Genes and Variants to Function

The lab focuses on developing a knowledge of either genes or variants to functional outcomes in cells and organisms for the broad understanding of life and the details of how multiple diseases develop. The lab starts with computational analysis to build working hypotheses that are then tested using biochemical, cellular, and organism methods.

Workflow - Copy

Computational Strategies

The lab has developed a workflow for a sequence-to-structure-to-function-to-phenotype understanding of genes that uses a combination of evolutionary biology, structural chemistry, and expression profiling.

Biochemistry

Using multiple biochemical techniques we then test hypotheses for protein-protein and protein-DNA interaction

Figure 3AB
Podocyte stained red for actin and blue for the nucleus

Cell Culture

The detailed knowledge of protein function is then translated into cellular mechanisms using a variety of cell culture techniques