.226811197	2.61	1.094	H	1
3466	N	ASN	99.79381443	1.434759586	2.53	0.761	H	1
3467	V	VAL	72.78350515	0.658569872	1.982	0.905	H	0
3468	L	LEU	38.35051546	-0.330923885	1.897	0.979	H	0
3469	A	ALA	98.40942563	1.394976689	2.199	0.868	H	0
3470	W	TRP	63.91752577	0.403790042	2.041	0.895	H	0
3471	L	LEU	97.93814433	1.381433575	1.697	0.872	H	0
3472	Y	TYR	99.64653903	1.430527362	2.145	0.772	H	1
3473	A	ALA	96.78939617	1.348422235	2.576	0.946	H	1
3474	A	ALA	95.69955817	1.317103784	2.221	0.928	H	1
3475	V	VAL	10.81001473	-1.122349602	2.518	1.104	H	2
3476	I	ILE	49.83799705	-0.000810485	3.213	1.218	H	2
3477	N	ASN	79.67599411	0.856637912	3.526	1.259	H	1
3478	G	GLY	66.39175258	0.47489139	3.353	1.211	C	0
3479	D	ASP	14.28571429	-1.022469137	2.641	1.185	C	0
3480	R	ARG	20.14727541	-0.854026658	3.021	1.851	T	0
3481	W	TRP	79.97054492	0.865102358	3.005	1.437	T	0
3482	F	PHE	52.90132548	0.087219756	2.004	0.99	T	0
3483	L	LEU	54.78645066	0.141392211	1.688	1.17	T	0
3484	N	ASN	9.867452135	-1.14943583	1.39	1.694	C	0
3485	R	ARG	9.337260677	-1.164671833	1.926	2.824	C	0
3486	F	PHE	9.307805596	-1.165518277	2.024	2.398	C	0
3487	T	THR	24.62444772	-0.725367077	2.244	1.61	C	1
3488	T	THR	32.54786451	-0.497673475	2.235	1.368	C	2
3489	T	THR	13.40206186	-1.047862476	2.347	1.42	H	2
3490	L	LEU	17.82032401	-0.920895783	2.719	1.599	H	2
3491	N	ASN	9.572901325	-1.157900276	3.14	1.84	H	2
3492	D	ASP	41.11929308	-0.251358091	2.794	1.517	H	2
3493	F	PHE	73.54933726	0.680577432	2.425	1.346	H	2
3494	N	ASN	99.76435935	1.433913141	2.843	1.348	H	2
3495	L	LEU	9.307805596	-1.165518277	3.064	2.148	H	1
3496	V	VAL	9.307805596	-1.165518277	2.516	1.912	H	0
3497	A	ALA	98.910162	1.409366247	2.521	1.351	H	0
3498	M	MET	16.34756996	-0.963218014	3.241	2.437	H	1
3499	K	LYS	9.926362297	-1.14774294	2.892	2.46	H	1
3500	Y	TYR	10.0736377	-1.143510717	1.986	1.693	C	0
3501	N	ASN	9.837997054	-1.150282274	3.143	1.957	E	0
3502	Y	TYR	13.10751105	-1.056326922	2.55	1.267	E	1
3503	E	GLU	9.337260677	-1.164671833	2.431	1.267	E	1
3504	P	PRO	19.23416789	-0.880266441	2.552	1.223	C	0
3505	L	LEU	14.93372607	-1.003847355	2.841	1.518	C	0
3506	T	THR	10.30927835	-1.13673916	3.472	1.351	H	1
3507	Q	GLN	9.307805596	-1.165518277	3.821	1.965	H	2
3508	D	ASP	9.602356406	-1.157053831	4.251	1.489	H	2
3509	H	HIS	9.278350515	-1.166364722	3.367	1.1	H	2
3510	V	VAL	9.307805596	-1.165518277	2.929	1.23	H	2
3511	D	ASP	39.02798233	-0.311455659	3.632	1.431	H	2
3512	I	ILE	9.189985272	-1.168904056	3.416	1.359	H	2
3513	L	LEU	13.75552283	-1.03770514	3.041	0.991	H	2
3514	G	GLY	9.572901325	-1.157900276	3.347	1.038	H	2
3515	P	PRO	9.985272459	-1.146050051	3.685	1.221	H	2
3516	L	LEU	98.61561119	1.400901801	3.041	1.137	H	2
3517	S	SER	28.77761414	-0.606018386	2.432	1.058	H	2
3518	A	ALA	58.17378498	0.238733342	3.029	1.353	H	2
3519	Q	GLN	9.13107511	-1.170596945	3.359	1.598	H	2
3520	T	THR	96.08247423	1.328107564	2.697	1.178	H	2
3521	G	GLY	95.31664212	1.306100004	2.213	1.179	H	2
3522	I	ILE	10.19145803	-1.140124939	1.541	1.285	C	1
3523	A	ALA	30.95729013	-0.543381484	1.544	1.104	H	0
3524	V	VAL	64.88954345	0.431722715	1.765	1.049	H	0
3525	L	LEU	9.307805596	-1.165518277	1.685	1.205	H	0
3526	D	ASP	9.248895434	-1.167211167	1.473	0.964	H	1
3527	M	MET	10.81001473	-1.122349602	1.421	0.869	H	1
3528	C	CYS	9.366715758	-1.163825388	1.679	0.849	H	1
3529	A	ALA	46.65684831	-0.092226503	1.667	1.096	H	2
3530	S	SER	25.24300442	-0.70759174	1.468	1.018	H	1
3531	L	LEU	10.0736377	-1.143510717	1.464	1.2	H	1
3532	K	LYS	23.97643594	-0.743988858	2.106	1.695	H	1
3533	E	GLU	8.483063328	-1.189218727	2.335	1.551	H	1
3534	L	LEU	83.4167894	0.964136379	2.236	1.768	H	2
3535	L	LEU	9.955817378	-1.146896496	2.454	1.844	H	2
3536	Q	GLN	10.04418262	-1.144357162	4.078	2.773	H	1
3537	N	ASN	9.307805596	-1.165518277	4.257	1.971	H	0
3538	G	GLY	61.50220913	0.334381584	2.701	1.322	C	0
3539	M	MET	9.337260677	-1.164671833	2.399	1.667	C	0
3540	N	ASN	9.602356406	-1.157053831	2.038	1.252	C	0
3541	G	GLY	83.65243004	0.970907936	1.297	0.844	C	0
3542	R	ARG	28.27687776	-0.620407944	1.928	1.258	C	0
3543	T	THR	18.05596465	-0.914124226	2.555	1.156	C	0
3544	I	ILE	98.85125184	1.407673358	1.986	1.028	T	0
3545	L	LEU	84.53608247	0.996301274	2.307	1.095	T	0
3546	G	GLY	94.49189985	1.282399555	2.301	0.913	T	0
3547	S	SER	49.66126657	-0.005889152	2.368	1.155	T	0
3548	A	ALA	3.858615611	-1.322110531	3.498	0.955	C	0
3549	L	LEU	1.973490427	-1.376282987	1.994	1.882	C	0
3550	L	LEU	67.77614138	0.514674287	1.256	1.295	C	0
3551	E	GLU	65.94992636	0.462194721	1.398	1.235	C	0
3552	D	ASP	95.75846834	1.318796673	2.068	0.777	C	0
3553	E	GLU	95.61119293	1.31456445	2.781	1.007	C	0
3554	F	PHE	63.97643594	0.405482932	3.597	0.921	C	0
3555	T	THR	94.69808542	1.288324667	4.005	1.038	H	0
3556	P	PRO	71.7820324	0.629790755	3.89	0.955	H	1
3557	F	PHE	9.42562592	-1.162132499	4.177	1.429	H	2
3558	D	ASP	46.92194404	-0.084608502	4.067	1.032	H	2
3559	V	VAL	98.26215022	1.390744465	3.463	1.123	H	2
3560	V	VAL	19.76435935	-0.865030438	3.883	1.387	H	2
3561	R	ARG	33.46097202	-0.471433691	4.287	2.063	H	2
3562	Q	GLN	99.41089838	1.423755806	4.082	1.374	H	2
3563	C	CYS	9.42562592	-1.162132499	5.552	1.593	C	1
3564	S	SER	9.661266568	-1.155360942	6.528	1.983	C	0
3565	G	GLY	95.58173785	1.313718005	5.316	1.752	C	0
3566	V	VAL	84.74226804	1.002226386	6.578	2.566	C	0
3567	T	THR	10.98674521	-1.117270934	8.7	4.059	C	1
3568	F	PHE	8.983799705	-1.174829168	11.571	5.853	C	2
3569	Q	GLN	94.81590574	1.291710445	12.904	6.502	C	1

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