2′-O-methyltransferase Google Drive Data

Gray amino acids fall below the average conservation (value <0) for each protein, yellow = 0-0.5, light orange = 0.5-1, orange = 1-1.5, dark orange = 1.5-2, red = >2

2′-O-methyltransferase (2′-O-MT) (nsp 16)– mediates mRNA cap 2′-O-ribose methylation to the 5′-cap structure of viral mRNAs. N7-methyl guanosine cap is required for binding of nsp16. This methylation helps the virus evade the host immune system as it shields viral RNA from MDA5 melanoma differentiation associated protein 5 (MDA5) recognition.2,27 2′-O-MT interacts with nsp10.4

nsp16 interacting with nsp10 showing conserved contacts

2′-O-methyltransferase 2,495 Sequences

Amino AcidAAAAConservationConservation Z-scoreRMSD_CARMSF[A]Secondary StructureDCCM >0.9
6799SSER2.965931864-1.6949579955.0953.633T0
6800SSER39.3987976-0.6285149033.9342.518T0
6801QGLN13.58717435-1.3840587443.5312.341T0
6802AALA24.48897796-1.0649470592.5851.526T0
6803WTRP95.190380761.004586072.2841.71C0
6804QGLN8.336673347-1.5377485621.7431.495C0
6805PPRO46.33266533-0.4255504860.8770.699C0
6806GGLY99.79959921.1395046130.6760.597C0
6807VVAL8.216432866-1.5412681760.7791.199E0
6808AALA27.89579158-0.9652246580.8210.865E0
6809MMET99.559118241.1324653850.6860.991C0
6810PPRO99.719438881.1371582040.9620.998C0
6811NASN8.817635271-1.5236701051.1871.165C0
6812LLEU66.172344690.1551858511.8441.861G0
6813YTYR82.404809620.6303337641.4491.38G0
6814KLYS99.759519041.1383314081.4451.18G0
6815MMET8.537074148-1.5318825391.8952.065G0
6816QGLN80.40080160.5716735282.2221.576C0
6817RARG43.56713427-0.5065016121.7391.989C0
6818MMET45.69138277-0.4443217612.5181.391C0
6819LLEU8.657314629-1.5283629242.711.79C0
6820LLEU69.779559120.2607742762.6891.546C0
6821EGLU93.867735470.9658703143.041.396C0
6822KLYS8.537074148-1.5318825393.1452.273C0
6823CCYS80.841683370.584578782.3851.074C0
6824DASP13.98797595-1.3723266962.5691.442C0
6825LLEU78.637274550.520052522.1941.992C0
6826QGLN8.056112224-1.5459609952.5971.473C0
6827NASN99.79959921.1395046133.8211.325C0
6828YTYR99.839679361.1406778185.4522.672C0
6829GGLY78.036072140.5024544494.4551.872C0
6830DASP2.645290581-1.7043436333.2272.559C1
6831SSER22.56513026-1.1212608862.682.034T2
6832AALA9.098196393-1.5154576723.1382.568T1
6833TTHR30.62124248-0.8854467373.9372.623T0
6834LLEU72.104208420.328820153.2571.869T0
6835PPRO95.310621241.0081056843.6721.56C0
6836KLYS8.216432866-1.5412681763.5122.585C0
6837GGLY99.478957921.1301189753.1841.837C0
6838IILE62.204408820.0390385842.4131.972C0
6839MMET71.102204410.2994900322.0391.784H0
6840MMET58.19639279-0.0782818881.8621.82H0
6841NASN99.879759521.1418510221.4661.325H0
6842VVAL82.685370740.6385461971.290.935H0
6843AALA58.31663327-0.0747622741.0150.735H0
6844KLYS99.879759521.1418510220.9030.89H0
6845YTYR99.839679361.1406778180.8370.755H0
6846TTHR77.87575150.497761630.7440.657H0
6847QGLN99.879759521.1418510220.5460.5H0
6848LLEU99.839679361.1406778180.5840.797H0
6849CCYS95.110220441.0022396610.6020.577H0
6850QGLN95.270541081.0069324790.5411.222H0
6851YTYR98.356713431.0972692430.5170.735H0
6852LLEU94.068136270.9717363380.4890.796H0
6853NASN77.394789580.4836831740.7361.026H0
6854TTHR47.25450902-0.3985667770.9920.958C0
6855LLEU8.256513026-1.5400949720.8251.046C0
6856TTHR94.028056110.9705631330.9080.966C0
6857LLEU50.34068136-0.3082300140.6721.072C0
6858AALA45.93186373-0.4372825330.7280.778C0
6859VVAL99.238476951.1230797470.7520.759C0
6860PPRO99.919839681.1430242270.8260.676C0
6861YTYR9.018036072-1.5178040820.8361.067C0
6862NASN69.859719440.2631206860.9471.463C0
6863MMET99.79959921.1395046130.7610.893E0
6864RARG95.35070141.0092788890.6991.049E0
6865VVAL99.559118241.1324653850.5830.744E0
6866IILE25.250501-1.042656170.7150.847E0
6867HHIS99.879759521.1418510220.6850.576E0
6868FPHE43.88777555-0.4971159740.6020.64E0
6869GGLY99.879759521.1418510220.7980.555E0
6870AALA99.879759521.1418510220.8320.749C0
6871GGLY90.30060120.8614550941.090.648C0
6872SSER94.629258520.9881612041.381.005T0
6873DASP78.797595190.5247453392.1311.44T0
6874KLYS87.855711420.7898896062.0951.943T0
6875GGLY97.835671341.0820175821.6060.826T0
6876VVAL78.917835670.5282649530.9011.305C0
6877AALA98.236472951.0937496290.8890.825H0
6878PPRO99.839679361.1406778181.0890.959H0
6879GGLY99.959919841.1441974321.1630.638H0
6880TTHR61.603206410.0214405130.8910.73H0
6881AALA52.50501002-0.2448769590.9810.84H0
6882VVAL99.959919841.1441974320.8980.775H0
6883LLEU95.470941881.0127985030.6620.733H0
6884RARG70.460921840.2807187570.8131.436H0
6885QGLN62.805611220.0566366550.9311.489H0
6886WTRP95.35070141.0092788890.8080.826H0
6887LLEU95.951903811.026876960.5890.784H0
6888PPRO99.839679361.1406778180.7980.775T0
6889TTHR24.76953908-1.0567346260.8981.339T0
6890GGLY44.52905812-0.4783446981.1050.964T0
6891TTHR40.84168337-0.5862795330.6930.822T0
6892LLEU25.69138277-1.0297509180.6531.103E0
6893LLEU51.50300601-0.2742070770.7030.785E0
6894VVAL73.707414830.3757483390.8110.826E0
6895DASP99.839679361.1406778180.7790.559E0
6896SSER8.256513026-1.5400949720.6840.694E0
6897DASP99.719438881.1371582040.920.728C0
6898LLEU41.88376754-0.555776210.9821.397C0
6899NASN36.87374749-0.70242682.3271.456C0
6900DASP60.961923850.0026692382.2131.367C0
6901FPHE44.8496994-0.4689590611.6310.752C0
6902VVAL94.468937880.9834683851.31.45C0
6903SSER99.879759521.1418510221.3550.782C0
6904DASP99.879759521.1418510221.7751.004C0
6905AALA85.05010020.7077652751.0420.827C0
6906DASP63.246492990.0695419070.8620.81C0
6907SSER8.336673347-1.5377485621.0990.833E0
6908TTHR39.55911824-0.6238220840.9490.86E0
6909LLEU26.17234469-1.0156724610.8771.516E0
6910IILE9.579158317-1.5013792161.1841.38C0
6911GGLY78.436873750.5141864961.090.876C0
6912DASP97.154308621.0620731011.0080.872T0
6913CCYS95.270541081.0069324791.1371.082T0
6914AALA15.03006012-1.3418233741.1830.994T0
6915TTHR74.188376750.3898267961.3711.136T0
6916VVAL32.74549098-0.8232668871.0741.208C0
6917HHIS8.657314629-1.5283629240.9971.354E0
6918TTHR25.65130261-1.0309241220.6850.84E0
6919AALA8.537074148-1.5318825390.7651.073C0
6920NASN8.456913828-1.5342289480.7180.976C0
6921KLYS63.086172340.0648490880.8741.118C0
6922WTRP24.00801603-1.0790255160.960.603C0
6923DASP99.959919841.1441974320.930.603E0
6924LLEU95.190380761.004586070.5581.206E0
6925IILE29.01803607-0.9323749260.5290.804E0
6926IILE91.703406810.9025172590.5490.965E0
6927SSER99.879759521.1418510220.6930.547E0
6928DASP99.839679361.1406778180.8690.606E0
6929MMET91.062124250.8837459840.7930.795C0
6930YTYR1001.1453706371.011.402C0
6931DASP78.396793590.5130132922.0191.667C0
6932PPRO40.4008016-0.5991847851.9262.18G0
6933KLYS5.0501002-1.6339513492.4343.365G0
6934TTHR41.60320641-0.5639886432.71.93G0
6935KLYS58.15631263-0.0794550932.7972.807G0
6936NASN34.34869739-0.7763386984.0242.628C0
6937VVAL26.05210421-1.0191920754.6062.853C0
6938TTHR23.80761523-1.084891544.3142.899C0
6939KLYS8.016032064-1.54713423.1283.309C0
6940EGLU41.00200401-0.5815867142.2812.554C0
6941NASN95.310621241.0081056841.9451.64C0
6942DASP9.458917836-1.504898831.5221.411C0
6943SSER76.633266530.4613922840.9970.741C0
6944KLYS78.07615230.5036276540.7010.926C0
6945EGLU35.63126253-0.7387961471.0991.42C0
6946GGLY60.921843690.0014960330.9290.913H0
6947FPHE83.006012020.6479318350.7230.894H0
6948FPHE99.879759521.1418510220.8180.716H0
6949TTHR51.34268537-0.2788998960.9010.956H0
6950YTYR94.589178360.9869879990.7951.055H0
6951IILE48.65731463-0.3575046120.7291.141H0
6952CCYS40.76152305-0.5886259420.6450.683H0
6953GGLY46.13226453-0.4314165090.6860.718H0
6954FPHE34.5490982-0.7704726740.7531.229H0
6955IILE78.396793590.5130132920.8330.943H0
6956QGLN2.565130261-1.7066900420.8141.183H0
6957QGLN8.296593186-1.5389217670.7311.251H0
6958KLYS60.48096192-0.0114092190.7210.83H0
6959LLEU99.238476951.1230797470.6760.72C0
6960AALA92.144288580.9154225110.5030.657T0
6961LLEU93.587174350.9576578810.5610.917T0
6962GGLY99.318637271.1254261560.5070.451T0
6963GGLY98.597194391.1043084710.4610.453T0
6964SSER72.42484970.3382057880.4060.471E0
6965VVAL69.939879760.2654670950.4510.729E0
6966AALA94.228456910.9764291570.4160.652E0
6967IILE78.116232460.5048008590.5360.907E0
6968KLYS99.39879761.1277725660.630.889E0
6969IILE57.75551102-0.091187140.7440.921E0
6970TTHR99.39879761.1277725660.90.807C0
6971EGLU99.358717431.1265993611.1081.242C0
6972HHIS31.46292585-0.8608094381.1491.166C0
6973SSER99.358717431.1265993610.8180.906C0
6974WTRP94.789579160.9928540231.2430.946C0
6975NASN59.5991984-0.0372197231.0110.704H0
6976AALA25.41082164-1.0379633511.0430.851H0
6977DASP14.34869739-1.3617678541.2290.917H0
6978LLEU98.717434871.1078280860.8880.938H0
6979YTYR99.318637271.1254261561.1030.905H0
6980KLYS19.63927856-1.2069048311.1871.359H0
6981LLEU80.440881760.5728467321.2521.127H0
6982MMET41.72344689-0.5604690290.8870.927T0
6983GGLY44.36873747-0.4830375170.9440.643T0
6984HHIS8.77755511-1.524843310.7430.853T0
6985FPHE82.404809620.6303337640.6690.564T0
6986AALA54.78957916-0.178004290.6790.738C0
6987WTRP42.64529058-0.533485320.7180.913E0
6988WTRP94.7494990.9916808180.670.872E0
6989TTHR94.7494990.9916808180.5480.771E0
6990AALA8.416833667-1.5354021530.7790.79E0
6991FPHE99.118236471.1195601330.8320.661E0
6992VVAL8.336673347-1.5377485620.6580.794E0
6993TTHR98.637274551.1054816760.7920.649T0
6994NASN40.44088176-0.598011580.8880.584T0
6995VVAL70.260521040.2748527331.1340.804T0
6996NASN99.278557111.1242529520.9781.073T0
6997AALA62.084168340.035518971.6121.224C0
6998SSER98.917835671.1136941091.5811.279C0
6999SSER99.278557111.1242529521.3620.808C0
7000SSER99.238476951.1230797471.5740.962C0
7001EGLU99.318637271.1254261561.0860.998C0
7002AALA61.563126250.0202673081.0450.809E0
7003FPHE98.917835671.1136941090.840.731E0
7004LLEU95.751503011.0210109360.6911.325E0
7005IILE62.244488980.0402117890.5930.865E0
7006GGLY94.7494990.9916808180.5030.517E0
7007CCYS2.605210421-1.7055168370.5950.669E0
7008NASN75.871743490.4391013940.6090.612C0
7009YTYR96.472945891.0421286210.6560.566C0
7010LLEU98.757515031.109001290.7490.792C0
7011GGLY91.142284570.8860923930.9820.732C0
7012KLYS22.24448898-1.1306465240.8781.84C0
7013PPRO18.23647295-1.2479669961.0251.121C0
7014RARG5.090180361-1.6327781451.5712.216C0
7015EGLU29.81963928-0.9089108311.5331.66C0
7016QGLN8.176352705-1.5424413812.1042.122C0
7017IILE76.272545090.4508334411.4981.083C0
7018DASP75.310621240.4226765281.3191.277H0
7019GGLY99.118236471.1195601331.3090.891H0
7020YTYR8.456913828-1.5342289481.5341.623H0
7021VVAL18.91783567-1.2280225161.8161.148H0
7022MMET71.262525050.3041828511.6790.903H0
7023HHIS99.118236471.1195601331.7730.895H0
7024AALA99.038076151.1172137231.6070.844H0
7025NASN94.669338680.9893344090.9970.762H0
7026YTYR99.078156311.1183869280.8421.023H0
7027IILE80.841683370.584578781.5941.01H0
7028FPHE94.589178360.9869879991.880.934H0
7029WTRP99.078156311.1183869281.2460.824H0
7030RARG99.158316631.1207333371.1641.038H0
7031NASN99.038076151.1172137231.8370.983H0
7032TTHR8.617234469-1.5295361291.9881.006H0
7033NASN24.60921844-1.0614274451.8651.276C0
7034PPRO25.21042084-1.0438293743.1251.883C0
7035IILE8.256513026-1.5400949720.8831.521C0
7036QGLN25.41082164-1.0379633510.8631.83C0
7037LLEU36.99398798-0.6989071860.9621.5C0
7038SSER79.879759520.5564218661.0450.742C0
7039SSER9.579158317-1.5013792161.4420.892G0
7040YTYR57.75551102-0.091187142.1231.177G0
7041SSER94.428857720.982295182.0680.986G0
7042LLEU46.01202405-0.4349361242.0651.33G0
7043FPHE57.79559118-0.0900139351.8221.588G0
7044DASP99.038076151.1172137231.7411.582T0
7045MMET23.08617234-1.1060092251.5081.925T0
7046SSER56.99398798-0.113478032.0691.967T0
7047KLYS92.745490980.9330205822.1642.714T0
7048FPHE94.428857720.982295181.3580.98C0
7049PPRO22.04408818-1.1365125471.111.025C0
7050LLEU58.27655311-0.0759354790.9110.875C0
7051KLYS89.4989980.8379911.1781.904C0
7052LLEU59.43887776-0.0419125420.8361.106C0
7053RARG8.69739479-1.527189720.9431.224C0
7054GGLY41.16232465-0.5768938950.6930.677C0
7055TTHR94.54909820.9858147940.8040.712C0
7056AALA23.76753507-1.0860647441.2570.834E0
7057VVAL90.741482970.8743603461.1951.243E0
7058MMET9.138276553-1.5142844680.6911.225E0
7059SSER16.39278557-1.3019344130.9421.18C0
7060LLEU94.589178360.9869879991.2151.376C0
7061KLYS82.605210420.6361997871.4892.194T0
7062EGLU36.47294589-0.7141588472.0722.335T0
7063GGLY4.048096192-1.6632814672.4971.851T0
7064QGLN57.03406814-0.1123048252.2031.773T0
7065IILE65.290581160.1293753481.441.301C0
7066NASN52.78557114-0.2366645261.5851.182H1
7067DASP70.140280560.2713331191.8311.378H1
7068MMET30.78156313-0.8807539181.3811.842H0
7069IILE8.897795591-1.5213236960.9971.126H0
7070LLEU36.43286573-0.7153320521.4021.339H0
7071SSER45.65130261-0.4454949661.4680.973H0
7072LLEU98.677354711.1066548811.1150.922H0
7073LLEU74.388777560.3956928191.2380.807H0
7074SSER20.12024048-1.1928263741.9770.768H0
7075KLYS25.81162325-1.0262313032.1241.641H0
7076GGLY98.557114231.1031352671.5520.753C0
7077RARG17.03406814-1.2831631381.5251.445E0
7078LLEU95.55110221.0151449130.7471.059E0
7079IILE9.338677355-1.5084184440.480.983E0
7080IILE49.97995992-0.3187888560.6540.795E0
7081RARG98.396793591.0984424480.8340.868E0
7082EGLU8.296593186-1.5389217671.9691.435C0
7083NASN58.83767535-0.0595106133.5442.007C0
7084NASN19.75951904-1.2033852162.8381.881C0
7085RARG9.97995992-1.4896471692.1452.356C0
7086VVAL21.9238477-1.1400321611.0631.21C0
7087VVAL21.76352705-1.144724980.7941.436E0
7088IILE2.845691383-1.6984776090.8051.037E0
7089SSER26.21242485-1.0144992560.8771.15E0
7090SSER51.50300601-0.2742070771.1611.379C0
7091DASP56.51302605-0.1275564872.2512.555C0
7092VVAL21.08216433-1.1646694612.8083.131C0
7093LLEU70.661322650.286584786.265.969C1
7094VVAL89.258517030.8309517718.5436.554C2
7095NASN68.617234470.22675133913.1848.015C2
7096NASN7.895791583-1.55065381417.17210.143C1

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27.         View of A cohort of cancer patients with no reported cases of SARS-CoV-2 infection: the possible preventive role of Methylene Blue | Substantia [Internet]. [cited 2020 Apr 8];Available from: https://riviste.fupress.net/index.php/subs/article/view/888/548